Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,635 (C)5→6 coding (415/822 nt) yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6301.C99.4% 582.5 / NA 163G140G (GGC→GGG) yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (81/81);  minor base A (0/1);  total (81/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

GAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑CCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTTG  >  NC_000913/2449481‑2449774
                                                                                                                                                      |                                                                                                                                                
gAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                                                                                                   <  3:470373/150‑1 (MQ=255)
   aGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccc                                                                                                                                              >  1:1494405/1‑151 (MQ=255)
   aGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccc                                                                                                                                              <  4:1539579/151‑1 (MQ=255)
   aGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccc                                                                                                                                              >  1:348123/1‑151 (MQ=255)
   aGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccc                                                                                                                                              >  1:76036/1‑151 (MQ=255)
      gTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccccct                                                                                                                                           <  4:530532/151‑3 (MQ=255)
      gTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccccct                                                                                                                                           <  1:550467/151‑3 (MQ=255)
        tttCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccccctg                                                                                                                                          <  4:1650736/150‑4 (MQ=255)
        tttCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccccctg                                                                                                                                          <  4:1283374/150‑4 (MQ=255)
             cAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGagag                                                                                                                                      <  1:1076587/150‑1 (MQ=255)
               aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCt                                                                                                                                   <  2:192482/151‑1 (MQ=255)
                ccACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCtt                                                                                                                                  <  1:1821102/151‑1 (MQ=255)
                ccACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCtt                                                                                                                                  <  4:1117240/151‑1 (MQ=255)
                 cacaCCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTc                                                                                                                                 <  1:1542221/151‑1 (MQ=255)
                   tacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTc                                                                                                                                 >  1:1467586/2‑149 (MQ=255)
                   cacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTc                                                                                                                                 >  1:138269/1‑149 (MQ=255)
                    acCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTc                                                                                                                                 >  4:1288618/1‑148 (MQ=255)
                     ccGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCagag                                                                                                                             <  1:1720319/151‑1 (MQ=255)
                      cGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGt                                                                                                                            >  1:1790159/1‑151 (MQ=255)
                      cGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGt                                                                                                                            <  1:1069222/151‑1 (MQ=255)
                       gCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtc                                                                                                                           >  1:219518/1‑151 (MQ=255)
                        cccGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtca                                                                                                                          <  3:365564/151‑1 (MQ=255)
                          cGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcatt                                                                                                                        >  1:1286461/1‑151 (MQ=255)
                             cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                                     <  4:890488/151‑1 (MQ=255)
                                cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                                  >  4:1445299/1‑151 (MQ=255)
                                  gCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                                                                                                 <  2:457241/150‑1 (MQ=255)
                                  gCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                                  <  2:313572/149‑1 (MQ=255)
                                    aGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                                                                              <  4:1658261/151‑1 (MQ=255)
                                    aGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑cccccct                                                                                                                                           >  4:1448319/1‑119 (MQ=255)
                                       gTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAACCGTCATAAGGTTGTACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGGGAGGCTTCAGAGGCATTCATAATaaccaac                                                                                                           >  3:310804/1‑151 (MQ=255)
                                       gTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaac                                                                                                           >  1:1801672/1‑151 (MQ=255)
                                       gTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaa                                                                                                            >  4:1678860/1‑150 (MQ=255)
                                        tGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCAT‑AGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCCTTCATAATaaccaacc                                                                                                          >  1:1341688/1‑150 (MQ=255)
                                         gAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAaccaacca                                                                                                         <  4:1031320/151‑1 (MQ=255)
                                          aGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAt                                                                                                        <  1:1110772/151‑1 (MQ=255)
                                              gTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAATGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                                                                                    >  3:342691/1‑151 (MQ=255)
                                                tttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACa                                                                                                   <  1:1097739/150‑1 (MQ=255)
                                                   gACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGa                                                                                                <  1:1449495/150‑1 (MQ=255)
                                                   gACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGa                                                                                                >  1:687111/1‑150 (MQ=255)
                                                     cTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaaa                                                                                              >  1:1620/1‑150 (MQ=255)
                                                      ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaaa                                                                                              >  2:62211/1‑149 (MQ=255)
                                                       tACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                                                                                            <  2:475278/150‑1 (MQ=255)
                                                        aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGt                                                                                           >  4:1925484/1‑150 (MQ=255)
                                                        aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                                                                                          <  4:1786060/151‑1 (MQ=255)
                                                           gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTaa                                                                                         <  4:1396194/149‑1 (MQ=255)
                                                           gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAg                                                                                        <  3:484623/150‑1 (MQ=255)
                                                           gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                                                                       <  4:1574763/151‑1 (MQ=255)
                                                             cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                                                                                     >  3:448966/1‑151 (MQ=255)
                                                              aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                                    >  1:1833520/1‑151 (MQ=255)
                                                              aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                                    >  4:1090883/1‑151 (MQ=255)
                                                                aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCaa                                                                                  <  4:1752250/151‑1 (MQ=255)
                                                                 aTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAt                                                                                 <  2:209729/151‑1 (MQ=255)
                                                                   tctcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtct                                                                               >  4:476961/1‑151 (MQ=255)
                                                                   tctcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc                                                                                >  1:1751333/1‑150 (MQ=255)
                                                                         tCCATGAAACGTCATAAGGTTGAACAGAAACGGCTGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                                                                           >  4:1867305/1‑149 (MQ=255)
                                                                            aTGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAgaga                                                                      <  3:56814/151‑1 (MQ=255)
                                                                               aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACaa                                                                   <  1:1759952/151‑1 (MQ=255)
                                                                               aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACa                                                                    >  4:1799996/1‑150 (MQ=255)
                                                                                 aCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAAt                                                                  >  1:887429/1‑150 (MQ=255)
                                                                                 aCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAAt                                                                  >  1:1240065/1‑150 (MQ=255)
                                                                                     cATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc                                                              <  4:393/150‑1 (MQ=255)
                                                                                     cATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCt                                                             >  2:124629/1‑151 (MQ=255)
                                                                                       tAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTc                                                            >  2:113965/1‑150 (MQ=255)
                                                                                       tAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTc                                                            >  4:1799248/1‑150 (MQ=255)
                                                                                        aaGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCa                                                           >  1:1352784/1‑150 (MQ=255)
                                                                                        aaGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCa                                                           <  4:1128841/150‑1 (MQ=255)
                                                                                           gTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATg                                                       <  4:1704607/151‑1 (MQ=255)
                                                                                              gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGta                                                     <  1:251940/150‑1 (MQ=255)
                                                                                               aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtata                                                   <  2:388601/151‑1 (MQ=255)
                                                                                               aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                                    >  3:101744/1‑150 (MQ=255)
                                                                                                aCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtata                                                   >  4:1901591/1‑150 (MQ=255)
                                                                                                  aGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTataat                                                 >  1:1217706/1‑150 (MQ=255)
                                                                                                  aGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTataat                                                 >  1:984563/1‑150 (MQ=255)
                                                                                                   gAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAc                                               <  1:1406952/151‑1 (MQ=255)
                                                                                                    aaaCGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAcc                                              <  2:283187/151‑1 (MQ=255)
                                                                                                      aCGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCg                                            >  3:27670/1‑151 (MQ=255)
                                                                                                      aCGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCg                                            <  1:792093/151‑1 (MQ=255)
                                                                                                       cGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGc                                           <  4:1385932/151‑1 (MQ=255)
                                                                                                        ggATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGATAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGc                                           >  1:1278703/1‑150 (MQ=255)
                                                                                                        ggATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGc                                           >  4:60725/1‑150 (MQ=255)
                                                                                                             gACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTcc                                     <  1:1088966/151‑1 (MQ=255)
                                                                                                                 aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTCCCAccc                                 <  3:444782/151‑1 (MQ=255)
                                                                                                                     gtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGAc                             <  3:520964/151‑1 (MQ=255)
                                                                                                                            gACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATc                      <  3:324892/151‑1 (MQ=255)
                                                                                                                            gACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATc                      <  4:322546/151‑1 (MQ=255)
                                                                                                                             aCTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATcc                     >  1:1379471/1‑151 (MQ=255)
                                                                                                                              cTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATcc                     >  4:189395/1‑150 (MQ=255)
                                                                                                                              cTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATcc                     >  1:1646552/1‑150 (MQ=255)
                                                                                                                              cTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATcc                     >  1:1052491/1‑150 (MQ=255)
                                                                                                                               tATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATcc                     >  1:496818/1‑149 (MQ=255)
                                                                                                                                aTCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAgg                  <  2:258179/151‑1 (MQ=255)
                                                                                                                                 tCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAgg                  >  2:517526/1‑150 (MQ=255)
                                                                                                                                  cAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGAc                <  1:1447498/151‑1 (MQ=255)
                                                                                                                                         cTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAg                               >  3:101944/1‑129 (MQ=255)
                                                                                                                                           gATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATATCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCtt       >  4:771884/1‑151 (MQ=255)
                                                                                                                                             tACGTCACGACCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGACAAGTAAGATGCAATCTCTGCAGAGACAGTAACCTCAAATGTACAATACCCGCAGTTCCACCCGGACGGTAATCCAGGACAACTTGCttt      <  1:379404/150‑1 (MQ=255)
                                                                                                                                              aCGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTttgt    <  1:487919/151‑1 (MQ=255)
                                                                                                                                               cGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTttgtt   <  4:1299065/151‑1 (MQ=255)
                                                                                                                                                gTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTttgttg  >  2:249840/1‑151 (MQ=255)
                                                                                                                                                 tCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTttgttg  >  3:182414/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑CCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTTG  >  NC_000913/2449481‑2449774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑