Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,241,409 T→C Q87R (CAG→CGG)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,241,4090TC97.1% 117.7 / ‑3.6 34Q87R (CAG→CGG) galSgalactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (18/15);  total (19/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

ATCCATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACG  >  NC_000913/2241261‑2241552
                                                                                                                                                    |                                                                                                                                               
aTCCATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGAtgccgc                                                                                                                                               >  1:412303/1‑151 (MQ=255)
      aaaTTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGc                                                                                                                                          <  2:359642/150‑1 (MQ=255)
      aaaTTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCg                                                                                                                                         <  1:382510/151‑1 (MQ=255)
        aTTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGAc                                                                                                                                       >  1:199475/1‑151 (MQ=255)
             gcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGAt                                                                                                                                  <  2:414546/151‑1 (MQ=255)
                 gTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCAc                                                                                                                              >  2:509738/1‑151 (MQ=255)
                  ttCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATcc                                                                                                                                <  1:490151/148‑1 (MQ=255)
                  ttCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATcc                                                                                                                                >  2:490141/1‑148 (MQ=255)
                    cATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGc                                                                                                                           >  1:154409/1‑151 (MQ=255)
                       cGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCttt                                                                                                                        >  2:540725/1‑151 (MQ=255)
                         tCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCtttt                                                                                                                       >  2:529780/1‑150 (MQ=255)
                                     tttGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCg                                                                                                           <  1:108229/150‑1 (MQ=255)
                                      ttGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGa                                                                                                          >  1:492818/1‑150 (MQ=255)
                                         aGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaaa                                                                                                       >  2:70609/1‑150 (MQ=255)
                                            gAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACg                                                                                                    <  1:142808/150‑1 (MQ=255)
                                                aaTCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCa                                                                                               <  2:323805/151‑1 (MQ=255)
                                                    aaCGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGaa                                                                                            >  1:510589/1‑150 (MQ=255)
                                                      cGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACg                                                                                         <  2:625020/151‑1 (MQ=255)
                                                                     gCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACg                                                                          >  1:435905/1‑151 (MQ=255)
                                                                      cGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGc                                                                         >  2:184722/1‑151 (MQ=255)
                                                                      cGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGc                                                                         >  1:272101/1‑151 (MQ=255)
                                                                       gAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGcc                                                                        >  2:561815/1‑151 (MQ=255)
                                                                       gAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGcc                                                                        <  1:373858/151‑1 (MQ=255)
                                                                           tAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATg                                                                    >  1:218323/1‑151 (MQ=255)
                                                                                       ggCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAAcc                                                        <  2:6525/151‑1 (MQ=255)
                                                                                            gACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGt                                                   <  2:435896/151‑1 (MQ=255)
                                                                                                         cGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTgg                                      >  1:576790/1‑151 (MQ=255)
                                                                                                              cATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGCCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCAtt                                 <  2:44973/151‑1 (MQ=255)
                                                                                                                tGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTgg                               >  2:355315/1‑151 (MQ=255)
                                                                                                                  aTAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCg                             <  2:191290/151‑1 (MQ=255)
                                                                                                                        aTTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGc                       <  2:218318/151‑1 (MQ=255)
                                                                                                                            ccGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGAt                   >  2:546847/1‑151 (MQ=255)
                                                                                                                                     aCGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGAGAATCCAGc           <  2:433705/150‑1 (MQ=255)
                                                                                                                                             cTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACg  >  1:50069/1‑151 (MQ=255)
                                                                                                                                             cTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACg  >  1:527834/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ATCCATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACG  >  NC_000913/2241261‑2241552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑