Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,169,759 C→T D206N (GAT→AAT)  ldtC ← L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,7590CT96.2% 189.4 / ‑2.0 52D206N (GAT→AAT) ldtCL,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (25/25);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCG  >  NC_000913/1169611‑1169902
                                                                                                                                                    |                                                                                                                                               
cgcgCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTgg                                                                                                                                               <  1:392935/151‑1 (MQ=255)
  cgcACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCg                                                                                                                                             >  2:436079/1‑151 (MQ=255)
   gcACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGt                                                                                                                                            <  2:17605/151‑1 (MQ=255)
     aCCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTc                                                                                                                                          >  2:13267/1‑151 (MQ=255)
     aCCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTc                                                                                                                                          <  2:151077/151‑1 (MQ=255)
             gTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATg                                                                                                                                  <  1:13267/151‑1 (MQ=255)
             gTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATg                                                                                                                                  <  1:12824/151‑1 (MQ=255)
                    agagACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCaaa                                                                                                                           <  1:184877/151‑1 (MQ=255)
                         cTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAc                                                                                                                      >  2:423753/1‑151 (MQ=255)
                           tttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAcgc                                                                                                                    >  2:592825/1‑151 (MQ=255)
                           tttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAcg                                                                                                                     <  2:629916/150‑1 (MQ=255)
                           tttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAc                                                                                                                      <  1:414997/149‑1 (MQ=255)
                           tttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAc                                                                                                                      <  1:437366/149‑1 (MQ=255)
                            ttATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAcgcc                                                                                                                   >  2:193922/1‑151 (MQ=255)
                              aTCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAcgccgc                                                                                                                 >  1:62934/1‑151 (MQ=255)
                              aTCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAAcgccg                                                                                                                  <  1:262568/150‑1 (MQ=255)
                                          atgatATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCa                                                                                                     <  2:515536/151‑1 (MQ=255)
                                            gatATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAg                                                                                                    <  1:368286/150‑1 (MQ=255)
                                                        ggTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCatga                                                                                       <  2:77738/151‑1 (MQ=255)
                                                            ccTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGc                                                                                   >  1:357276/1‑151 (MQ=255)
                                                              tGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATTATGGccc                                                                                 >  1:208968/1‑151 (MQ=255)
                                                               ggggTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGcc                                                                                  >  2:590125/1‑149 (MQ=255)
                                                               ggggTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCa                                                                                <  2:630908/151‑1 (MQ=255)
                                                                  gTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTg                                                                             >  1:392071/1‑151 (MQ=255)
                                                                    gACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTggg                                                                           >  2:46953/1‑151 (MQ=255)
                                                                       cTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGtt                                                                         >  1:441357/1‑150 (MQ=255)
                                                                                 agTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAtccagtccag                                                              <  2:433443/151‑1 (MQ=255)
                                                                                            atCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTAcc                                                   >  2:560935/1‑151 (MQ=255)
                                                                                            atCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTAcc                                                   <  2:392064/151‑1 (MQ=255)
                                                                                             tCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTAcc                                                   <  2:83968/150‑1 (MQ=255)
                                                                                                 cATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAACCGGCACTACCGCAgg                                              <  1:32693/151‑1 (MQ=255)
                                                                                                    cccGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCa                                            >  2:369918/1‑150 (MQ=255)
                                                                                                     ccGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAAc                                          >  2:571801/1‑151 (MQ=255)
                                                                                                      cGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACt                                         <  2:396781/151‑1 (MQ=255)
                                                                                                       gCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGACTCGCATGATGGCCCATTGTGTTACCCAGTCCAGCCGGCACTACCGCAgg                                              <  1:22260/145‑1 (MQ=255)
                                                                                                       gCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAACAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTc                                        <  2:501154/151‑1 (MQ=255)
                                                                                                         aGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCa                                       >  2:210974/1‑150 (MQ=255)
                                                                                                           aCGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAAtt                                    >  2:369596/1‑151 (MQ=255)
                                                                                                           aCGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAAtt                                    <  1:321960/151‑1 (MQ=255)
                                                                                                              aaTACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTcc                                  <  2:629644/150‑1 (MQ=255)
                                                                                                               aTACAGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCt                                >  1:594425/1‑151 (MQ=255)
                                                                                                                   aGCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGc                            <  1:443327/151‑1 (MQ=255)
                                                                                                                    gCCAGAACTTACCCGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCt                           >  1:574289/1‑151 (MQ=255)
                                                                                                                                ccGCATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGgcg               <  2:314517/151‑1 (MQ=255)
                                                                                                                                   cATGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg            <  2:69379/151‑1 (MQ=255)
                                                                                                                                    aTGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg            >  2:548345/1‑150 (MQ=255)
                                                                                                                                    aTGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg            >  2:502555/1‑150 (MQ=255)
                                                                                                                                    aTGCCAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg            >  2:66976/1‑150 (MQ=255)
                                                                                                                                        cAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATg         >  1:375568/1‑149 (MQ=255)
                                                                                                                                        cAATGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGtt       >  2:21379/1‑151 (MQ=255)
                                                                                                                                         aaTGCCGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGttt      >  1:627470/1‑151 (MQ=255)
                                                                                                                                             ccGAAATTGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCg  <  2:193941/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCG  >  NC_000913/1169611‑1169902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑