Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,399,151 Δ1 bp coding (1740/1848 nt) mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,399,1510G.100.0% 747.2 / NA 150coding (1740/1848 nt)mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (65/85);  total (65/85)

Only 100 of 150 total aligned reads displayed.

CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTT  >  NC_000913/4399005‑4399295
                                                                                                                                                  |                                                                                                                                                
caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGc                                                                                                                                             <  4:1569869/151‑1 (MQ=255)
caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGc                                                                                                                                             >  1:1768385/1‑151 (MQ=255)
 aacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCg                                                                                                                                            >  4:114555/1‑151 (MQ=255)
       aTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAATGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTg                                                                                                                                      <  3:345310/151‑1 (MQ=255)
         ttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTgg                                                                                                                                     >  4:88397/1‑150 (MQ=255)
         ttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTgg                                                                                                                                     >  1:504489/1‑150 (MQ=255)
           aCAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAAc                                                                                                                                  <  1:1016830/151‑1 (MQ=255)
             aaaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACgg                                                                                                                                >  1:1210509/1‑151 (MQ=255)
             aaaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACgg                                                                                                                                <  4:1181131/151‑1 (MQ=255)
             aaaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACgg                                                                                                                                <  4:153390/151‑1 (MQ=255)
             aaaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACgg                                                                                                                                <  1:255847/151‑1 (MQ=255)
                   tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGtt                                                                                                                              <  3:161006/147‑1 (MQ=255)
                   tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGtt                                                                                                                              <  4:1777448/147‑1 (MQ=255)
                   tGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTAt                                                                                                                            <  1:624934/149‑1 (MQ=255)
                    gATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGt                                                                                                                          <  1:1821889/150‑1 (MQ=255)
                    gATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTc                                                                                                                         >  1:899883/1‑151 (MQ=255)
                     aTTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTcc                                                                                                                        <  4:1043417/151‑1 (MQ=255)
                       tCCTGAACTGATAGGCTAGCTGGCGGAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGc                                                                                                                      >  3:415860/1‑151 (MQ=255)
                           gAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCaa                                                                                                                    <  4:858192/149‑1 (MQ=255)
                           gAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACt                                                                                                                  <  1:1186373/151‑1 (MQ=255)
                            aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                                                                                                 <  3:394257/151‑1 (MQ=255)
                             aCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTtg                                                                                                                <  2:157613/151‑1 (MQ=255)
                                gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTtgtg                                                                                                              <  1:1236105/150‑1 (MQ=255)
                                gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTtgt                                                                                                               <  1:352467/149‑1 (MQ=255)
                                gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGa                                                                                                             <  1:1380061/151‑1 (MQ=255)
                                 aTAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaa                                                                                                            <  3:212284/151‑1 (MQ=255)
                                 aTAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGa                                                                                                             >  2:132762/1‑150 (MQ=255)
                                   aGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaaa                                                                                                           <  4:311003/150‑1 (MQ=255)
                                   aGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaaa                                                                                                           <  4:1060978/150‑1 (MQ=255)
                                   aGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaaa                                                                                                           <  1:676076/150‑1 (MQ=255)
                                        aCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccg                                                                                                     <  1:463461/151‑1 (MQ=255)
                                        aCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgcc                                                                                                      >  1:895606/1‑150 (MQ=255)
                                        aCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgc                                                                                                       <  4:384413/149‑1 (MQ=255)
                                         ccTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgcc                                                                                                      <  1:170742/149‑1 (MQ=255)
                                            ggCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACgccgcc                                                                                                   <  1:1028007/149‑1 (MQ=255)
                                            ggCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGccgccg                                                                                                  <  1:437404/150‑1 (MQ=255)
                                            ggCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCgg                                                                                                 <  3:384684/151‑1 (MQ=255)
                                              cGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggt                                                                                               <  2:234225/151‑1 (MQ=255)
                                              cGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggt                                                                                               <  4:207989/151‑1 (MQ=255)
                                                aaGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtgg                                                                                             >  1:1357886/1‑151 (MQ=255)
                                                aaGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtgg                                                                                             >  4:1229444/1‑151 (MQ=255)
                                                 aGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                                                                                            <  4:475414/151‑1 (MQ=255)
                                                 aGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGACGggtggg                                                                                            <  3:457517/151‑2 (MQ=255)
                                                  gCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTc                                                                                           >  1:763848/1‑151 (MQ=255)
                                                    aGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTg                                                                                         >  2:61278/1‑151 (MQ=255)
                                                    aGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTg                                                                                         >  4:1576591/1‑151 (MQ=255)
                                                     gTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                                                                                        <  2:294490/151‑1 (MQ=255)
                                                     gTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                                                                                        <  3:51003/151‑1 (MQ=255)
                                                     gTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGt                                                                                        >  4:1612021/1‑151 (MQ=255)
                                                       ccGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGtt                                                                                       <  4:514204/150‑1 (MQ=255)
                                                         gTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTAc                                                                                     >  4:1832704/1‑150 (MQ=255)
                                                           aTTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAAt                                                                                  >  3:261421/1‑151 (MQ=255)
                                                                aaCCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGtt                                                                             <  4:1541279/151‑1 (MQ=255)
                                                                  ccTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTg                                                                            >  4:1353326/1‑150 (MQ=255)
                                                                    tGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAtt                                                                         >  2:449831/1‑151 (MQ=255)
                                                                    tGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAtt                                                                         >  1:1347573/1‑151 (MQ=255)
                                                                     ggCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt                                                                        <  1:1151052/151‑1 (MQ=255)
                                                                      gCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTa                                                                       <  1:527643/151‑1 (MQ=255)
                                                                         atatTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACAt                                                                    >  4:454194/1‑151 (MQ=255)
                                                                           atTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATcc                                                                  <  4:937237/151‑1 (MQ=255)
                                                                           atTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATc                                                                   <  3:419370/150‑1 (MQ=255)
                                                                             tGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCg                                                                 <  4:855263/150‑1 (MQ=255)
                                                                              gcgcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGc                                                               <  4:75969/151‑1 (MQ=255)
                                                                               cgcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCg                                                              <  4:1099279/151‑1 (MQ=255)
                                                                                 cAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGa                                                             >  4:1109358/1‑150 (MQ=255)
                                                                                  aGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGAt                                                            <  1:1585059/150‑1 (MQ=255)
                                                                                  aGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATa                                                           <  1:1493825/151‑1 (MQ=255)
                                                                                  aGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATa                                                           <  3:225331/151‑1 (MQ=255)
                                                                                           cACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGGGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTg                                                  >  1:1709653/1‑151 (MQ=255)
                                                                                            aCGACATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTCCATCCGGCGATAAAAGCCCTGa                                                 <  1:25023/151‑1 (MQ=255)
                                                                                                aaTCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGaa                                                >  4:922699/1‑148 (MQ=255)
                                                                                                aaTCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGa                                             <  1:977394/151‑1 (MQ=255)
                                                                                                     gatgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGa                                          <  1:378271/149‑1 (MQ=255)
                                                                                                     gatgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGa                                          <  4:67492/149‑1 (MQ=255)
                                                                                                      atgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTg                                       <  2:426821/151‑1 (MQ=255)
                                                                                                      atgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTg                                       <  2:165666/151‑1 (MQ=255)
                                                                                                      atgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTg                                       <  4:450521/151‑1 (MQ=255)
                                                                                                        gaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGat                                     >  1:1158038/1‑151 (MQ=255)
                                                                                                        gaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGa                                      <  4:764177/150‑1 (MQ=255)
                                                                                                          gCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGata                                    <  1:449989/150‑1 (MQ=255)
                                                                                                            gAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATc                                  >  1:1772415/1‑150 (MQ=255)
                                                                                                            gAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATc                                  <  1:114522/150‑1 (MQ=255)
                                                                                                             aaCATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGatat                                   >  1:533902/1‑148 (MQ=255)
                                                                                                              aCATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGt                               >  4:89076/1‑151 (MQ=255)
                                                                                                              aCATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGt                               >  1:674127/1‑151 (MQ=255)
                                                                                                               cATGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTa                              <  1:1001043/151‑1 (MQ=255)
                                                                                                                aTGCGCAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTATGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTa                              >  2:470175/1‑150 (MQ=255)
                                                                                                                  gcgcAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAgg                           >  4:1889683/1‑151 (MQ=255)
                                                                                                                  gcgcAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAgg                           <  1:724508/151‑1 (MQ=255)
                                                                                                                  gcgcAGTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAgg                           <  3:33369/151‑1 (MQ=255)
                                                                                                                       gTGGTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAgc                      >  3:181179/1‑151 (MQ=255)
                                                                                                                         ggTCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAgcct                    >  1:1389579/1‑151 (MQ=255)
                                                                                                                           tCAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGGTATCAGTAAGGCGAgcctgc                  >  4:1478572/1‑151 (MQ=255)
                                                                                                                            cAATGGCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAgcctgcc                 >  1:1843422/1‑151 (MQ=255)
                                                                                                                                ggCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAg             <  4:649011/151‑1 (MQ=255)
                                                                                                                                ggCACAGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAg             <  2:51440/151‑1 (MQ=255)
                                                                                                                                     aGGCCATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGAt        <  4:362874/151‑1 (MQ=255)
                                                                                                                                         cATAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGAttttt    >  4:1758610/1‑151 (MQ=255)
                                                                                                                                          aTAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGAtttttt   >  1:434141/1‑151 (MQ=255)
                                                                                                                                          aTAACCCTCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGAtttttt   >  1:1006921/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTT  >  NC_000913/4399005‑4399295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: