Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,033	A→G	V234A (GTG→GCG)	dosP ←	oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,033	0	A	G	100.0%	139.7 / NA	39	V234A (GTG→GCG)	dosP	oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/19);  total (20/19)

CGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTC  >  NC_000913/1564887‑1565179                                                                                                                                                   |                                                                                                                                                   cGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTc                                                                                                                                                >  2:307173/1‑151 (MQ=255)   aTGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCAt                                                                                                                                              >  1:368720/1‑151 (MQ=255)         cGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCg                                                                                                                                        <  2:521201/151‑1 (MQ=255)         cGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCg                                                                                                                                        <  1:402735/151‑1 (MQ=255)         cGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCg                                                                                                                                        >  2:338546/1‑151 (MQ=255)           aCAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCggg                                                                                                                                      <  2:569933/151‑1 (MQ=255)                    ttCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATag                                                                                                                               >  2:59492/1‑149 (MQ=255)                    ttCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATag                                                                                                                               >  1:53856/1‑149 (MQ=255)                             ttCATGAAATGGCGTGCTGCTGCACATTGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGCTCTTTGGTGAAATCCCCGAAAGTCATTACAAGGTTCTTCAGATGTGCGAGCGCGTCATAAACCGGGCTGATAAAGGCTTTGATc                                                                                                                    >  2:447320/1‑151 (MQ=255)                             ttCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAAt                                                                                                                     <  2:202473/150‑1 (MQ=255)                               cATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATcc                                                                                                                   >  2:497063/1‑150 (MQ=255)                                      tGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGAttttt                                                                                                           >  2:546482/1‑151 (MQ=255)                                       ggCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGAtttttt                                                                                                          <  2:300282/151‑1 (MQ=255)                                       ggCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGAtttttt                                                                                                          <  2:272417/151‑1 (MQ=255)                                         cGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTc                                                                                                         >  1:112416/1‑150 (MQ=255)                                            gctgctGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCg                                                                                                     <  1:328491/151‑1 (MQ=255)                                                               agaATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGaa                                                                                  <  2:331173/151‑1 (MQ=255)                                                               agaATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGa                                                                                   >  2:604785/1‑150 (MQ=255)                                                               agaATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGa                                                                                   >  1:421711/1‑150 (MQ=255)                                                                       gCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTc                                                                           <  1:110425/150‑1 (MQ=255)                                                                        ccTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCt                                                                         >  1:9586/1‑151 (MQ=255)                                                                                    cGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCgcgggcgg                                                             <  1:419524/151‑1 (MQ=255)                                                                                     gAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCCTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCgcgggcgg                                                             >  2:507887/1‑150 (MQ=255)                                                                                       aTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGttt                                                          >  1:379155/1‑151 (MQ=255)                                                                                           gCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCa                                                      <  1:576527/151‑1 (MQ=255)                                                                                            ccGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCAt                                                     <  2:308430/151‑1 (MQ=255)                                                                                                   tcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAAc                                               <  1:259855/150‑1 (MQ=255)                                                                                                        gATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtgttgt                                         >  2:147448/1‑151 (MQ=255)                                                                                                        gATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtgttgt                                         <  1:307180/151‑1 (MQ=255)                                                                                                             ccGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACg                                    >  2:50163/1‑151 (MQ=255)                                                                                                                 gaAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                                <  2:198143/151‑1 (MQ=255)                                                                                                                  aaaGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGc                               <  1:211998/151‑1 (MQ=255)                                                                                                                       cATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGtt                           >  1:480290/1‑150 (MQ=255)                                                                                                                             cAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCa                    <  1:546493/151‑1 (MQ=255)                                                                                                                                   cTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAAt              >  2:336827/1‑151 (MQ=255)                                                                                                                                       aGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAg          <  1:563398/151‑1 (MQ=255)                                                                                                                                           gcgcGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAAt      <  1:416169/151‑1 (MQ=255)                                                                                                                                               gAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGtt   >  1:592755/1‑150 (MQ=255)                                                                                                                                                aGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTc  >  2:306461/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTC  >  NC_000913/1564887‑1565179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑