Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,228,525	G→A	pseudogene (85/1287 nt)	mdtQ ←	pseudogene; putative channel/filament proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,228,525	0	G	A	100.0%	137.3 / NA	35	pseudogene (85/1287 nt)	mdtQ	pseudogene; putative channel/filament proteins
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/18);  total (17/18)

AAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGGTGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAACGCCTG  >  NC_000913/2228382‑2228671                                                                                                                                                |                                                                                                                                                   aaaCGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTc                                                                                                                                             >  1:177323/1‑151 (MQ=255)        cccATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTg                                                                                                                                      <  1:561984/151‑1 (MQ=255)                  cTTCTGCCGACATTTTTTGCCGCCCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGttaat                                                                                                                            >  2:617445/1‑151 (MQ=255)                       gCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAgg                                                                                                                       <  2:564266/151‑1 (MQ=255)                                ttttGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGtt                                                                                                              >  2:414549/1‑151 (MQ=255)                                  ttGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTg                                                                                                             <  1:563431/150‑1 (MQ=255)                                    gCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGAtt                                                                                                          <  2:82864/151‑1 (MQ=255)                                    gCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGAt                                                                                                           <  2:422247/150‑1 (MQ=255)                                     ccGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGAtt                                                                                                          >  2:166201/1‑150 (MQ=255)                                            atatCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGAt                                                                                                  >  1:573759/1‑151 (MQ=255)                                              atCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATa                                                                                                 <  2:164672/150‑1 (MQ=255)                                               tCCGCCGAAAAGTCGATCTGCGGACCATCCTGGGTGGCAACTGCTTTCGCCTGGGCTTCACATAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTCGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACt                                                                                               >  2:490752/1‑151 (MQ=255)                                                  gccgAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCa                                                                                            <  2:171151/151‑1 (MQ=255)                                                           tCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCAtt                                                                                   >  2:137541/1‑151 (MQ=255)                                                                 tGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGt                                                                             >  2:602554/1‑151 (MQ=255)                                                                  gCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTgg                                                                                 <  2:301429/146‑1 (MQ=255)                                                                  gCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGc                                                                                <  1:452984/147‑1 (MQ=255)                                                                  gCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCt                                                                               <  2:239167/148‑1 (MQ=255)                                                                     gACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTgg                                                                         <  1:549752/151‑1 (MQ=255)                                                                        cATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGcc                                                                       >  1:32749/1‑150 (MQ=255)                                                                         aTCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCa                                                                      >  2:548098/1‑150 (MQ=255)                                                                              gAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCAtt                                                                >  2:494005/1‑151 (MQ=255)                                                                                      aCTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAgcgc                                                        <  2:22314/151‑1 (MQ=255)                                                                                             tCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGt                                                 >  1:199732/1‑151 (MQ=255)                                                                                              cGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTa                                                <  2:493106/151‑1 (MQ=255)                                                                                                                   aaTGCGCTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCCGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGc                             >  2:57404/1‑149 (MQ=255)                                                                                                                      gcgcTGTTCAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCgg                        <  1:137785/151‑1 (MQ=255)                                                                                                                              cAGCCACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGc                <  2:123600/151‑1 (MQ=255)                                                                                                                                  cACCTGCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGc            <  1:477551/151‑1 (MQ=255)                                                                                                                                       gCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAAc       <  2:462991/151‑1 (MQ=255)                                                                                                                                       gCATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAAc       >  1:598869/1‑151 (MQ=255)                                                                                                                                        cATATCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAACg      >  1:218773/1‑151 (MQ=255)                                                                                                                                         atatCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAACGc     >  2:542532/1‑151 (MQ=255)                                                                                                                                           atCCGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAACGCCt   >  1:173278/1‑151 (MQ=255)                                                                                                                                             ccGATGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAACGCCTg  <  2:490966/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   AAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCGGTGCGTTCTGTAGTGCATTGTTAATTAAGGAAGTGAGTTGATTATCGTGATACTCCAGCCACCATTGGCTGTCTGGCCAACCATTTTCAGCGCCGTGGGTAATGCGGTGTCAACTTGTGCAGCGGGCGTTTGCTGGCTTAACGCCTG  >  NC_000913/2228382‑2228671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑