Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,415	C→T	A299V (GCC→GTC)	gsiA →	glutathione ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,415	0	C	T	100.0%	101.4 / NA	27	A299V (GCC→GTC)	gsiA	glutathione ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)

AAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTCAGTTTTGATC  >  NC_000913/868265‑868558                                                                                                                                                       |                                                                                                                                                aaaCGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGc                                                                                                                                                 <  1:261153/151‑1 (MQ=255)                 aCAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAg                                                                                                                                 <  1:462783/151‑1 (MQ=255)                     aTTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGaaaa                                                                                                                             <  2:393794/151‑1 (MQ=255)                           cATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACggtgg                                                                                                                       <  1:73188/151‑1 (MQ=255)                                 ccGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATg                                                                                                                 <  1:151555/151‑1 (MQ=255)                                  cGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATgg                                                                                                                <  1:372283/151‑1 (MQ=255)                                    cAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCg                                                                                                              <  2:137003/151‑1 (MQ=255)                                        aTCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                                          >  2:557866/1‑151 (MQ=255)                                                    gtggggtGTTAGCGGGGGTTCGGCAACTTGGGGGGGTGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAc                                                                                                           <  2:503760/132‑1 (MQ=255)                                                      gcgcTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGc                                                                                            >  2:395151/1‑151 (MQ=255)                                                              aGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCt                                                                                     <  1:30043/150‑1 (MQ=255)                                                                   ctgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAc                                                                               >  1:269483/1‑151 (MQ=255)                                                                             aCTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCt                                                                     <  2:442267/151‑1 (MQ=255)                                                                                          aaaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGtt                                                        <  2:364128/151‑1 (MQ=255)                                                                                               gTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgttg                                                   <  2:20175/151‑1 (MQ=255)                                                                                                      tATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATcgcg                                            >  1:177894/1‑151 (MQ=255)                                                                                                       aTCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATcgcg                                            <  2:66964/150‑1 (MQ=255)                                                                                                       aTCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGt                                           >  1:623344/1‑151 (MQ=255)                                                                                                          cccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAAc                                        <  1:501456/151‑1 (MQ=255)                                                                                                                   tCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAg                                <  2:133849/150‑1 (MQ=255)                                                                                                                         tGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGc                         >  2:465418/1‑151 (MQ=255)                                                                                                                         tGATATCGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGc                         >  1:197843/1‑151 (MQ=255)                                                                                                                               cGCTTGAACATCCAGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTg                    >  1:474097/1‑150 (MQ=255)                                                                                                                                            aGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATcg                                              >  2:209547/1‑111 (MQ=255)                                                                                                                                            aGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATcg                                              <  1:209552/111‑1 (MQ=255)                                                                                                                                            aGCGAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTCAGttt       >  1:459250/1‑150 (MQ=255)                                                                                                                                               gAAACAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTCAGTTTTGAt   >  1:529291/1‑151 (MQ=255)                                                                                                                                                aaaCAGGTCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTCAGTTTTGAtc  >  1:56420/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                AAACGGGTACCGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTCAGTTTTGATC  >  NC_000913/868265‑868558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑