Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,772,863	C→T	L118L (CTG→TTG)	ydiN →	putative MFS transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,772,863	0	C	T	100.0%	135.9 / NA	36	L118L (CTG→TTG)	ydiN	putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

GTGATCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTA  >  NC_000913/1772719‑1773008                                                                                                                                                 |                                                                                                                                                  gtgATCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATa                                                                                                                                             <  2:158182/151‑1 (MQ=255)    aTCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACgg                                                                                                                                          >  1:330685/1‑151 (MQ=255)       tcCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTg                                                                                                                                       >  1:6187/1‑151 (MQ=255)        ccGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACgg                                                                                                                                          <  2:519128/147‑1 (MQ=255)           aTAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTa                                                                                                                                   <  2:204314/151‑1 (MQ=255)              aGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTAccc                                                                                                                                >  2:2874/1‑151 (MQ=255)                   ggTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCcgcg                                                                                                                            <  2:174245/150‑1 (MQ=255)                           ggCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGaa                                                                                                                   <  1:568176/151‑1 (MQ=255)                            gCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAAt                                                                                                                  >  2:191084/1‑151 (MQ=255)                             cGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATg                                                                                                                 >  1:426936/1‑151 (MQ=255)                                             aGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCctct                                                                                                 >  1:136943/1‑151 (MQ=255)                                              gTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTg                                                                                                <  2:301715/151‑1 (MQ=255)                                              gTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTg                                                                                                >  1:6934/1‑151 (MQ=255)                                               taataaTGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATCTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTgg                                                                                               >  1:69157/1‑151 (MQ=255)                                                 ataatGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGtt                                                                                             <  1:48624/151‑1 (MQ=255)                                                             cTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATAc                                                                                 >  2:24324/1‑151 (MQ=255)                                                                              tCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGgtg                                                                >  1:258113/1‑151 (MQ=255)                                                                                      gCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTAGTTCGGCGGTCATACTGGTTAAATCGATGGTGTCATTTgg                                                        <  2:198044/151‑1 (MQ=255)                                                                                         cGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTggg                                                       >  1:488258/1‑149 (MQ=255)                                                                                           aaTTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCaaa                                                   <  2:126212/151‑1 (MQ=255)                                                                                             tttAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATg                                                 >  2:219304/1‑151 (MQ=255)                                                                                                aaCTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGtt                                               <  2:628488/150‑1 (MQ=255)                                                                                                      cGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCAAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTAccc                                         >  2:419281/1‑150 (MQ=255)                                                                                                       gCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCaa                                       <  1:567835/151‑1 (MQ=255)                                                                                                        ccTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAAt                                      <  1:323313/151‑1 (MQ=255)                                                                                                           aCGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCt                                   <  1:376168/151‑1 (MQ=255)                                                                                                           aCGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCt                                   <  1:624594/151‑1 (MQ=255)                                                                                                           aCGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATAATGGTTAAAGCGATGGGGTCATTTGGGCAAATGTTCTACCCAATGc                                    >  1:141851/1‑150 (MQ=255)                                                                                                                  ggCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGa                              <  2:36683/149‑1 (MQ=255)                                                                                                                     aGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCta                          >  1:129611/1‑150 (MQ=255)                                                                                                                        gtgCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGGTAAAGCGATGGTGTCATTTGGGCAAATGGTCTACCCAATGCTGGTGAGCTATATg                      >  1:565552/1‑151 (MQ=255)                                                                                                                           cGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTg                   <  2:258106/151‑1 (MQ=255)                                                                                                                                   tCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCaata            >  1:585875/1‑150 (MQ=255)                                                                                                                                         aCTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTg     <  2:604188/151‑1 (MQ=255)                                                                                                                                            cAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGt   <  1:611737/150‑1 (MQ=255)                                                                                                                                            cAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTa  <  1:492598/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GTGATCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTA  >  NC_000913/1772719‑1773008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑