Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,436,536	A→G	Y120H (TAC→CAC)	ompN ←	outer membrane pore protein N, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,436,536	0	A	G	100.0%	116.8 / NA	33	Y120H (TAC→CAC)	ompN	outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

GCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGT  >  NC_000913/1436388‑1436673                                                                                                                                                     |                                                                                                                                          gcgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCa                                                                                                                                         <  2:4215/151‑1 (MQ=255)  cgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAt                                                                                                                                        >  1:200124/1‑151 (MQ=255)  cgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAt                                                                                                                                        >  2:600644/1‑151 (MQ=255)   gAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAtt                                                                                                                                       >  1:49659/1‑151 (MQ=255)     aGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTAc                                                                                                                                     <  2:302634/151‑1 (MQ=255)      gTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACg                                                                                                                                    <  1:86207/151‑1 (MQ=255)          aGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCAt                                                                                                                                >  2:250063/1‑151 (MQ=255)               aTTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTa                                                                                                                           <  1:204852/151‑1 (MQ=255)                  tACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGt                                                                                                                        >  2:263955/1‑151 (MQ=255)                   aCCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGt                                                                                                                        <  2:359450/150‑1 (MQ=255)                   aCCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTc                                                                                                                       <  2:492979/151‑1 (MQ=255)                          cGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATc                                                                                                                <  2:394772/151‑1 (MQ=255)                          cGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAAGCAGAATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTaa                                                                                                                  >  2:162485/1‑149 (MQ=255)                                 aTCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGa                                                                                                          >  2:401288/1‑150 (MQ=255)                                               aaGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCaa                                                                                            <  1:567740/150‑1 (MQ=255)                                                   cgcgACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAAttt                                                                                        <  2:305277/150‑1 (MQ=255)                                                    gcgACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCa                                                                                      <  1:362250/151‑1 (MQ=255)                                                          cgaTTGGCTCGACCAGTCCTCAAGGTGTCTTCATTGGTATAAGAGTCACCGCCAAATTCAGGCTGCATATCGGACCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCa                                                                                      >  2:551583/3‑145 (MQ=255)                                                                   cGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGc                                                                         <  1:500973/149‑1 (MQ=255)                                                                   cGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCa                                                                       <  2:588306/151‑1 (MQ=255)                                                                    gACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAg                                                                      <  2:239856/151‑1 (MQ=255)                                                                             tAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTcc                                                              >  1:22469/1‑150 (MQ=255)                                                                                aGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGa                                                          <  1:6298/151‑1 (MQ=255)                                                                                          aTTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGtttttt                                                >  1:4750/1‑151 (MQ=255)                                                                                            tGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTg                                               <  1:215761/150‑1 (MQ=255)                                                                                                         aCCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAgtgt                                   <  1:301816/149‑1 (MQ=255)                                                                                                            gCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGACGTGCATTACGCCATAATTACGTCCGTAATCGAAATAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATCACTGGTTTTTTGAAGATTCAGTGTTGttt                              <  2:397062/151‑1 (MQ=255)                                                                                                                      ggCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAatat                    >  1:603527/1‑151 (MQ=255)                                                                                                                        cagcaTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTg                  >  2:464379/1‑151 (MQ=255)                                                                                                                           caTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt               >  2:103878/1‑151 (MQ=255)                                                                                                                           caTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt               >  1:357704/1‑151 (MQ=255)                                                                                                                           caTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt               >  1:219730/1‑151 (MQ=255)                                                                                                                                         cTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGt  >  2:123978/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                          GCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGT  >  NC_000913/1436388‑1436673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑