Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,117,002	T→C	T42T (ACA→ACG)	wcaL ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,117,002	0	T	C	100.0%	108.9 / NA	31	T42T (ACA→ACG)	wcaL	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/24);  total (7/24)

GCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATG  >  NC_000913/2116854‑2117142                                                                                                                                                     |                                                                                                                                             gcgcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgt                                                                                                                                            <  2:504132/151‑1 (MQ=255)  cgcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgtg                                                                                                                                           >  2:83466/1‑151 (MQ=255)                    aCGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgt                                                                                                                                            >  2:335546/1‑132 (MQ=255)                    aCGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgt                                                                                                                                            <  1:335553/132‑1 (MQ=255)                         gAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGaa                                                                                                                     <  1:146106/150‑1 (MQ=255)                          aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAtc                                                                                                                   >  2:334608/1‑151 (MQ=255)                               cgcgCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTAc                                                                                                              <  2:523539/151‑1 (MQ=255)                                 cgcAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCt                                                                                                            <  2:584172/151‑1 (MQ=255)                                  gcAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTc                                                                                                           <  2:213722/151‑1 (MQ=255)                                       gTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATc                                                                                                      <  1:457447/151‑1 (MQ=255)                                          tggctggcTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATccc                                                                                                    >  1:232315/1‑150 (MQ=255)                                               ggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCatat                                                                                                <  1:613423/149‑1 (MQ=255)                                               ggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCatat                                                                                                <  1:83105/149‑1 (MQ=255)                                                   cGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATa                                                                                           <  1:604857/150‑1 (MQ=255)                                                       ggCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAAcg                                                                                       <  1:227517/150‑1 (MQ=255)                                                                tCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTg                                                                             >  2:424288/1‑151 (MQ=255)                                                                 cGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTgg                                                                            >  1:361427/1‑151 (MQ=255)                                                                 cGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTg                                                                             <  2:581443/150‑1 (MQ=255)                                                                        ttGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAg                                                                       <  2:232310/149‑1 (MQ=255)                                                                                    tCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTGTTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCt                                                          <  2:435266/150‑1 (MQ=255)                                                                                    tCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCt                                                          <  2:71912/150‑1 (MQ=255)                                                                                    tCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTg                                                         >  1:444213/1‑151 (MQ=255)                                                                                     cGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCt                                                          <  2:239116/149‑1 (MQ=255)                                                                                             aaCCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGc                                                 <  2:161045/150‑1 (MQ=255)                                                                                                cAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGa                                             <  2:462577/151‑1 (MQ=255)                                                                                                  gCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGaa                                            <  2:356296/150‑1 (MQ=255)                                                                                                  gCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAAt                                           <  1:120552/151‑1 (MQ=255)                                                                                                      gTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCa                                       <  1:28264/151‑1 (MQ=255)                                                                                                        tCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAg                                      <  2:274088/150‑1 (MQ=255)                                                                                                           ggCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGt                                     <  1:154009/148‑1 (MQ=255)                                                                                                                         aTTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCt                     <  2:338079/150‑1 (MQ=255)                                                                                                                             cGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCAt                 <  1:537876/150‑1 (MQ=255)                                                                                                                                    gCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCt          <  2:211941/150‑1 (MQ=255)                                                                                                                                      cgcgTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTccc       >  1:81956/1‑151 (MQ=255)                                                                                                                                           gggTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATg  >  1:433536/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                             GCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATG  >  NC_000913/2116854‑2117142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑