Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,823,902	T→C	Y130H (TAT→CAT)	cho →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,823,902	0	T	C	100.0%	139.8 / NA	37	Y130H (TAT→CAT)	cho	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

ATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGC  >  NC_000913/1823761‑1824045                                                                                                                                              |                                                                                                                                                atatGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAag                                                                                                                                        <  1:406641/151‑1 (MQ=255)  tatGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCaa                                                                                                                                          >  1:128949/1‑148 (MQ=255)    tGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAaga                                                                                                                                       <  2:104369/149‑1 (MQ=255)      tACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAagag                                                                                                                                      <  2:626949/148‑1 (MQ=255)            cgGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGAtttt                                                                                                                             <  2:79078/151‑1 (MQ=255)              gTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTc                                                                                                                           <  1:609826/151‑1 (MQ=255)                  aaTCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCAc                                                                                                                         >  1:111587/1‑149 (MQ=255)                            ctGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATTAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAAc                                                                                                              >  2:232900/1‑150 (MQ=255)                              gCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTg                                                                                                           <  2:300151/151‑1 (MQ=255)                                         cgcgATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGtt                                                                                                >  2:478223/1‑151 (MQ=255)                                                aaTCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGCGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCaa                                                                                          >  1:516865/1‑150 (MQ=255)                                                aaTCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCaa                                                                                          >  2:111275/1‑150 (MQ=255)                                                   cAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAgg                                                                                      <  1:279987/151‑1 (MQ=255)                                                    aaaGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGgc                                                                                     <  2:300004/151‑1 (MQ=255)                                                        aacaacAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGgcgcgc                                                                                 >  2:547985/1‑151 (MQ=255)                                                         acaacaGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCa                                                                                <  2:293468/151‑1 (MQ=255)                                                         acaacaGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTAGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGgcgcgc                                                                                 >  1:225584/1‑150 (MQ=255)                                                             caGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCtt                                                                            <  2:57834/151‑1 (MQ=255)                                                                        taataaACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCAtt                                                                  <  1:45340/150‑1 (MQ=255)                                                                            aaaCGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGa                                                             <  1:602232/151‑1 (MQ=255)                                                                                   tGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGc                                                      >  2:81465/1‑151 (MQ=255)                                                                                      gccgcAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCtga                                                   <  2:622203/151‑1 (MQ=255)                                                                                         gcAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCtgatg                                                 >  2:390034/1‑150 (MQ=255)                                                                                          cAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCtgatga                                                >  1:389341/1‑150 (MQ=255)                                                                                                  aGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACt                                       <  2:88303/151‑1 (MQ=255)                                                                                                    ctctGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACttt                                     >  1:306920/1‑151 (MQ=255)                                                                                                          gCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATg                               >  2:597650/1‑151 (MQ=255)                                                                                                          gCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATg                               >  2:630108/1‑151 (MQ=255)                                                                                                                cAATTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctgc                         <  1:269806/151‑1 (MQ=255)                                                                                                                  aTTAAATGAAAAGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctgctg                       <  1:315169/151‑1 (MQ=255)                                                                                                                          aaaaGCGCGTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTgg                >  1:597249/1‑150 (MQ=255)                                                                                                                                 cgTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGtt         >  2:468225/1‑150 (MQ=255)                                                                                                                                 cgTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGtt         >  2:156255/1‑150 (MQ=255)                                                                                                                                  gTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTaa       <  1:237707/151‑1 (MQ=255)                                                                                                                                  gTCGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTa        >  1:492842/1‑150 (MQ=255)                                                                                                                                    cGATGTGGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGt     >  2:317871/1‑151 (MQ=255)                                                                                                                                       tgtgGTGCATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTcgc  >  2:73146/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                ATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGC  >  NC_000913/1823761‑1824045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑