Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	706,673	T→C	I194T (ATT→ACT)	glnS →	glutamyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	706,673	0	T	C	100.0%	139.7 / NA	38	I194T (ATT→ACT)	glnS	glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

CGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACT  >  NC_000913/706526‑706819                                                                                                                                                    |                                                                                                                                                   cGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTa                                                                                                                                                  >  2:406144/1‑150 (MQ=255)   tACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAAt                                                                                                                                               >  2:206598/1‑151 (MQ=255)      cgcgACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTg                                                                                                                                            <  2:203393/151‑1 (MQ=255)               aGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAAcacc                                                                                                                                   >  2:223570/1‑151 (MQ=255)                gCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAAcacca                                                                                                                                  <  2:9792/151‑1 (MQ=255)                     gAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAg                                                                                                                              <  2:316269/150‑1 (MQ=255)                      aaGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGAc                                                                                                                            >  2:242256/1‑151 (MQ=255)                        gagaACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTg                                                                                                                          >  2:546827/1‑151 (MQ=255)                                            aaaaaTGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTAc                                                                                                      <  1:412112/151‑1 (MQ=255)                                              aaaTGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTAccc                                                                                                    >  1:217844/1‑151 (MQ=255)                                               aaTGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCg                                                                                                   >  1:288653/1‑151 (MQ=255)                                                      gCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACGAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACg                                                                                            <  2:308786/151‑1 (MQ=255)                                                       ccGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACg                                                                                            >  2:387714/1‑150 (MQ=255)                                                               tttGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCAccc                                                                                   >  2:98635/1‑151 (MQ=255)                                                               tttGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCAccc                                                                                   >  2:589751/1‑151 (MQ=255)                                                                ttGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCAccc                                                                                   <  2:238503/150‑1 (MQ=255)                                                                        ggTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCa                                                                          <  2:76049/151‑1 (MQ=255)                                                                        ggTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCa                                                                          <  2:448001/151‑1 (MQ=255)                                                                           aaaGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCg                                                                       >  1:216559/1‑151 (MQ=255)                                                                            aaGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGa                                                                      <  1:366413/151‑1 (MQ=255)                                                                             aGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGAt                                                                     >  1:204855/1‑151 (MQ=255)                                                                                       cgtgcgAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGa                                                             >  1:385107/1‑149 (MQ=255)                                                                                            gAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTAtt                                                      >  1:540466/1‑151 (MQ=255)                                                                                            gAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACCTCACCCACTGCATCAGCGATGCCCTGGAAGGTAtt                                                      <  2:382658/151‑1 (MQ=255)                                                                                                  cGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCa                                                 <  2:153685/150‑1 (MQ=255)                                                                                                            tCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTgtgt                                       <  1:406151/150‑1 (MQ=255)                                                                                                              aCCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACg                                    <  1:85306/151‑1 (MQ=255)                                                                                                                cGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCt                                  <  1:402223/151‑1 (MQ=255)                                                                                                                    tATCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGa                               <  1:604776/150‑1 (MQ=255)                                                                                                                     aTCGTGATGCGCGATCCGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAg                              <  2:256571/150‑1 (MQ=255)                                                                                                                                    ccGGTGCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACcgt               <  1:206603/150‑1 (MQ=255)                                                                                                                                     cGGTGCTGTACCGTACTAAATATGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACcgtcg             >  2:97571/1‑151 (MQ=255)                                                                                                                                         gCTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTg         <  1:509563/151‑1 (MQ=255)                                                                                                                                          cTGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGt        >  2:80712/1‑151 (MQ=255)                                                                                                                                           tGTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGt        >  2:405688/1‑150 (MQ=255)                                                                                                                                            gTACCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTa       <  2:433532/150‑1 (MQ=255)                                                                                                                                              aCCGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACg     >  2:592747/1‑150 (MQ=255)                                                                                                                                                cGTACTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACt  >  2:612668/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGAACACCACCAGACTGGCAACAAGTGGTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACT  >  NC_000913/706526‑706819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑