Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	781,084	T→C	noncoding (17/76 nt)	valZ →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,084	0	T	C	100.0%	98.8 / NA	32	noncoding (17/76 nt)	valZ	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

GATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTT  >  NC_000913/780937‑781226                                                                                                                                                    |                                                                                                                                               gATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCggg                                                                                                                                             >  1:371990/1‑151 (MQ=255)  aTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGga                                                                                                                                            <  2:195761/151‑1 (MQ=255)                    aaCGTTGCATCAGCAACGGCCCGTGGGGTATGCGACGCGAGTCATCCTGCACGACCCCCCTCTAAGTGCGGTGGGGACGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGGACCGCCCt                                                                                                                                <  2:618764/145‑1 (MQ=21)                       gTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGACAGCACCTCCCTTACa                                                                                                                            >  1:194817/1‑146 (MQ=255)                          gCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAgggg                                                                                                                    <  2:170216/151‑1 (MQ=255)                                   aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACTACCCTCCACTAATGACGCTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGt                                                                                                           <  2:508994/151‑1 (MQ=255)                                   aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGt                                                                                                           >  1:339675/1‑151 (MQ=255)                                         catAGGGCGAGGCACGCGGGTCGTGATGAACGCCCCTCCACTAATGCCGGTGGCTTCGATGGTAGTAGGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                                                                                                     <  2:121457/149‑1 (MQ=17)                                                     gaagcgaGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccc                                                                                         >  1:137857/1‑151 (MQ=255)                                                               aTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCggg                                                                               <  2:533236/151‑1 (MQ=255)                                                                           ccaccaCTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                                     >  2:595709/1‑149 (MQ=255)                                                                              ccacTAATGACGGTGGGTTCGGTGGAAGTTGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTg                                                                <  1:620859/151‑1 (MQ=255)                                                                                      gACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCa                                                        <  1:321093/151‑1 (MQ=255)                                                                                        cGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                      <  1:547332/151‑1 (MQ=255)                                                                                        cGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGGAGAGCAg                                                       >  1:7664/1‑150 (MQ=255)                                                                                                     ggAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGGAGTTGACTTTTAAt                                           >  2:23088/1‑149 (MQ=255)                                                                                                         gtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTg                                     >  1:183915/1‑151 (MQ=255)                                                                                                            gtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                   <  1:87708/150‑1 (MQ=38)                                                                                                             tttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg                                 >  1:148375/1‑151 (MQ=37)                                                                                                              ttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                    <  2:560937/147‑1 (MQ=25)                                                                                                              ttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGGTGATTTTTAATCAATTGGTCGc                                >  1:53164/1‑151 (MQ=255)                                                                                                                gtagtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                              <  1:145317/151‑1 (MQ=25)                                                                                                                  agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGt                            >  2:341398/1‑151 (MQ=25)                                                                                                                  agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGt                            <  2:148371/151‑1 (MQ=25)                                                                                                                      tCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                         >  2:177624/1‑150 (MQ=25)                                                                                                                             cAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                 <  1:514702/151‑1 (MQ=21)                                                                                                                                   cGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAcc           >  2:516468/1‑151 (MQ=18)                                                                                                                                    gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCCGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc          >  1:574699/1‑151 (MQ=18)                                                                                                                                    gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc          >  2:507768/1‑151 (MQ=18)                                                                                                                                          ttAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGt    >  2:330743/1‑151 (MQ=18)                                                                                                                                            aGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGtt   <  2:183910/150‑1 (MQ=18)                                                                                                                                               tCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCCCGGTCGTTAGCTCAGGTGGTAGAGCAGATGACTTTTAATCAATTGGTTGCAGGGTCGAATCCTGCACGACCCACCAtttt  >  2:602286/1‑148 (MQ=11)                                                                                                                                                    |                                                                                                                                               GATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTT  >  NC_000913/780937‑781226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑