Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,954,771 A→G V746A (GTG→GCG)  torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,954,7710AG96.2% 82.6 / ‑3.3 26V746A (GTG→GCG) torZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (15/10);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

ATTGCCCGTATATTTTTCGATATACACCAACGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCACGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACG  >  NC_000913/1954623‑1954913
                                                                                                                                                    |                                                                                                                                              
aTTGCCCGTATATTTTTCGATATCCCCCAACGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGcgcg                                                                                                                                              <  2:264387/151‑1 (MQ=255)
 ttGCCCGTATATTTTTCGATATACACCAACGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGcgcg                                                                                                                                              <  2:287339/150‑1 (MQ=255)
        tataTTTTTCGATATACACCAACGCTGAGTTCCCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGTTATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCa                                                                                                                                       <  2:150400/150‑1 (MQ=255)
                    tataCACCAACGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGa                                                                                                                           <  2:171603/150‑1 (MQ=255)
                       acacCAACGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATccc                                                                                                                       >  1:582271/1‑151 (MQ=255)
                        cacCAACGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCg                                                                                                                      >  2:622384/1‑151 (MQ=255)
                            aaCGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCgtc                                                                                                                    >  2:16042/1‑149 (MQ=255)
                            aaCGCTGAGTCACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCgtc                                                                                                                    >  2:88153/1‑149 (MQ=255)
                                            cacaGTCATTGGCCAGCTGCGAGCGGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTg                                                                                                   <  2:349464/150‑1 (MQ=255)
                                                              gCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGt                                                                                  <  2:356261/149‑1 (MQ=255)
                                                                             ccgccgTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCAc                                                                 <  2:10503/151‑1 (MQ=255)
                                                                                ccgTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCAcc                                                                >  1:306803/1‑149 (MQ=255)
                                                                                     aaCACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGc                                                         <  1:551756/151‑1 (MQ=255)
                                                                                              gCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCa                                                 >  2:9504/1‑150 (MQ=255)
                                                                                               cACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAAt                                               >  1:118851/1‑151 (MQ=255)
                                                                                                   gCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATAcc                                            >  1:292926/1‑150 (MQ=255)
                                                                                                     cGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCaa                                          >  2:318673/1‑150 (MQ=255)
                                                                                                        ttttACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGc                                       <  2:582256/150‑1 (MQ=255)
                                                                                                        ttttACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGc                                       <  2:404776/150‑1 (MQ=255)
                                                                                                             cacaAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTgc                                   >  1:411114/1‑149 (MQ=255)
                                                                                                                 aGCCATTTTCCAGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCAt                              >  1:233845/1‑150 (MQ=255)
                                                                                                                  gCCATTTTCCAGATCTGGCCATGCACCTTCATGCACGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCt                            <  1:433955/151‑1 (MQ=255)
                                                                                                                            aGATCTGGCCATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGa                   >  2:51900/1‑150 (MQ=255)
                                                                                                                                    ccATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGAtt          >  2:244756/1‑151 (MQ=255)
                                                                                                                                     cATGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTg         <  1:622402/151‑1 (MQ=255)
                                                                                                                                       tGCACCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGt       >  1:368724/1‑151 (MQ=255)
                                                                                                                                          aCCTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCa    <  2:549736/151‑1 (MQ=255)
                                                                                                                                            cTTCATGCGCGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACg  >  2:291095/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
ATTGCCCGTATATTTTTCGATATACACCAACGCTGAGTTACCGGCACAGGCATTTGCCAGCTGCGAGCTGGGGATATCCGCCGTTAACACGTTCGCACTGCCGTTTTTACACAAGCCATTTTCCAGATCTGGCCATGCACCTTCATGCACGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACG  >  NC_000913/1954623‑1954913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑