| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 96,645 | T→C | V215A (GTG→GCG) | mraY → | phospho‑N‑acetylmuramoyl‑pentapeptide transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 96,645 | 0 | T | C | 97.5% | 135.1 / ‑6.2 | 40 | V215A (GTG→GCG) | mraY | phospho‑N‑acetylmuramoyl‑pentapeptide transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (20/19); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGT > NC_000913/96503‑96789 | gtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTggg < 1:612792/151‑1 (MQ=255) ccGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCg < 1:52551/151‑1 (MQ=255) gCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAAc > 1:575735/1‑151 (MQ=255) tCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGc > 2:134050/1‑151 (MQ=255) aCATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAg < 2:47286/151‑1 (MQ=255) tgctgGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACtt > 1:453905/1‑151 (MQ=255) ggCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCat < 2:558230/151‑1 (MQ=255) ttACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCatat > 1:140426/1‑150 (MQ=255) tCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGt < 1:134053/150‑1 (MQ=255) gtgGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGTCTGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCCGCTACTTGCATATACCGTATCTGCgc < 2:74635/150‑2 (MQ=255) ggTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACg < 1:449308/151‑1 (MQ=255) tGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCggg > 1:259318/1‑151 (MQ=255) tGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCggg > 1:352082/1‑151 (MQ=255) aaCGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAAc > 1:182626/1‑151 (MQ=255) ggTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGtt < 1:615818/151‑1 (MQ=255) gACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGt < 1:134974/151‑1 (MQ=255) aCCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGt > 1:168107/1‑150 (MQ=255) cGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTAcc > 2:354861/1‑151 (MQ=255) tcGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGt > 2:221769/1‑151 (MQ=255) cGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAg < 2:449975/149‑1 (MQ=255) cGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTc > 2:81030/1‑151 (MQ=255) ggCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCgggg < 1:556007/151‑1 (MQ=255) gCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGAc < 2:215105/151‑1 (MQ=255) aaTTATGCCGACCGAATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTg > 2:548361/1‑151 (MQ=255) aTTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTgg > 2:267186/1‑151 (MQ=255) tATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTggg < 2:607945/150‑1 (MQ=255) gCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCtt < 1:544683/150‑1 (MQ=255) cGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCt > 2:193510/1‑151 (MQ=255) cGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCt < 2:188879/151‑1 (MQ=255) cGTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTgg < 1:81030/151‑1 (MQ=255) gTATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGt > 1:369050/1‑151 (MQ=255) tATTTGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATTTGAACTTTGCCAGCTACTTGCAAATCCCGAATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCCCGATAGCCGGGGCAGGACTGGGCTTCCTGGGGtt > 1:404554/1‑151 (MQ=255) ttGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTa > 2:621603/1‑150 (MQ=255) tGTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAac > 2:349361/1‑151 (MQ=255) gTCGCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAac < 2:515593/150‑1 (MQ=255) gCCGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCt < 2:575720/151‑1 (MQ=255) ccGGTGGTTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCt > 1:343333/1‑150 (MQ=255) ggtggtTTTGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATc < 2:596449/151‑1 (MQ=255) tttGCGCTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGgcg < 2:493027/150‑1 (MQ=255) cgcTGGCGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGt > 1:265690/1‑151 (MQ=255) | GTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCCTGTGGTTTAACACCTATCCGGCGCAGGT > NC_000913/96503‑96789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |