Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT41.7% 161.4 / 92.9 145intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (12/72);  new base T (60/0);  total (73/72)
Fisher's exact test for biased strand distribution p-value = 1.53e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206285‑3206584
                                                                                                                                                       |                                                                                                                                                         
    gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGa                                                                                                                                                       <  4:1920580/151‑1 (MQ=255)
      aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑Ca                                                                                                                                                    <  4:1598306/151‑1 (MQ=255)
       tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTCAGaaa                                                                                                                                                     >  1:426408/1‑148 (MQ=255)
        ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑Caa                                                                                                                                                   <  4:1575053/150‑1 (MQ=255)
         tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑Caaa                                                                                                                                                  <  1:1645596/150‑1 (MQ=255)
          cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑aaaa                                                                                                                                                  >  1:1407481/1‑149 (MQ=255)
               aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACaaaa                                                                                                                                          <  1:820088/151‑1 (MQ=255)
                aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTTATA‑Caaa‑aaaaaaa                                                                                                                                          >  1:1359504/1‑150 (MQ=255)
                 cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAAc                                                                                                                                        <  4:894769/151‑1 (MQ=255)
                  aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑TAaaaaaa                                                                                                                                         >  4:645846/1‑149 (MQ=255)
                  aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑AAAA‑TACaaaaa                                                                                                                                         >  4:1363958/1‑149 (MQ=255)
                       cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGa                                                                                                                                    <  4:1177624/149‑1 (MQ=255)
                        aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGtt                                                                                                                                 <  1:1617814/151‑1 (MQ=255)
                        aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGtt                                                                                                                                 <  1:1363401/151‑1 (MQ=255)
                        aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGtt                                                                                                                                 <  3:322151/151‑1 (MQ=255)
                        aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCCCA‑CAAA‑TAAAAAAAAAGGAGt                                                                                                                                  >  1:1254753/1‑150 (MQ=255)
                            ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCa                                                                                                                             <  4:351982/151‑1 (MQ=255)
                                  ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCatgat                                                                                                                         <  4:1780650/149‑1 (MQ=255)
                                   tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGAATTTTTCATA‑CAAA‑AAAAAAAAATGGAggt                                                                                                                                 >  1:1501492/1‑138 (MQ=255)
                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAg                                                                                                                      <  1:582857/150‑1 (MQ=255)
                                     gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑AAAAAAAAATGGAGCTgttcttaatag                                                                                                                      >  1:768518/1‑139 (MQ=255)
                                     gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAA‑CAAA‑AACAAAAACTGGAGTTGCCCTGATGAGc                                                                                                                     >  2:103534/1‑150 (MQ=255)
                                      cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑AGAATTTTTTCCGA‑CAAA‑TACAAAAAATGGGGGTGCCATGATGAGCg                                                                                                                    >  2:269426/1‑150 (MQ=255)
                                       gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCAAA‑CAAA‑AAAAAAAAATGGAGTTGCCATGATGAGCg                                                                                                                    >  4:1056514/1‑149 (MQ=255)
                                        cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                                 <  1:1825719/151‑1 (MQ=255)
                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTATA‑CAAA‑AACAAAAAATTGAGTTGTCATGATGAGCGaa                                                                                                                  >  1:1239871/1‑149 (MQ=255)
                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGA‑CAAA‑AACAAAAAATGGAGGTGGCATGATGAGCGaaa                                                                                                                 >  4:1816831/1‑150 (MQ=255)
                                          atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGt                                                                                                               <  3:326394/151‑1 (MQ=255)
                                           tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGACCAAA‑CAAAAAAATGGGAGTGGcctgattggagaaa                                                                                                                  >  3:459340/1‑136 (MQ=255)
                                            attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGA‑CAAA‑AACAAAAAATGGAGTTGCCATGATGAGCGaaa                                                                                                                 >  4:1735451/1‑147 (MQ=255)
                                            attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGA‑CAAA‑AAAAAAAAATGGAGTTGCCCTGATGAGCGaaa                                                                                                                 >  4:1620546/1‑147 (MQ=255)
                                            attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                              <  2:155907/150‑1 (MQ=255)
                                              tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAA‑AAAA‑AACAAAAAATGGAGGTTGCATGATGAGCGAAAGtaa                                                                                                             >  1:620807/1‑149 (MQ=255)
                                              tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                            <  2:136472/150‑1 (MQ=255)
                                               cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑AACAAAAAATGGAGTTGGCATGATGAGCGAAAGaaat‑a                                                                                                          >  4:1071798/1‑150 (MQ=255)
                                               cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat‑aa                                                                                                         <  4:435755/151‑1 (MQ=255)
                                                  tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTTAAA‑AAAA‑AACAAAAAAATGAGTTGGCATTATGGGGGGAAGgaat‑aa                                                                                                         >  1:456763/1‑148 (MQ=255)
                                                  tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAA‑AAAA‑AAAAAAAAAAGGAGTTGCCATGATGAGGGAAAGaaaa‑aa                                                                                                         >  2:426433/1‑148 (MQ=255)
                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCa                                                                                                      <  1:373539/148‑1 (MQ=255)
                                                          tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAA‑CAAA‑AAAAAAAAATGGAGTTGCCATGATGAGGGGAAGgaaa‑aaaaaaaaag                                                                                                 >  2:341178/1‑140 (MQ=255)
                                                           cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAg                                                                                             <  1:1229129/150‑1 (MQ=255)
                                                           cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGt                                                                                            <  2:478390/151‑1 (MQ=255)
                                                            gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTg                                                                                           <  4:1713206/151‑1 (MQ=255)
                                                             cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGa                                                                                          <  2:230281/151‑1 (MQ=255)
                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑AAAAAAAACCCGAGGTGGCCTGATGAGCGAAAGGAAT‑AAACaaaaagacatg                                                                                            >  4:1799213/1‑138 (MQ=255)
                                                                     cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTg                                                                                  <  4:627452/151‑1 (MQ=255)
                                                                      aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TTGATTTTTTCAGA‑AAAA‑AAAAAAAAAAGTAGATGCCATGGTGAACGAAAGAAAT‑AAAAAAAA‑AGCAGGGAaaaaaat                                                                                   >  4:1766899/1‑146 (MQ=255)
                                                                      aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGa                                                                                 <  2:334562/151‑1 (MQ=255)
                                                                       cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAATTTTT‑CTGA‑CAAATTAAAAAAACATATTTTGCTATGTTGGGGGAATATAAA‑AAG‑ACCA‑GGCAGTGAATaa                                                                                      >  1:1275622/1‑145 (MQ=255)
                                                                             gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TGCAAAAACTGGAGTTGCGATGATGAGCGAAAGTAAT‑AAGCAACA‑TGCAGTGAATAAGTTGACAGAGAt                                                                          <  3:79452/151‑1 (MQ=255)
                                                                             gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAgaga                                                                           <  4:82709/150‑1 (MQ=255)
                                                                             gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGAt                                                                          <  3:184128/151‑1 (MQ=255)
                                                                                aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGt                                                                       <  4:578877/151‑1 (MQ=255)
                                                                                aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTCCCGA‑CAAA‑AACAAAAACTAAAGTTGACACGATGAGCGAAAAGAATAAAACAAAA‑GACAG‑GAAAAAGATGACag                                                                              >  3:247963/1‑144 (MQ=255)
                                                                                aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑AGAATTTTTTCAAA‑CAAA‑AACAAAAAATGGAGTTGGCATTATGAGCGAAAAGAAA‑AAGCAACA‑GGGAGGGAATAAATTGACAGAGAtt                                                                         >  2:283210/1‑149 (MQ=255)
                                                                                  ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTc                                                                      <  4:1406217/150‑1 (MQ=255)
                                                                                   tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCAAA‑CAAA‑AAAAAAAAATGGAGTTGCCATGATGGGGGGAAGGAAT‑AAAAAAAA‑AGCAGGGAAAAAGGTGACAGAGAATGttg                                                                     >  1:1081211/1‑148 (MQ=255)
                                                                                    aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGTGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                   <  1:1446557/151‑1 (MQ=255)
                                                                                     cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑AAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAAT‑AAACAACA‑AGCAGTGAATAAATTGACAGAGATTGt                                                                       >  2:520221/1‑146 (MQ=255)
                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑TAAAAAAAATGGGGTTGGCATGATGAGCGAAAGTAAT‑AAACAACA‑AGCAGGGAATAAGTTGACAGAGATTGTCg                                                                     >  4:1334482/1‑147 (MQ=255)
                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTaa                                                                 <  4:145117/151‑1 (MQ=255)
                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTTAGA‑CAAA‑TACAAAAAAAGGAGGTTTCATGATGAGCGGAAGAAAA‑AAAAAAAA‑GGCAGTGAAAAAAGTTTGAGAGATTGTTGCt                                                                   >  1:272135/1‑149 (MQ=255)
                                                                                        atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                               <  4:702260/151‑1 (MQ=255)
                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑AAAAAAAAATGGAGTTGGCATGATGAGCGGAAGGAAT‑AAACAAAA‑AGCAGAGAAAAAATTGACAGAGATTGTCGCTAACttt                                                             >  1:926373/1‑150 (MQ=255)
                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt                                                             <  1:1709629/150‑1 (MQ=255)
                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                            <  2:543051/151‑1 (MQ=255)
                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                            <  4:1217011/151‑1 (MQ=255)
                                                                                            aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                           <  4:197796/151‑1 (MQ=255)
                                                                                             cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTCGA‑AAAA‑AACAAAAAATTGAGGTGGCATGATGAGGGGAAAGAAA‑AAACAACACGGCGGGAAAAAAGTT‑AAAGAGATTTTTGCTaaactt                                                             >  3:545635/1‑144 (MQ=255)
                                                                                             cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑AGAATTTTTTCAAA‑CAAA‑GACAAAAAAAGGGGTTGCCCTGATGGGCGAAAGAAAT‑AAAAAACA‑AGCCAAGAAAAAGGTGACAGAGAATGTCGCTAACtt                                                              >  4:1569025/1‑147 (MQ=255)
                                                                                                tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTCCGA‑CAAA‑AAAAAAAAAATGAGTTGCCATGATTAGCGGAAATAAA‑AAAAAAAA‑AGCAGGGAATAAAGTGAAAGAGATTTTCGCCAAATTTTTCGcc                                                       >  4:391682/1‑151 (MQ=255)
                                                                                                tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGCATTTTTTCAAA‑AAAA‑AAAAAAAAACTGAGTTGCCAATTTTTGCGAAAGGAAT‑AAACAACA‑GGCCGAGAAAAACGTTAGAGAGAttttggcccac                                                                >  1:40462/1‑133 (MQ=255)
                                                                                                   cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑AACAAAAAATGGAGGTGGCATGTTGAGCGGAAGGAAT‑AAACAACA‑GGCAGGGGAAAAGTTTACAGAGATTGTCGCTAAATTTACCCCCCTg                                                    >  4:1320056/1‑151 (MQ=255)
                                                                                                    aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCAAGa                                                   <  2:15595/151‑1 (MQ=255)
                                                                                                    aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TAAATTTTTCCAAA‑CAAA‑TACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATg                                                    <  1:1829035/150‑1 (MQ=255)
                                                                                                    aaaaaaTAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                    <  1:91312/150‑1 (MQ=255)
                                                                                                     aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAA‑CAAA‑AACAAAAAATGGAGTTGGCATGGTGGGGGAAAGTAAT‑AAAAAACA‑AGCCGGGAATAAATTGACAGAGATTTTTGCCAAATTTATCGCCCTGa                                                   >  1:271467/1‑150 (MQ=255)
                                                                                                       aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TAAATTTTTCCAGA‑AAAA‑TAAAAAAACTGGATTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                                <  1:1071340/151‑1 (MQ=255)
                                                                                                                 ccACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                       <  4:914028/150‑1 (MQ=255)
                                                                                                                  cacaTTTTTAGTGGTATTTTGTGGCGG‑TGAATTTTTCCAGA‑AAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATg                                     <  4:1319641/151‑1 (MQ=255)
                                                                                                                  cacaTTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGA‑CAAA‑TAAAAAAAATGGGGGTGCCATGATGAGGGAAAGGAAT‑AAGCAAAA‑GGCAGGGAAAAAATTGACAGAGAATGTCGCTAACTTTTCCGCCCCGAttttttaccca                                        >  4:1485389/1‑140 (MQ=255)
                                                                                                                  cacaTTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGA‑CAAA‑AACAAAAAATTGGGTTGCCCTGATTAGCGGAAGTAAT‑AAAAAAAA‑AGCAGGGGATAAATTTACAAAGAATTTTGCTAACTTTAACGCCATGATTTTTAcccca                                        >  4:577930/1‑146 (MQ=255)
                                                                                                                     aTTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTCCGA‑CAAA‑AACAAAAAATGGGGGTGGCCTGGTGGGCGAAAGGAAA‑AAACAAAA‑AGCAGGGAAAAAAGTTAAAGAGAATGTTGCTAACTTTTTCGCCCTGAATTTTACCCCAATGcc                                   >  1:469314/1‑150 (MQ=255)
                                                                                                                     aTTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGA‑CCAA‑TAAAAAAAATGGAGGTGTCATGATGAGGGAAAGAAAT‑AAGCAAAA‑AGCAGGGGAAAAATTTACAGAGATTTTCGCTAACTTTTACGCCCTGATTTTTTCCCCCATGcc                                   >  4:252803/1‑150 (MQ=255)
                                                                                                                      tttttAGGGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAAT‑AAGCAACA‑GGCAGTAAATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATGATTTCTACCCGAATGc                                    <  1:1496806/148‑1 (MQ=255)
                                                                                                                         ttAGTGGTAGTTTGTGGCGG‑TGATTTTTCCCAGA‑AAAA‑AACAAAAACGGGAGTTGCCATGATGAGCAAAAGTAAT‑AAGAAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                               <  1:650871/150‑1 (MQ=255)
                                                                                                                          tAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAAT‑AAGCAACA‑GGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                              <  1:1734773/150‑1 (MQ=255)
                                                                                                                            gTGGTATTTTGTGGCGG‑TGAATTTTTCAAGA‑AAAA‑TACAAAAACTGGAGTTGCCATGATGAGAAAAAGTAAT‑AAGCAACA‑GGCAGTGAATAATTTGAAAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                            <  2:372579/150‑1 (MQ=255)
                                                                                                                                    ttGTGGGGG‑TGAATTTTTCCAGA‑AAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAA‑AGGCAACA‑GGCGGTGAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                    <  1:705619/150‑1 (MQ=255)
                                                                                                                                       tgGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                 >  3:256658/1‑150 (MQ=255)
                                                                                                                                        ggCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGGGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                 <  2:339898/149‑1 (MQ=255)
                                                                                                                                        ggCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc               >  4:1406135/1‑151 (MQ=255)
                                                                                                                                        ggCGG‑TAAATTTTTCCAGA‑AAAA‑TACAAAAACGGGAGTGGCAATGATGAGCGAAAGAAAT‑AAGAAACA‑GCCAGTGAATAAGTTGACAGATTTTGCGGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa                <  1:29189/150‑1 (MQ=255)
                                                                                                                                               gAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc         >  4:192829/1‑151 (MQ=255)
                                                                                                                                               gAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc         >  4:751613/1‑151 (MQ=255)
                                                                                                                                                aaTTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAAT‑AAGCAACA‑GGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa        <  2:413408/151‑1 (MQ=255)
                                                                                                                                                  tttttCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc      >  3:466103/1‑151 (MQ=255)
                                                                                                                                                      tCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa   >  4:841568/1‑150 (MQ=255)
                                                                                                                                                       ccAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa   >  3:292036/1‑149 (MQ=255)
                                                                                                                                                       ccAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGAAACA‑GGCAGTGATTAAGTTGACAAAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  4:1801494/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                         
CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGA‑CAAA‑TACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACA‑GGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206285‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑