Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,579	C→T	A320V (GCA→GTA)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,579	0	C	T	100.0%	109.3 / NA	30	A320V (GCA→GTA)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

GTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTG  >  NC_000913/1148432‑1148726                                                                                                                                                    |                                                                                                                                                    gTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAg                                                                                                                                                   >  2:217751/1‑150 (MQ=255)         cAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCgg                                                                                                                                          <  1:429880/151‑1 (MQ=255)             agagCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGc                                                                                                                                       >  1:402995/1‑150 (MQ=255)                    gACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACa                                                                                                                                >  1:306437/1‑150 (MQ=255)                     aCGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACa                                                                                                                                >  1:286430/1‑149 (MQ=255)                       gAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGtt                                                                                                                            <  2:124922/151‑1 (MQ=255)                        aGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTc                                                                                                                           >  2:248218/1‑151 (MQ=255)                         ggCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTcc                                                                                                                          <  2:194204/151‑1 (MQ=255)                             aTTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAg                                                                                                                      <  2:211411/151‑1 (MQ=255)                               tCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGc                                                                                                                     <  1:522084/150‑1 (MQ=255)                                cAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCg                                                                                                                    >  2:200829/1‑150 (MQ=255)                                                         aaCGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGta                                                                                           >  1:576967/1‑150 (MQ=255)                                                            ggCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATAcc                                                                                       >  1:541671/1‑151 (MQ=255)                                                              cgcCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCa                                                                                     <  2:442567/151‑1 (MQ=255)                                                                    tctgtTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTgg                                                                                >  2:484252/1‑150 (MQ=255)                                                                          aGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGtttt                                                                            >  2:9919/1‑148 (MQ=255)                                                                            gATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAg                                                                         <  2:205734/149‑1 (MQ=255)                                                                                 cgcgGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTgg                                                                  >  1:130555/1‑151 (MQ=255)                                                                                    ggCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACg                                                               <  1:379152/151‑1 (MQ=255)                                                                                     gCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACgg                                                              <  2:464066/151‑1 (MQ=255)                                                                                        cGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTgg                                                           <  2:324948/151‑1 (MQ=255)                                                                                           tGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTgg                                                           >  1:226103/1‑148 (MQ=255)                                                                                           tGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCa                                                         >  2:319263/1‑150 (MQ=255)                                                                                                  aaGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGa                                                 <  1:403916/151‑1 (MQ=255)                                                                                                      cATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAActc                                             <  1:137124/151‑1 (MQ=255)                                                                                                                     cAGCGAGCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACg                              >  1:525602/1‑151 (MQ=255)                                                                                                                           gCTTTTGCGGTCGCGATTGAACAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGcc                         >  1:266339/1‑150 (MQ=255)                                                                                                                                              aaCAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGc       <  1:511416/149‑1 (MQ=255)                                                                                                                                               aCAGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg    <  1:319270/151‑1 (MQ=255)                                                                                                                                                 aGGTAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTg  <  2:259043/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTTGGCTACAAAAGAGCCTGACGAGGCGATTCAGTCACCTCAACCCCGACCAGTATAACGGCGCCTGTCTGTTAGGATTGCGCGGCACGGTGATAAAAAGTCATGGTGCAGCCAATCAGCGAGCTTTTGCGGTCGCGATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTG  >  NC_000913/1148432‑1148726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑