Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,254,920	G→A	G89G (GGC→GGT)	rihB ←	ribonucleoside hydrolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,254,920	0	G	A	100.0%	195.4 / NA	52	G89G (GGC→GGT)	rihB	ribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/27);  total (25/27)

TTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTC  >  NC_000913/2254776‑2255064                                                                                                                                                 |                                                                                                                                                 ttGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATcc                                                                                                                                            >  1:290424/1‑151 (MQ=255)   gCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATcc                                                                                                                                            >  2:214184/1‑149 (MQ=255)       aCGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTcc                                                                                                                                       <  2:435720/150‑1 (MQ=255)         gCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCgg                                                                                                                                     >  1:290399/1‑150 (MQ=255)         gCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGt                                                                                                                                    >  1:77952/1‑151 (MQ=255)              gCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCa                                                                                                                                <  2:74297/150‑1 (MQ=255)               ccACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCAcc                                                                                                                              >  2:444107/1‑151 (MQ=255)                        tatTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAAtatt                                                                                                                     >  2:43554/1‑151 (MQ=255)                         atTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAAtatta                                                                                                                    <  1:533996/151‑1 (MQ=255)                           ttGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAAtattat                                                                                                                   <  2:183187/150‑1 (MQ=255)                             gAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCg                                                                                                                 <  1:521450/150‑1 (MQ=255)                               aaGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCa                                                                                                              <  1:214235/151‑1 (MQ=255)                                 gCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAAc                                                                                                            <  2:632048/151‑1 (MQ=255)                                      ccAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGAttt                                                                                                       >  2:291243/1‑151 (MQ=255)                                        aaCCGGCACCAGAGTGATATCGCCATCGCTTCCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATttgt                                                                                                     <  2:29644/151‑1 (MQ=255)                                           cGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGa                                                                                                  <  1:550579/151‑1 (MQ=255)                                                 cAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATa                                                                                            <  1:313504/151‑1 (MQ=255)                                                    agTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATg                                                                                         <  1:70719/151‑1 (MQ=255)                                                           tCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGc                                                                                   >  1:143847/1‑150 (MQ=255)                                                                  cGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATccc                                                                           >  2:359361/1‑151 (MQ=255)                                                                    cTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATcccc                                                                          >  2:71389/1‑150 (MQ=255)                                                                      tGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGc                                                                        >  1:296471/1‑150 (MQ=255)                                                                         cATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATaa                                                                    <  2:302447/151‑1 (MQ=255)                                                                            cAGGGTATCAATGATATATTTCCCCGCATGAGTGCTTTCTGCTTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAAc                                                                                                            <  1:536043/108‑1 (MQ=255)                                                                            cAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAAc                                                                                                            >  2:536032/1‑108 (MQ=255)                                                                                    cAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACAtt                                                         >  2:152465/1‑151 (MQ=255)                                                                                    cAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACAt                                                          <  2:75757/150‑1 (MQ=255)                                                                                     aaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTa                                                        <  1:352236/151‑1 (MQ=255)                                                                                        gATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAAtc                                                     >  1:540845/1‑151 (MQ=255)                                                                                        gATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAAtc                                                     <  2:432597/151‑1 (MQ=255)                                                                                         atatatTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAAtct                                                    >  2:51578/1‑151 (MQ=255)                                                                                                 cACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGttt                                             <  1:391693/150‑1 (MQ=255)                                                                                                 cACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTc                                            >  2:606515/1‑151 (MQ=255)                                                                                                     gCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGc                                        >  2:265793/1‑151 (MQ=255)                                                                                                     gCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGc                                        >  2:536345/1‑151 (MQ=255)                                                                                                     gCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGc                                        <  1:494997/151‑1 (MQ=255)                                                                                                      cATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGc                                        >  1:273640/1‑150 (MQ=255)                                                                                                       aTGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCaa                                      <  1:230182/151‑1 (MQ=255)                                                                                                             gCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTc                                >  2:20200/1‑151 (MQ=255)                                                                                                                 tCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAgg                             <  1:579218/150‑1 (MQ=255)                                                                                                                   tGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGcc                           >  2:603535/1‑150 (MQ=255)                                                                                                                   tGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGGGAATATTATCGGCCACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGcc                           >  2:58476/1‑150 (MQ=255)                                                                                                                    gCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGc                            <  1:455997/148‑1 (MQ=255)                                                                                                                          cGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCattaatta                   <  1:413575/151‑1 (MQ=255)                                                                                                                             gTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATg                <  2:391485/151‑1 (MQ=255)                                                                                                                               cAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGtt              <  1:198224/151‑1 (MQ=255)                                                                                                                               cAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGtt              <  1:142138/151‑1 (MQ=255)                                                                                                                                    gCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATc         <  2:323100/151‑1 (MQ=255)                                                                                                                                       cGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCa        >  1:597575/1‑149 (MQ=255)                                                                                                                                          aTACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGt   <  2:523954/151‑1 (MQ=255)                                                                                                                                           tACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTc  >  2:528495/1‑151 (MQ=255)                                                                                                                                           tACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTc  >  2:26211/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTC  >  NC_000913/2254776‑2255064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑