Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,883,282	T→C	L32P (CTC→CCC)	yeaV →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,883,282	0	T	C	100.0%	108.9 / NA	33	L32P (CTC→CCC)	yeaV	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

TTATGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCTCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTCCCTACTC  >  NC_000913/1883135‑1883429                                                                                                                                                    |                                                                                                                                                    ttATGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCtctc                                                                                                                                                  >  2:225630/1‑151 (MQ=255)  tATGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTcc                                                                                                                                                 >  2:366791/1‑151 (MQ=255)   aTGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCcctcct                                                                                                                                                >  2:253145/1‑151 (MQ=255)          aTGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtt                                                                                                                                         >  1:431957/1‑151 (MQ=255)            gCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgttt                                                                                                                                        <  1:463241/150‑1 (MQ=255)              aaaTTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtttatg                                                                                                                                     >  1:145216/1‑151 (MQ=255)                   ttGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTgtttatgtttat                                                                                                                                >  1:240115/1‑151 (MQ=255)                     gTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTAttt                                                                                                                              <  1:241608/151‑1 (MQ=255)                                gTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGttt                                                                                                                    >  1:150934/1‑150 (MQ=255)                                   aGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGt                                                                                                                >  1:589832/1‑151 (MQ=255)                                     gTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGttc                                                                                                              <  2:70590/151‑1 (MQ=255)                                     gTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGtt                                                                                                               >  1:493653/1‑150 (MQ=255)                                         cgccgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTtcttct                                                                                                          >  2:607507/1‑151 (MQ=255)                                         cgccgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTtcttct                                                                                                          <  2:56705/151‑1 (MQ=255)                                            cgTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACg                                                                                                       >  2:566850/1‑151 (MQ=255)                                             gTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtttatgttta                                                                                                                                 >  1:561420/1‑124 (MQ=255)                                             gTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCtgtttatgttta                                                                                                                                 <  2:561408/124‑1 (MQ=255)                                             gTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGc                                                                                                      >  2:293870/1‑151 (MQ=255)                                               aCAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCt                                                                                                     <  1:182841/150‑1 (MQ=255)                                                 aGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTa                                                                                                  <  2:1002/151‑1 (MQ=255)                                                             tGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTg                                                                                       <  2:364923/150‑1 (MQ=255)                                                                 ggCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGa                                                                                   >  2:319716/1‑150 (MQ=255)                                                                 ggCAAAGAGAGTGGTGATTTATATAGCAGCCAGTATATACGGCAATATTCGTCTTGGGGAAAGAAAACCGGAATACAGCACGCCGGCCTGGCTGTTTATGTTTATGGGCGACGGTATAGGTTCTTCGACGCTTTATTGGGGGGTTGCTg                                                                                    <  2:19507/149‑1 (MQ=255)                                                                       gggACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGcc                                                                            >  1:303588/1‑151 (MQ=255)                                                                          aCTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCtat                                                                         <  2:482307/151‑1 (MQ=255)                                                                             ggTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCtattat                                                                      <  2:92828/151‑1 (MQ=255)                                                                                       ttaGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAaca                                                             <  2:235995/150‑1 (MQ=255)                                                                                                aGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTaa                                                   <  1:154550/151‑1 (MQ=255)                                                                                                                ttCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTc                                   >  2:87743/1‑151 (MQ=255)                                                                                                                  cGTCTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTcac                                 >  1:288083/1‑151 (MQ=255)                                                                                                                     cTTGGCGAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTcaca                                <  1:87743/149‑1 (MQ=255)                                                                                                                           gAAGGAAAACCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCAc                        <  2:581138/151‑1 (MQ=255)                                                                                                                                 aaaCCGGAATACAGCACGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAAt                  <  1:118388/151‑1 (MQ=255)                                                                                                                                                aCGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTCCCTAct   >  2:516452/1‑151 (MQ=255)                                                                                                                                                 cGCCCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTCCCTActc  >  2:391722/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TTATGTAACATGCAAATTTTGTTACGCGTACGTTAGGTTCCGCCGTACAGGTATTGGTTTTGCTGGCAATGGGACTGGTGATTTATTTAGCCACCAGTAAATACGGCAATATTCGTCTTGGCGAAGGAAAACCGGAATACAGCACGCTCTCCTGGCTGTTTATGTTTATTTGTGCCGGTTTAGGTTCTTCTACGCTTTATTGGGGGGTTGCTGAATGGGCCTATTATTATCAAACACCTGGATTAAATATCGCACCGCGTTCACAACAGGCACTCGAATTTAGCGTTCCCTACTC  >  NC_000913/1883135‑1883429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑