Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,098,260	G→A	D14D (GAC→GAT)	wzzB ←	regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,098,260	0	G	A	100.0%	133.6 / NA	35	D14D (GAC→GAT)	wzzB	regulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/24);  total (11/24)

GTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAACA  >  NC_000913/2098114‑2098407                                                                                                                                                   |                                                                                                                                                    gTGATAATTGCTGTTGCGGTCCATTTCTCCTTCGCTACTGCAAAATACCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGTTTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCAt                                                                                                                                                 <  1:307386/151‑1 (MQ=255)    aTAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATg                                                                                                                                                <  1:365508/149‑1 (MQ=255)      aaTTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGttt                                                                                                                                            >  1:573932/1‑151 (MQ=255)                        tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACattatt                                                                                                                           <  1:593396/150‑1 (MQ=255)                        tttCTCCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCTTCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACatt                                                                                                                              <  2:337498/147‑1 (MQ=255)                        tttCTCCTCCGCTACGGCAAAATACCCAATAGCCAGGGCAATAGCCAAAATGACGAAAATGATGATTGTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACatt                                                                                                                              <  2:620702/147‑1 (MQ=255)                         ttCTCCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACattatta                                                                                                                          <  2:232811/150‑1 (MQ=255)                                 cGCTACTGCCAAATACCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTAc                                                                                                                  <  1:219576/150‑1 (MQ=255)                                         ccAAATATCAAATAGCCAGGACAATACCCAAAAGTACTAAAATAATGATTCTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcatc                                                                                                           <  2:549644/149‑1 (MQ=255)                                           aaaTATCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCAtt                                                                                                        <  2:574581/150‑1 (MQ=255)                                           aaaTATCCAATAGCCAGGGCAATAGCCAAAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTa                                                                                                       <  1:387089/151‑1 (MQ=255)                                              tatCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTAccc                                                                                                    >  2:603210/1‑151 (MQ=255)                                                 ccAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTa                                                                                                  >  1:120086/1‑150 (MQ=255)                                                     tAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTg                                                                                              >  2:456211/1‑150 (MQ=255)                                                                aTAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTcc                                                                                  >  1:360022/1‑151 (MQ=255)                                                                          tGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCAt                                                                        >  2:627632/1‑151 (MQ=255)                                                                              ggAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGtt                                                                    <  2:400198/151‑1 (MQ=255)                                                                                aaaTGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTa                                                                  <  1:514982/151‑1 (MQ=255)                                                                                   tgatgaTTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCg                                                               >  1:73232/1‑151 (MQ=255)                                                                                         ttGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAAt                                                          <  1:175296/150‑1 (MQ=255)                                                                                               tCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTc                                                      <  1:541999/148‑1 (MQ=255)                                                                                                 ttGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGa                                                  <  1:96195/150‑1 (MQ=255)                                                                                                    ccacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATg                                               <  1:133832/150‑1 (MQ=255)                                                                                                     cacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCt                                             >  1:280319/1‑151 (MQ=255)                                                                                                        gccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCtat                                           >  2:264544/1‑150 (MQ=255)                                                                                                           acaACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAgg                                        >  1:592104/1‑150 (MQ=255)                                                                                                           acaACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGa                                       <  1:163715/151‑1 (MQ=255)                                                                                                              aCTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATaa                                    <  1:580427/151‑1 (MQ=255)                                                                                                                 gCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGt                                  <  1:339737/150‑1 (MQ=255)                                                                                                                      aGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATaa                             >  2:404365/1‑150 (MQ=255)                                                                                                                             caatcaaatcaatcTGTTCCGGATCATGGTTTTGCTCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACAGAAATTTCCAAGTCCGCATAGTATAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAaataat                      <  2:901/150‑1 (MQ=255)                                                                                                                             caatcaaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATg                     <  1:603225/151‑1 (MQ=255)                                                                                                                                 caaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAtt                 <  2:123060/151‑1 (MQ=255)                                                                                                                                   aatcaatcTGTGCCGGATCATGGTTTTGCGCAGAAACATTATTAGTTTCTACTCTCAGCATTACCCGAACTGGGGGGAATTTGGAGGGGCGCATAGGGTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAttgt               <  2:357854/151‑1 (MQ=255)                                                                                                                                                cGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAACa  <  1:477600/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAACA  >  NC_000913/2098114‑2098407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑