| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,321,083 | G→A | A101V (GCG→GTG) | trpD ← | fused glutamine amidotransferase (component II) of anthranilate synthase/anthranilate phosphoribosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,321,083 | 0 | G | A | 94.1% | 122.5 / ‑6.1 | 34 | A101V (GCG→GTG) | trpD | fused glutamine amidotransferase (component II) of anthranilate synthase/anthranilate phosphoribosyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/20); minor base C (0/2); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGCCAGG > NC_000913/1320939‑1321219 | tGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGAc > 2:253887/1‑151 (MQ=255) gATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACAt > 2:597431/1‑149 (MQ=255) tAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGccccc < 1:420704/150‑1 (MQ=255) aaaCCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGt < 1:330550/151‑1 (MQ=255) cggccggAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGc > 1:411858/1‑151 (MQ=255) gccggAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCtt < 2:233408/151‑1 (MQ=255) ccggAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTc < 1:297188/151‑1 (MQ=255) ttACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCg > 1:582552/1‑151 (MQ=255) gCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTg < 2:137135/150‑1 (MQ=255) gCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTg > 2:130227/1‑150 (MQ=255) aaCCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCTGTAAGCTTCGACAATCGCCTGATGt > 1:516443/1‑151 (MQ=255) aGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGa < 1:251290/151‑1 (MQ=255) gCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCg > 1:498820/1‑149 (MQ=255) gATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCaaa < 2:546861/150‑1 (MQ=255) aaCGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTGAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGcc < 1:148039/151‑1 (MQ=255) ccACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCTGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCaataat < 2:516432/151‑1 (MQ=255) aCCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATgg > 2:196408/1‑151 (MQ=255) cGGCAGCGGGTTTGTTAATCCGGGAAAGATCGCGTGGCCGGCATGGTCAATGGTGGAGGCTTTAGCGTGGAGAATTTCGCCCCCCTGACCGGCAAAGCGTACGGAAGCGTAGTCAAGCGCCTGATGTCGGAGGCAAATGGCAATAATgg < 2:564569/149‑1 (MQ=255) aGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAg > 2:95690/1‑128 (MQ=255) aGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAg < 1:95690/128‑1 (MQ=255) tGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACg < 2:411851/150‑1 (MQ=255) tGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACg < 2:498810/150‑1 (MQ=255) aaTCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAg < 2:625297/150‑1 (MQ=255) aaTCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGc > 1:28662/1‑151 (MQ=255) ccGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCgg < 1:384945/150‑1 (MQ=255) cGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGt < 2:492966/151‑1 (MQ=255) cAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCCCCTGACCGACATAGCCCCCGTAAGCTTCGCCAATCGCCTGATCTCCGAGGCAAATGCCAACCATGGGCAGCTTGCCACGCAAGCGGGTgag < 2:557669/151‑1 (MQ=255) aTCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGtt < 2:110944/151‑1 (MQ=255) cGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTcc < 2:229171/151‑1 (MQ=255) gACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCAt > 2:506631/1‑151 (MQ=255) cATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAAcc < 2:90726/151‑1 (MQ=255) gCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTc < 2:564780/151‑1 (MQ=255) cGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCTACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGcc < 2:253510/151‑1 (MQ=255) gagaATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGCCAgg > 1:93279/1‑150 (MQ=255) | TGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGCCAGG > NC_000913/1320939‑1321219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |