Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	143,721	T→C	V7A (GTG→GCG)	yadH →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	143,721	0	T	C	100.0%	139.6 / NA	40	V7A (GTG→GCG)	yadH	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

GCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCT  >  NC_000913/143573‑143867                                                                                                                                                     |                                                                                                                                                   gCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCgg                                                                                                                                                  <  2:552633/151‑1 (MQ=255)      ggATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTa                                                                                                                                             <  2:196614/151‑1 (MQ=255)       gATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTaa                                                                                                                                            <  2:594336/151‑1 (MQ=255)        aTCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTaaa                                                                                                                                           <  1:102884/151‑1 (MQ=255)         tCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTaaa                                                                                                                                           >  2:607780/1‑150 (MQ=255)           aaCAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAg                                                                                                                                        <  1:496486/151‑1 (MQ=255)             cAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCa                                                                                                                                      <  1:153665/151‑1 (MQ=255)                cGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCt                                                                                                                                   <  1:149479/151‑1 (MQ=255)                  tATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTgg                                                                                                                                 >  2:582411/1‑151 (MQ=255)                        cGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGaaa                                                                                                                           <  2:230358/151‑1 (MQ=255)                              tAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAag                                                                                                                          >  1:124281/1‑146 (MQ=255)                               aaGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATc                                                                                                                     <  2:215461/150‑1 (MQ=255)                                  tGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATc                                                                                                                 >  1:122938/1‑151 (MQ=255)                                    aGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGc                                                                                                               <  1:54235/151‑1 (MQ=255)                                      cAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCtt                                                                                                             <  2:6278/151‑1 (MQ=255)                                       aGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCttt                                                                                                            <  2:391097/151‑1 (MQ=255)                                           cATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGc                                                                                                        <  1:573324/151‑1 (MQ=255)                                           cATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGc                                                                                                        >  2:572902/1‑151 (MQ=255)                                                    aTTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTggg                                                                                               >  1:600407/1‑151 (MQ=255)                                                            tGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACg                                                                                       >  2:353503/1‑151 (MQ=255)                                                                           aCCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGtc                                                                        >  2:565791/1‑151 (MQ=255)                                                                                            tttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCcttt                                                        >  1:223726/1‑150 (MQ=255)                                                                                             ttgtttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCcttta                                                       <  1:572914/150‑1 (MQ=255)                                                                                                 ttCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCctttacttt                                                   >  2:85720/1‑150 (MQ=255)                                                                                                   cACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTttatt                                                >  1:415672/1‑151 (MQ=255)                                                                                                               aaaaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAAc                                    >  2:8968/1‑151 (MQ=255)                                                                                                                 aaaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCt                                  >  2:196649/1‑151 (MQ=255)                                                                                                                  aaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACTATGACCCTTTACTTTATTATCTTCGGTAACCTg                                 >  2:628473/1‑151 (MQ=255)                                                                                                                  aaCAAGGAGATCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTg                                 <  1:450823/151‑1 (MQ=255)                                                                                                                        gagaCCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCGCCATGTCCCTTTACTTTATTATCTTCGGTAACCTGATTgg                            <  1:8968/150‑1 (MQ=255)                                                                                                                           aTCGCGCATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTcg                        >  2:46359/1‑151 (MQ=255)                                                                                                                                gcATGATGCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTAt                   >  1:491328/1‑151 (MQ=255)                                                                                                                                     atgCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGc               <  1:435073/150‑1 (MQ=255)                                                                                                                                     atgCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCg              <  1:73585/151‑1 (MQ=255)                                                                                                                                      tgCATCTTTACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCg              >  2:630677/1‑150 (MQ=255)                                                                                                                                         aTCTTTACTGGGCGGCGCTAAAAAGCATCT‑GGCGAAAGAGATCCCTCCCTTTCTGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGCCATCACCATGACCCTTTACTTTATTATCTTCGGGCACCCGATTGGTTTGCGTATTGGCGatat          >  2:144163/1‑150 (MQ=255)                                                                                                                                             ttACTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCa       <  1:353168/150‑1 (MQ=255)                                                                                                                                               aCTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATgg    >  2:275866/1‑151 (MQ=255)                                                                                                                                                cTGGGCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGc   >  1:327457/1‑151 (MQ=255)                                                                                                                                                  gggCGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCt  <  1:375966/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGAAAAACAAGGAGATCGCGCATGATGCATCTTTACTGGGTGGCGCTAAAAAGCATCTGGGCGAAAGAGATCCATCGCTTTATGCGTATCTGGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCT  >  NC_000913/143573‑143867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑