Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	929,067	G→A	L43L (CTC→CTT)	cydC ←	glutathione/cysteine ABC transporter export permease/ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	929,067	0	G	A	100.0%	111.3 / NA	29	L43L (CTC→CTT)	cydC	glutathione/cysteine ABC transporter export permease/ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/12);  total (17/12)

AACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAGAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCAAAGCGCGCATTTAAATC  >  NC_000913/928921‑929202                                                                                                                                                   |                                                                                                                                        aaCACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAgg                                                                                                                                      >  1:437211/1‑150 (MQ=255)  acacGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGaa                                                                                                                                    <  2:441605/151‑1 (MQ=255)         gAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCg                                                                                                                             >  1:599396/1‑151 (MQ=255)                           tACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGAcc                                                                                                            <  2:520486/150‑1 (MQ=255)                                   gTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGc                                                                                                   >  1:168081/1‑151 (MQ=255)                                   gTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGc                                                                                                   >  2:6010/1‑151 (MQ=255)                                           tAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCAGCAGCGGGCAGCACATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGACCCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCg                                                                                           >  1:437376/1‑151 (MQ=255)                                                cccGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTcaca                                                                                      <  1:447607/151‑1 (MQ=255)                                                      aGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGcc                                                                                >  1:495528/1‑151 (MQ=255)                                                                aTTGCTGCGCCACGCACGCCCGCAGCGGGTATCATATAGTTGAAGCTTTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAACGGAACCAGCCTGGAAGTGTCAACAGACCGATACGGGCGAGCAGCGTCACAATTGCCAGCACAATAc                                                                      <  2:420759/151‑1 (MQ=255)                                                                aTTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATAc                                                                      <  2:415477/151‑1 (MQ=255)                                                                  tgctgcGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATAcc                                                                     >  2:438401/1‑150 (MQ=255)                                                                          cacgcacgCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTaa                                                            <  2:277233/151‑1 (MQ=255)                                                                           acgcacgcCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAAc                                                           >  1:488205/1‑151 (MQ=255)                                                                             gcacgcCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACAt                                                         >  1:633142/1‑151 (MQ=255)                                                                                cgcCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCa                                                      <  1:628432/151‑1 (MQ=255)                                                                                cgcCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCa                                                      <  2:325455/151‑1 (MQ=255)                                                                                     gCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTAt                                                 >  1:252576/1‑151 (MQ=255)                                                                                          gggTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGt                                            >  1:133524/1‑151 (MQ=255)                                                                                                          aaGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGcc                                >  1:153340/1‑147 (MQ=255)                                                                                                           aGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGAt                            >  1:441422/1‑150 (MQ=255)                                                                                                              tGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAggg                        >  1:519513/1‑151 (MQ=255)                                                                                                                   aGTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCa                   <  1:186119/151‑1 (MQ=255)                                                                                                                    gTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCaa                  <  1:340041/151‑1 (MQ=255)                                                                                                                    gTCCGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCaa                  <  1:189359/151‑1 (MQ=255)                                                                                                                      ccGGCAACCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCaaa                 <  2:133521/150‑1 (MQ=255)                                                                                                                           aaCCCCCGCAACCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCAAAgcgcgc           >  2:164682/1‑151 (MQ=255)                                                                                                                                    aaCCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATGCCAAGACTTAACATCCGTTTATGACGTTTATACAGTGCCAGATAGGGTAGCAAAGCGCGCATTTAAAt   >  1:270721/1‑150 (MQ=255)                                                                                                                                    aaCCGCTGAGGCCGAAAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCAAAGCGCGCATTTAAAtc  >  2:526220/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                        AACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCTGAGGCCGAGAGGAACCAGCCGGAAAGTGTCAACAGACCGATACTGGCGAGCAGCGTCACAATTGCCAGCACAATACCAAGACTTAACATCCATTTATGACGTTTATACAGTGCCAGATAGGGTAGCAAAGCGCGCATTTAAATC  >  NC_000913/928921‑929202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑