Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,314,744 T→C T161A (ACT→GCT)  yciE ← putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,7440TC98.0% 189.5 / ‑4.0 51T161A (ACT→GCT) yciEputative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (27/23);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTG  >  NC_000913/1314596‑1314887
                                                                                                                                                    |                                                                                                                                               
cAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGctt                                                                                                                                               >  2:196471/1‑151 (MQ=255)
     cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag                                                                                                                                            >  2:562439/1‑149 (MQ=255)
       aTAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCg                                                                                                                                        <  2:383033/151‑1 (MQ=255)
            gTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAAtt                                                                                                                                    >  1:150481/1‑150 (MQ=255)
                 ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAAt                                                                                                                              >  1:631050/1‑151 (MQ=255)
                        ccGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCa                                                                                                                        <  2:360593/150‑1 (MQ=255)
                        ccGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCa                                                                                                                        <  1:230971/150‑1 (MQ=255)
                           tGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAgttg                                                                                                                    >  2:131214/1‑151 (MQ=255)
                                    aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGa                                                                                                            >  1:50993/1‑150 (MQ=255)
                                        tctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATAtt                                                                                                       >  1:179202/1‑151 (MQ=255)
                                           cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCt                                                                                                     <  2:231859/150‑1 (MQ=255)
                                           cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTg                                                                                                    >  2:340732/1‑151 (MQ=255)
                                            agcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGa                                                                                                   <  1:364402/151‑1 (MQ=255)
                                                  atatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGc                                                                                             >  2:413919/1‑151 (MQ=255)
                                                      cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAg                                                                                          <  1:186182/150‑1 (MQ=255)
                                                      cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAg                                                                                          <  2:602153/150‑1 (MQ=255)
                                                      cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAg                                                                                          <  1:462393/150‑1 (MQ=255)
                                                      cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGt                                                                                         >  2:35888/1‑151 (MQ=255)
                                                       gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                                                                               <  2:140808/128‑1 (MQ=255)
                                                       gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                                                                               >  1:140811/1‑128 (MQ=255)
                                                           ttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGc                                                                                     <  2:602644/150‑1 (MQ=255)
                                                              aTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAAGCAGCCAGTCGGCCAtt                                                                                 <  2:205144/151‑1 (MQ=255)
                                                                 ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGc                                                                              >  2:607938/1‑151 (MQ=255)
                                                                    cacaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                                                                           >  2:21112/1‑151 (MQ=255)
                                                                         aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTc                                                                      >  1:397949/1‑151 (MQ=255)
                                                                         aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTc                                                                      >  2:611395/1‑151 (MQ=255)
                                                                           aCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt                                                                    >  1:424250/1‑151 (MQ=255)
                                                                             gACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAttt                                                                  >  1:238456/1‑151 (MQ=255)
                                                                                     aaTATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGc                                                          >  1:197378/1‑151 (MQ=255)
                                                                                          aCGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCg                                                      >  1:289324/1‑150 (MQ=255)
                                                                                              ggTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGt                                                 <  2:626654/151‑1 (MQ=255)
                                                                                              ggTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGt                                                 <  1:590842/151‑1 (MQ=255)
                                                                                                tCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTg                                               >  1:590526/1‑151 (MQ=255)
                                                                                                      aGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTg                                         <  1:377171/151‑1 (MQ=255)
                                                                                                       ggACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGa                                        <  2:556873/151‑1 (MQ=255)
                                                                                                        gACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGa                                        >  1:370424/1‑150 (MQ=255)
                                                                                                                ataAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTAt                                <  1:9120/150‑1 (MQ=255)
                                                                                                                   aaGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCAcc                            >  2:103432/1‑151 (MQ=255)
                                                                                                                   aaGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCAcc                            <  1:219993/151‑1 (MQ=255)
                                                                                                                    aGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCg                           <  1:63707/151‑1 (MQ=255)
                                                                                                                         attattTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtt                      >  2:24408/1‑151 (MQ=255)
                                                                                                                         attattTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAt                       <  2:113299/150‑1 (MQ=255)
                                                                                                                              ttcttcGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtttttt                  <  2:346363/150‑1 (MQ=255)
                                                                                                                               tcttcGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTtgc                >  1:1585/1‑151 (MQ=255)
                                                                                                                                 ttcGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTtgct               >  1:456592/1‑150 (MQ=255)
                                                                                                                                      ttCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTgctgctgct         >  2:6461/1‑151 (MQ=255)
                                                                                                                                       tCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTgctgctgct         >  1:440864/1‑150 (MQ=255)
                                                                                                                                       tCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTgctgctgct         >  1:420381/1‑150 (MQ=255)
                                                                                                                                          aCGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTa        <  2:353279/148‑1 (MQ=255)
                                                                                                                                          aCGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAt      <  2:451375/150‑1 (MQ=255)
                                                                                                                                             ccATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTg  <  1:610155/151‑1 (MQ=255)
                                                                                                                                             ccATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTg  >  1:210441/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTG  >  NC_000913/1314596‑1314887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑