Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,923,990	A→G	V201A (GTA→GCA)	yebZ ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,923,990	0	A	G	100.0%	114.5 / NA	31	V201A (GTA→GCA)	yebZ	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/12);  total (19/12)

CATAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGA  >  NC_000913/1923853‑1924133                                                                                                                                          |                                                                                                                                                cATAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGc                                                                                                                                    <  1:430650/151‑1 (MQ=255)   tAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCa                                                                                                                                   >  2:465052/1‑150 (MQ=255)    aaCGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCaa                                                                                                                                  <  1:494946/150‑1 (MQ=255)    aaCGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCaa                                                                                                                                  >  2:374784/1‑150 (MQ=255)         ttAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGcc                                                                                                                            >  1:496796/1‑151 (MQ=255)           aGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCg                                                                                                                          <  2:310804/151‑1 (MQ=255)                 cgcAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAGTCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAg                                                                                                                    <  2:566774/151‑1 (MQ=255)                                aaTCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAgc                                                                                                     >  1:126332/1‑151 (MQ=255)                                   catcaCCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGcc                                                                                                  <  1:230071/151‑1 (MQ=255)                                          aGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAAt                                                                                             >  1:297096/1‑149 (MQ=255)                                          aGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAg                                                                                           <  1:619347/151‑1 (MQ=255)                                             aTGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCt                                                                                        >  1:513744/1‑151 (MQ=255)                                              tGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAACGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTg                                                                                       >  1:312426/1‑151 (MQ=255)                                                    tGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGgtg                                                                                    <  2:423844/148‑1 (MQ=255)                                                    tGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTc                                                                                  <  1:593804/150‑1 (MQ=255)                                                    tGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCg                                                                                 >  1:200155/1‑151 (MQ=255)                                                      aGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGcc                                                                               >  1:392722/1‑151 (MQ=255)                                                         aaTAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAAc                                                                            >  2:555344/1‑151 (MQ=255)                                                                            aaGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCAt                                                         >  2:335483/1‑151 (MQ=255)                                                                                cGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATacac                                                     <  1:52759/151‑1 (MQ=255)                                                                                   aaGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACAcc                                                    >  2:499742/1‑149 (MQ=255)                                                                                    aGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCaga                                                 <  2:199438/151‑1 (MQ=255)                                                                                            gggAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCGTACACCAGAGCACCGGc                                         <  1:549671/151‑1 (MQ=255)                                                                                              gAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGc                                         >  2:396250/1‑149 (MQ=255)                                                                                                 aTCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAgca                                     >  1:476498/1‑148 (MQ=255)                                                                                                            aacaaTGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAAcc                         >  1:386766/1‑151 (MQ=255)                                                                                                              caaTGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCAc                       <  1:193871/151‑1 (MQ=255)                                                                                                                            cgccgcTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACaaa          >  1:620312/1‑150 (MQ=255)                                                                                                                            cgccgcTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAAt         >  1:43014/1‑151 (MQ=255)                                                                                                                                 cTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGt    >  1:138587/1‑151 (MQ=255)                                                                                                                                    gCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGa  >  1:79199/1‑150 (MQ=255)                                                                                                                                          |                                                                                                                                                CATAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGA  >  NC_000913/1923853‑1924133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑