| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,124,614 | A→G | V238A (GTG→GCG) | mdtH ← | multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,124,614 | 0 | A | G | 96.8% | 90.5 / ‑3.9 | 31 | V238A (GTG→GCG) | mdtH | multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (14/16); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 | |||||||||||
GCTTAATGACATTATCAACAGCCCAGCCATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTT > NC_000913/1124466‑1124761 | gCTTAATGACATTATCAACAGCCCAGCCATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCcgcg < 1:429111/151‑1 (MQ=255) aaTGACATTATCAACAGCCCAGCCATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGcgtcg < 1:170824/150‑1 (MQ=255) gACATTATCAACAGCCCAGCCATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGa < 1:626610/151‑1 (MQ=255) cagccATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGcagcat < 2:31010/151‑1 (MQ=255) ggTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTg < 1:207336/151‑1 (MQ=255) cAGATGAAAATGCTTTTCACTCCAGCGGTCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGATGACCATAATTGGCAGCATCAGCATCACTTGTAcagcc > 1:441494/1‑151 (MQ=255) cAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTAcagcc > 2:119619/1‑151 (MQ=255) aCGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTAcagccagc > 2:630159/1‑151 (MQ=255) gAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCAt > 2:510482/1‑151 (MQ=255) aaaTGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATgt < 2:591749/151‑1 (MQ=255) aaTGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATgta < 2:176997/151‑1 (MQ=255) tCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCg > 1:131287/1‑151 (MQ=255) cACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGc < 2:236490/151‑1 (MQ=255) ccAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCa < 1:551691/149‑1 (MQ=255) caaTAGGGTAGAGCAACGTTACCGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAAc < 2:355433/149‑1 (MQ=255) gATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAAc > 1:460407/1‑150 (MQ=255) aGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATa < 1:501964/150‑1 (MQ=255) ggTAGAGCAACGTTAACGACAGACCCGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTACCCCGCCAGCGTCAGAACATAGGt < 2:281799/151‑1 (MQ=255) gagCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGAc < 1:512182/150‑1 (MQ=255) aTGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTc > 2:603449/1‑150 (MQ=255) ggCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCAt < 1:107871/150‑1 (MQ=255) aTACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCt > 2:389713/1‑151 (MQ=255) tACATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCtt > 2:337303/1‑151 (MQ=255) aCATCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTc < 1:246984/151‑1 (MQ=255) aTCCATTTAACGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTcgc > 2:211802/1‑151 (MQ=255) aCGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGgcg > 2:369552/1‑151 (MQ=255) cGGCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGgcg < 1:57525/150‑1 (MQ=255) gCAGAGGGCGCGCCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGgcgtgc > 1:531014/1‑151 (MQ=255) gggCGCGCGAGCCGCGTCGTTGACCATAATGGGCAGCATCAGCGTCATTTGTACAGCAAGCATGTTGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTAc < 2:600559/150‑1 (MQ=255) gcgcgcCAGCCGCGTCGTTGAACATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGt < 1:609831/151‑1 (MQ=255) gcgcCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGAAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACCGGCGTTCGTACtgtg > 2:135963/1‑150 (MQ=255) cgcCAGCCGCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTgg > 2:606887/1‑150 (MQ=255) agccgCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGt > 2:232622/1‑151 (MQ=255) agccgCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGt > 2:441343/1‑151 (MQ=255) gccaCGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGtt > 2:577854/1‑151 (MQ=255) | GCTTAATGACATTATCAACAGCCCAGCCATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTT > NC_000913/1124466‑1124761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |