Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	747,436	T→C	T112A (ACC→GCC)	abrB ←	regulator of aidB expression; inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	747,436	0	T	C	100.0%	198.8 / NA	53	T112A (ACC→GCC)	abrB	regulator of aidB expression; inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

TCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGC  >  NC_000913/747289‑747582                                                                                                                                                    |                                                                                                                                                   tcatGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCAtt                                                                                                                                                 >  2:565854/1‑151 (MQ=255)     gCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTcc                                                                                                                                              <  2:377254/150‑1 (MQ=255)           gACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAAt                                                                                                                                       >  1:237445/1‑151 (MQ=255)                     gCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAgc                                                                                                                             >  2:368384/1‑151 (MQ=255)                         cccccGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCAc                                                                                                                         <  1:273963/151‑1 (MQ=255)                                gacgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATaac                                                                                                                  <  1:278231/151‑1 (MQ=255)                                gacgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATaac                                                                                                                  >  2:346107/1‑151 (MQ=255)                                 acgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATaacc                                                                                                                 >  2:34828/1‑151 (MQ=255)                                         cACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCcac                                                                                                          >  2:558102/1‑150 (MQ=255)                                            tCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTc                                                                                                                                               <  2:554470/110‑1 (MQ=255)                                            tCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTc                                                                                                                                               >  1:554481/1‑110 (MQ=255)                                            tCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATg                                                                                                      >  2:594617/1‑151 (MQ=255)                                              gCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCctca                                                                                                         <  2:246240/146‑1 (MQ=255)                                               cAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGgcg                                                                                                   <  2:435234/151‑1 (MQ=255)                                                   tatTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTgg                                                                                               >  2:446547/1‑151 (MQ=255)                                                       gCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGaaa                                                                                            <  1:368391/150‑1 (MQ=255)                                                        cATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAagc                                                                                          >  1:604787/1‑151 (MQ=255)                                                              cGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGtt                                                                                    <  2:382535/151‑1 (MQ=255)                                                               gCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTa                                                                                   <  1:221734/151‑1 (MQ=255)                                                                  accaGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGCAAAGCAGCGTTACCa                                                                                <  1:79868/151‑1 (MQ=255)                                                                   ccaGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTAcc                                                                                 >  2:456613/1‑149 (MQ=255)                                                                    caGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGt                                                                              >  1:620824/1‑151 (MQ=255)                                                                    caGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGt                                                                              <  1:258467/151‑1 (MQ=255)                                                                     aGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAg                                                                               >  1:488197/1‑149 (MQ=255)                                                                     aGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGt                                                                              >  2:236388/1‑150 (MQ=255)                                                                          aaTATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAAtt                                                                        <  1:431151/151‑1 (MQ=255)                                                                             atCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGc                                                                      >  2:56725/1‑150 (MQ=255)                                                                              tCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTg                                                                       >  2:196742/1‑148 (MQ=255)                                                                               cTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCa                                                                     >  2:137171/1‑149 (MQ=255)                                                                                   gcCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACg                                                               <  2:604772/151‑1 (MQ=255)                                                                                      gTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATc                                                            >  1:346682/1‑151 (MQ=255)                                                                                       tAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAagca                                                                                         <  2:264952/121‑1 (MQ=255)                                                                                       tAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAagca                                                                                         >  1:264959/1‑121 (MQ=255)                                                                                        aaTCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCg                                                           <  2:615944/150‑1 (MQ=255)                                                                                               cgcCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTg                                                   <  2:235947/151‑1 (MQ=255)                                                                                                 ccATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGAc                                                 >  2:570309/1‑151 (MQ=255)                                                                                                  cATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACg                                                <  2:244928/151‑1 (MQ=255)                                                                                                  cATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACg                                                <  2:454893/151‑1 (MQ=255)                                                                                                    tAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGc                                              >  1:384487/1‑151 (MQ=255)                                                                                                     aGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAACACGATCGGCCAGTTGACGGcc                                             >  2:92214/1‑151 (MQ=255)                                                                                                           cATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGggtg                                       <  2:300796/151‑1 (MQ=255)                                                                                                           cATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGggtg                                       <  2:592/151‑1 (MQ=255)                                                                                                            aTTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGggtgg                                      >  1:282408/1‑151 (MQ=255)                                                                                                               gccgccGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGgtggtg                                    >  1:251715/1‑150 (MQ=255)                                                                                                                 cgccgcgccgCCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGgtggtg                                    >  2:231758/1‑148 (MQ=255)                                                                                                                      cgccgcCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCg                             >  2:622896/1‑150 (MQ=255)                                                                                                                      cgccgcCAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGa                            <  2:590542/151‑1 (MQ=255)                                                                                                                           ccAGGAGAGGAGCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAg                       <  2:188597/151‑1 (MQ=255)                                                                                                                             aggagaggagCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTg                     >  2:130213/1‑151 (MQ=255)                                                                                                                             aggagaggagCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTg                     <  2:619671/151‑1 (MQ=255)                                                                                                                                agaggagCCCCAGGCACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAgg                  <  2:368703/151‑1 (MQ=255)                                                                                                                                              cACCGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCAt    >  2:600358/1‑151 (MQ=255)                                                                                                                                                ccGGCATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGc  >  1:462343/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGC  >  NC_000913/747289‑747582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑