Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	771,344	T→C	intergenic (+733/‑114)	mngB → / → cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	771,344	0	T	C	100.0%	93.9 / NA	27	intergenic (+733/‑114)	mngB/cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/10);  total (17/10)

GATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACA  >  NC_000913/771209‑771489                                                                                                                                        |                                                                                                                                                  gATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTat                                                                                                                                    <  1:484227/151‑1 (MQ=255) gATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTa                                                                                                                                     <  2:143687/150‑1 (MQ=255)        gTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGaaa                                                                                                                             <  2:433229/151‑1 (MQ=255)         tCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGaa                                                                                                                              >  2:55931/1‑149 (MQ=255)          cGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAgt                                                                                                                           <  2:556354/151‑1 (MQ=255)           gAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAgtg                                                                                                                          <  2:123451/151‑1 (MQ=255)             aaaaTGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGtt                                                                                                                        >  2:12873/1‑151 (MQ=255)                    aaaTTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTcaaaca                                                                                                                  >  1:134178/1‑150 (MQ=255)                        ttGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAAttt                                                                                                             >  1:443326/1‑151 (MQ=255)                             tCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAtt                                                                                                        >  1:75139/1‑151 (MQ=255)                                     aaCCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATg                                                                                                >  2:552047/1‑151 (MQ=255)                                            ttATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGtgtg                                                                                          >  1:584328/1‑150 (MQ=255)                                             tATTGCAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGAc                                                                                        >  2:41448/1‑151 (MQ=255)                                                   aaGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTc                                                                                  <  1:179064/151‑1 (MQ=255)                                                   aaGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTc                                                                                  >  1:357534/1‑151 (MQ=255)                                                          ttGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACggg                                                                           >  1:461138/1‑151 (MQ=255)                                                                          aCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGt                                                            <  2:514169/150‑1 (MQ=255)                                                                                gATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATg                                                     <  2:452616/151‑1 (MQ=255)                                                                                    aaTTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGAt                                                 >  1:497334/1‑151 (MQ=255)                                                                                           gTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCa                                            >  1:569343/1‑149 (MQ=255)                                                                                             ttttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAgg                                         >  2:296158/1‑150 (MQ=255)                                                                                                     aCATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCatgat                                >  1:44988/1‑151 (MQ=255)                                                                                                                    ttctttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTc                   <  2:492895/149‑1 (MQ=255)                                                                                                                       tttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACt              >  2:494693/1‑151 (MQ=255)                                                                                                                         tACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGt            >  2:574893/1‑151 (MQ=255)                                                                                                                          aCGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTc           <  2:443318/151‑1 (MQ=255)                                                                                                                             ccGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTcgcg        >  2:374301/1‑151 (MQ=255)                                                                                                                                    tgtgcgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACa  <  2:525506/150‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                  GATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACA  >  NC_000913/771209‑771489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑