Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	741,429	A→G	S376S (TCT→TCC)	dtpD ←	dipeptide and tripeptide permease D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	741,429	0	A	G	100.0%	124.4 / NA	34	S376S (TCT→TCC)	dtpD	dipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/21);  total (13/21)

GATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCC  >  NC_000913/741282‑741573                                                                                                                                                    |                                                                                                                                                 gATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGggaa                                                                                                                                               <  2:23806/151‑1 (MQ=255)         cAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAcc                                                                                                                                       >  2:72489/1‑151 (MQ=255)          aGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAcc                                                                                                                                       >  2:502226/1‑150 (MQ=255)           gAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAcc                                                                                                                                       >  2:492245/1‑149 (MQ=255)                cagcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACAtt                                                                                                                                <  1:380147/151‑1 (MQ=255)                 agcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTg                                                                                                                               <  1:279491/151‑1 (MQ=255)                 agcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTg                                                                                                                               >  2:574704/1‑151 (MQ=255)                      gTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGa                                                                                                                           <  1:488494/150‑1 (MQ=255)                       tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGATGAGTGACCATACATTGCGGAcc                                                                                                                         >  2:82599/1‑151 (MQ=255)                                  tAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCt                                                                                                               <  2:147850/150‑1 (MQ=255)                                    aTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCTGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCtt                                                                                                              >  1:14634/1‑149 (MQ=255)                                      aCGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAgg                                                                                                          >  1:631327/1‑151 (MQ=255)                                         ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTc                                                                                                        >  1:326865/1‑150 (MQ=255)                                                        aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCgg                                                                                        <  2:111612/151‑1 (MQ=255)                                                                    gTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggc                                                                            <  2:195916/151‑1 (MQ=255)                                                                        tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggcca                                                                          >  1:41242/1‑149 (MQ=255)                                                                              aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAggccaaggccaa                                                                   <  2:201287/150‑1 (MQ=255)                                                                                             cGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCa                                                   <  1:621976/151‑1 (MQ=255)                                                                                                  aaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgc                                              <  2:135958/151‑1 (MQ=255)                                                                                                   aCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcg                                             <  1:319474/151‑1 (MQ=255)                                                                                                    cAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcgc                                            >  2:576309/1‑151 (MQ=255)                                                                                                      gTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCAc                                          <  2:311671/151‑1 (MQ=255)                                                                                                         ccGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGt                                       >  1:130905/1‑151 (MQ=255)                                                                                                           gCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTAc                                     <  1:621547/151‑1 (MQ=255)                                                                                                                    aTCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTAccc                             >  1:122290/1‑150 (MQ=255)                                                                                                                      cACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCcgcg                          <  2:88336/151‑1 (MQ=255)                                                                                                                      cACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCcgc                           <  2:468095/150‑1 (MQ=255)                                                                                                                             aGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActct                   <  1:537016/151‑1 (MQ=255)                                                                                                                              ggCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActctc                  <  1:42211/151‑1 (MQ=255)                                                                                                                                ccTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCtt                <  1:379201/151‑1 (MQ=255)                                                                                                                                   aaTACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCtttt              <  1:482791/150‑1 (MQ=255)                                                                                                                                       ccATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTacca          <  2:160561/150‑1 (MQ=255)                                                                                                                                        cATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTaccacc        <  2:297846/151‑1 (MQ=255)                                                                                                                                            aGTGGCAGGGAAGAGTGACCATACATTTCGGGCCAGCGGGCGCTTAAGGGCAGAATGCAGAATCCGGCGCTCATCAGGCCAAAGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGGTTACCCCCCACACTCTCTTTTAccacccac     >  2:284058/1‑150 (MQ=255)                                                                                                                                               ggCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGcc  <  1:565796/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCC  >  NC_000913/741282‑741573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑