Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	701,574	A→G	A7A (GCT→GCC)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	701,574	0	A	G	100.0%	120.5 / NA	34	A7A (GCT→GCC)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/23);  total (11/23)

CGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGC  >  NC_000913/701434‑701721                                                                                                                                             |                                                                                                                                                    cgcgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGc                                                                                                                                           >  1:544151/1‑151 (MQ=255)   cgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCt                                                                                                                                         <  1:582485/151‑1 (MQ=255)             gTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATAc                                                                                                                               <  1:365760/151‑1 (MQ=255)              ttACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACt                                                                                                                              >  2:575000/1‑151 (MQ=255)               tACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACt                                                                                                                              <  1:342626/150‑1 (MQ=255)                aCGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACtt                                                                                                                             <  1:387032/150‑1 (MQ=255)                       cGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTAt                                                                                                                     <  1:575015/151‑1 (MQ=255)                         ggggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTAtt                                                                                                                    <  2:180901/150‑1 (MQ=255)                          gggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGa                                                                                                                  <  2:580288/151‑1 (MQ=255)                                   ggCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGAc                                                                                                          <  1:297640/150‑1 (MQ=255)                                               cAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtctc                                                                                                                         >  1:192967/1‑123 (MQ=255)                                               cAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtctc                                                                                                                         <  2:192962/123‑1 (MQ=255)                                                     gAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACg                                                                                          <  2:112554/148‑1 (MQ=255)                                                             gagaTTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGt                                                                               <  2:165988/151‑1 (MQ=255)                                                                  tGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGAttt                                                                          >  1:181126/1‑151 (MQ=255)                                                                     cccGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTaa                                                                       <  2:330118/151‑1 (MQ=255)                                                                        gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAAt                                                                    <  1:344371/151‑1 (MQ=255)                                                                                        cGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGt                                                    <  1:258178/151‑1 (MQ=255)                                                                                            aaaCCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTGCCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCa                                                  <  2:532304/149‑1 (MQ=255)                                                                                               ccgccgCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTg                                             <  1:457821/151‑1 (MQ=255)                                                                                                 gccgcGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGc                                           <  2:486147/151‑1 (MQ=255)                                                                                                  ccgcGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGATTGCAGTCAGGTTGGCt                                          <  2:279006/151‑1 (MQ=255)                                                                                                  ccgcGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCt                                          <  2:328262/151‑1 (MQ=255)                                                                                                     cgcTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACt                                       >  1:130418/1‑151 (MQ=255)                                                                                                        tGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACttt                                     >  1:273763/1‑150 (MQ=255)                                                                                                              gCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgcg                              <  2:519050/151‑1 (MQ=255)                                                                                                              gCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgcg                              >  2:485714/1‑151 (MQ=255)                                                                                                                      aCGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAgtgt                      >  2:412867/1‑151 (MQ=255)                                                                                                                      aCGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAgtg                       <  1:260542/150‑1 (MQ=255)                                                                                                                        gagaTCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGc                    >  1:155840/1‑151 (MQ=255)                                                                                                                          gaTCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGGGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGcc                   >  2:430371/1‑150 (MQ=255)                                                                                                                              aaCATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGAc              <  2:294609/151‑1 (MQ=255)                                                                                                                                 aTTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGtt           <  2:551219/151‑1 (MQ=255)                                                                                                                                          cTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGc  >  2:513625/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                    CGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGC  >  NC_000913/701434‑701721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑