Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	95,768	A→G	V373V (GTA→GTG)	murF →	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	95,768	0	A	G	100.0%	130.4 / NA	35	V373V (GTA→GTG)	murF	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/16);  total (19/16)

ACCAGTTGCTGCTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTACAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATT  >  NC_000913/95623‑95912                                                                                                                                                  |                                                                                                                                                 aCCAGTTGCTGCTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGGGCTGAAAGCGAAGCCTGCCATGTGCAgg                                                                                                                                              >  2:250031/1‑150 (MQ=255)              cgacgaCTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCCTGTGCAGGCGGGCGAggcggc                                                                                                                                 >  2:486727/1‑150 (MQ=255)                  gacTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCaaa                                                                                                                              >  1:198868/1‑149 (MQ=255)                   acTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAgc                                                                                                                           <  1:627394/151‑1 (MQ=255)                     tCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAgctg                                                                                                                         >  1:66715/1‑151 (MQ=255)                          cAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGt                                                                                                                    <  1:171282/151‑1 (MQ=255)                           aaCGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGCGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCCGGTGGGCGAGGCGGCAAAAGCTGCTGGt                                                                                                                    >  1:362756/1‑150 (MQ=255)                            aCGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTAt                                                                                                                  >  1:57453/1‑151 (MQ=255)                             cGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTAtt                                                                                                                 >  2:275236/1‑151 (MQ=255)                              gCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTg                                                                                                                >  1:576030/1‑151 (MQ=255)                                    gTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACcgc                                                                                                           <  2:396803/150‑1 (MQ=255)                                             aTGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCg                                                                                                 <  1:178092/151‑1 (MQ=255)                                              tGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCg                                                                                                 >  1:15829/1‑150 (MQ=255)                                                   gcagcaGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGt                                                                                            <  2:291063/150‑1 (MQ=255)                                                        agTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACa                                                                                       >  1:363968/1‑150 (MQ=255)                                                            cAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGcc                                                                                  >  1:445763/1‑151 (MQ=255)                                                               gTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATg                                                                               >  2:79127/1‑151 (MQ=255)                                                                    ggCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTAt                                                                           <  2:198863/150‑1 (MQ=255)                                                                     gCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTAt                                                                           >  2:484547/1‑149 (MQ=255)                                                                          aaTGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCAcc                                                                    >  2:182641/1‑151 (MQ=255)                                                                          aaTGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCAcc                                                                    <  2:57453/151‑1 (MQ=255)                                                                                    tACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGGGGGTAAACAAAGCCATGCTATCAGCACCGCCAgcggcg                                                          >  2:437617/1‑151 (MQ=255)                                                                                      ccGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGt                                                         <  1:102780/150‑1 (MQ=255)                                                                                         cgTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGc                                                     <  1:321184/151‑1 (MQ=255)                                                                                          gTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCg                                                    <  2:223537/151‑1 (MQ=255)                                                                                             cTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGaa                                                  >  1:22633/1‑150 (MQ=255)                                                                                               ggtggtggGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAAc                                                 <  2:561704/149‑1 (MQ=255)                                                                                               ggtggtggGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACAt                                               <  2:353285/151‑1 (MQ=255)                                                                                                           tatGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATaa                                    >  1:270757/1‑150 (MQ=255)                                                                                                                 ggAACTGGGCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCg                             >  1:188768/1‑151 (MQ=255)                                                                                                                       gggCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGGGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCTCCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAAt                        <  2:247022/150‑1 (MQ=255)                                                                                                                        ggCGCTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTa                      <  2:187553/151‑1 (MQ=255)                                                                                                                         gcgcTGAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTAc                     <  1:437625/151‑1 (MQ=255)                                                                                                                              gAAAGCGAAGCCTGCCATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTc                <  2:203403/151‑1 (MQ=255)                                                                                                                                            ccATGTGCAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGAtt  >  1:464511/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                 ACCAGTTGCTGCTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATGTACAGGTGGGCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATT  >  NC_000913/95623‑95912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑