Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,370,155	A→G	intergenic (+152/‑61)	pspE → / → ycjM	thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,370,155	0	A	G	100.0%	176.7 / NA	47	intergenic (+152/‑61)	pspE/ycjM	thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/20);  total (27/20)

AATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCT  >  NC_000913/1370007‑1370301                                                                                                                                                     |                                                                                                                                                   aaTTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCa                                                                                                                                                  <  2:492348/151‑1 (MQ=255) aaTTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCa                                                                                                                                                  <  2:194185/151‑1 (MQ=255)     cACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGttt                                                                                                                                              <  2:95755/151‑1 (MQ=255)        cATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTAtt                                                                                                                                           >  2:417857/1‑151 (MQ=255)            aGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCt                                                                                                                                       <  1:143170/151‑1 (MQ=255)             gcggcgCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCt                                                                                                                                       >  2:594262/1‑150 (MQ=255)                         cgcCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGtctc                                                                                                                          >  1:139739/1‑151 (MQ=255)                            cGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCg                                                                                                                       <  1:571279/151‑1 (MQ=255)                             gTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGt                                                                                                                      <  2:555116/151‑1 (MQ=255)                                       cTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAc                                                                                                            >  1:125736/1‑151 (MQ=255)                                         gTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAc                                                                                                            <  1:593801/149‑1 (MQ=255)                                          tCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGc                                                                                                         >  1:528014/1‑151 (MQ=255)                                              acCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTAcc                                                                                                     >  2:50270/1‑151 (MQ=255)                                              acCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTAcc                                                                                                     >  2:460575/1‑151 (MQ=255)                                               ccTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCa                                                                                                    <  1:530977/151‑1 (MQ=255)                                                  tATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACg                                                                                                  <  2:171698/150‑1 (MQ=255)                                                            cccATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAAc                                                                                        >  2:76619/1‑150 (MQ=255)                                                             ccATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACAt                                                                                      >  2:155052/1‑151 (MQ=255)                                                              cATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                                                                     >  2:367504/1‑151 (MQ=255)                                                                 aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCATGGAAAGGCAACATGaa                                                                                   <  2:624213/150‑1 (MQ=255)                                                                 aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGaa                                                                                   <  1:415978/150‑1 (MQ=255)                                                                  gATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAAc                                                                                 <  2:26489/151‑1 (MQ=255)                                                                         tGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGaaaa                                                                           <  1:194876/150‑1 (MQ=255)                                                                                 cTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGAtt                                                                  >  2:602553/1‑151 (MQ=255)                                                                                  tCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGAtt                                                                  >  1:545484/1‑150 (MQ=255)                                                                                     caAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCt                                                              >  2:190542/1‑151 (MQ=255)                                                                                         tACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGAc                                                          >  2:148781/1‑151 (MQ=255)                                                                                          aCGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACg                                                         <  1:417864/151‑1 (MQ=255)                                                                                               ttttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGaaa                                                      <  1:410368/149‑1 (MQ=255)                                                                                               ttttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAAt                                                     <  2:400211/150‑1 (MQ=255)                                                                                                tttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCt                                                   >  2:466531/1‑151 (MQ=255)                                                                                                      tGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTg                                             >  1:166604/1‑151 (MQ=255)                                                                                                             aaaTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACAtt                                      >  2:483479/1‑151 (MQ=255)                                                                                                              aaTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACAttt                                     >  1:398985/1‑151 (MQ=255)                                                                                                                tGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAc                                   >  1:359012/1‑151 (MQ=255)                                                                                                                tGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAc                                   >  1:557272/1‑151 (MQ=255)                                                                                                                 gATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAcc                                  >  2:552359/1‑151 (MQ=255)                                                                                                                  aTTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAcc                                  <  2:515864/150‑1 (MQ=255)                                                                                                                  aTTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCg                                 >  2:399146/1‑151 (MQ=255)                                                                                                                    ttCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGc                                <  2:506586/150‑1 (MQ=255)                                                                                                                              aCTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTAc                     <  2:250184/151‑1 (MQ=255)                                                                                                                                tAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTAc                     >  2:90687/1‑149 (MQ=255)                                                                                                                                       cTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTg            >  2:433098/1‑151 (MQ=255)                                                                                                                                          gtgCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTaa         <  1:261647/151‑1 (MQ=255)                                                                                                                                          gtgCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTa          >  2:146223/1‑150 (MQ=255)                                                                                                                                             cTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAAcgc      >  1:557317/1‑151 (MQ=255)                                                                                                                                                 aTTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCt  >  2:75430/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCT  >  NC_000913/1370007‑1370301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑