Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,630,4090TC49.0% 11.9 / inf 259E65G (GAA→GGA) yhiIputative membrane fusion protein (MFP) of efflux pump
Reads supporting (aligned to +/- strand):  ref base T (54/78);  new base C (66/61);  total (120/139)
Fisher's exact test for biased strand distribution p-value = 8.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01

Only 100 of 262 total aligned reads displayed.

GTTTCGCTTTGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAG  >  NC_000913/3630259‑3630557
                                                                                                                                                      |                                                                                                                                                    
gTTTCGCTTTGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCctt                                                                                                                                                      <  4:256991/151‑1 (MQ=255)
    cGCTTTGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCttt                                                                                                                                                  <  3:153160/151‑1 (MQ=255)
     gCTTTGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTc                                                                                                                                                 <  4:1358123/151‑1 (MQ=255)
      cTTTGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTc                                                                                                                                                 <  1:797776/150‑1 (MQ=255)
        ttGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCa                                                                                                                                                <  1:1181348/149‑1 (MQ=255)
        ttGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCa                                                                                                                                                <  4:1673297/149‑1 (MQ=255)
        ttGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCAcc                                                                                                                                              >  4:442772/1‑151 (MQ=255)
          gTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCa                                                                                                                                             <  4:1100353/150‑1 (MQ=255)
          gTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCa                                                                                                                                             <  1:1751111/150‑1 (MQ=255)
          gTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCa                                                                                                                                             <  2:404481/150‑1 (MQ=255)
           tCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCAcc                                                                                                                                              >  2:378148/1‑148 (MQ=255)
             gTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAAt                                                                                                                                         <  3:327224/151‑1 (MQ=255)
             gTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAAt                                                                                                                                         <  4:1276462/151‑1 (MQ=255)
                gCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGgt                                                                                                                                      <  4:377993/151‑1 (MQ=255)
                      gCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGAt                                                                                                                                >  4:1347236/1‑151 (MQ=255)
                       cAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATa                                                                                                                               <  4:1192724/151‑1 (MQ=255)
                       cAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGAt                                                                                                                                >  1:202103/1‑150 (MQ=255)
                        aaaGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATAc                                                                                                                              >  1:253932/1‑151 (MQ=255)
                        aaaGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATAc                                                                                                                              >  1:102978/1‑151 (MQ=255)
                            ccTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGccc                                                                                                                          <  4:213957/151‑1 (MQ=255)
                              tGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCgg                                                                                                                        >  4:1250862/1‑151 (MQ=255)
                                cgcgGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCa                                                                                                                      <  1:376256/151‑1 (MQ=255)
                                 gcgGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCaa                                                                                                                     <  4:1054565/151‑1 (MQ=255)
                                    gcagcaACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAAttt                                                                                                                  <  4:924333/151‑1 (MQ=255)
                                    gcagcaACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAAttt                                                                                                                  <  1:1210922/151‑1 (MQ=255)
                                     cagcaACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAAtttt                                                                                                                 <  4:512843/151‑1 (MQ=255)
                                        caACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCt                                                                                                              >  3:109213/1‑151 (MQ=255)
                                         aaCGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTg                                                                                                             <  3:296125/151‑1 (MQ=255)
                                          aCGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTgg                                                                                                            >  1:1495343/1‑151 (MQ=255)
                                            ggCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCa                                                                                                          <  1:1250334/151‑1 (MQ=255)
                                              cgcTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAat                                                                                                        <  1:539799/151‑1 (MQ=255)
                                              cgcTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCaa                                                                                                         >  3:271234/1‑150 (MQ=255)
                                                 tttGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATc                                                                                                     >  2:5526/1‑151 (MQ=255)
                                                  ttGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATcc                                                                                                    >  4:550053/1‑151 (MQ=255)
                                                  ttGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATc                                                                                                     <  4:1416497/150‑1 (MQ=255)
                                                   tgtgCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATcc                                                                                                    <  3:212672/150‑1 (MQ=255)
                                                    gtgCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCa                                                                                                   <  1:1099930/150‑1 (MQ=255)
                                                    gtgCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCa                                                                                                   <  4:1142555/150‑1 (MQ=255)
                                                    gtgCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCAc                                                                                                  <  2:335023/151‑1 (MQ=255)
                                                    gtgCCTCTTTGATTTGAGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCAc                                                                                                  >  4:1090728/1‑151 (MQ=255)
                                                      gCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACtt                                                                                                <  1:782857/151‑1 (MQ=255)
                                                      gCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACt                                                                                                 <  2:189451/150‑1 (MQ=255)
                                                         tctTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCg                                                                                             >  2:253441/1‑151 (MQ=255)
                                                              gATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGc                                                                                         <  4:1337632/150‑1 (MQ=255)
                                                                  tGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCa                                                                                     <  4:1718152/150‑1 (MQ=255)
                                                                   gcgcGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAAt                                                                                   <  1:1550364/151‑1 (MQ=255)
                                                                   gcgcGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAAt                                                                                   >  4:358514/1‑151 (MQ=255)
                                                                   gcgcGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAAt                                                                                   <  1:1681748/151‑1 (MQ=255)
                                                                    cgcgATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCa                                                                                     <  1:1346686/148‑1 (MQ=255)
                                                                            cTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCAtt                                                                          >  4:1812056/1‑151 (MQ=255)
                                                                             ttCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCAtt                                                                          >  1:993208/1‑150 (MQ=255)
                                                                               ccAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACt                                                                       >  4:162219/1‑151 (MQ=255)
                                                                               ccAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACt                                                                       <  1:140081/151‑1 (MQ=255)
                                                                               ccAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACt                                                                       <  3:501183/151‑1 (MQ=255)
                                                                                    cGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAg                                                                  <  4:1861576/151‑1 (MQ=255)
                                                                                    cGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGAc                                                                    >  1:1600528/1‑149 (MQ=255)
                                                                                     gCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGc                                                                 <  1:647365/151‑1 (MQ=255)
                                                                                     gCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGc                                                                 <  2:64116/151‑1 (MQ=255)
                                                                                        gTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCaaa                                                              <  4:482551/151‑1 (MQ=255)
                                                                                             tGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCtt                                                         >  1:940959/1‑151 (MQ=255)
                                                                                              gCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTc                                                        >  1:517842/1‑151 (MQ=255)
                                                                                                aaCACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCg                                                      <  1:1530284/151‑1 (MQ=255)
                                                                                                 acacGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCgg                                                     >  3:3501/1‑151 (MQ=255)
                                                                                                 acacGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCgg                                                     >  4:8351/1‑151 (MQ=255)
                                                                                                  cacGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGc                                                    <  4:795015/151‑1 (MQ=255)
                                                                                                  cacGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGc                                                    >  4:760263/1‑151 (MQ=255)
                                                                                                    cgcgAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGcac                                                  >  3:225912/1‑151 (MQ=255)
                                                                                                     gcgAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGca                                                   >  4:563560/1‑149 (MQ=255)
                                                                                                         gTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTg                                             <  3:424900/151‑1 (MQ=255)
                                                                                                           aTCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGc                                            <  4:1282638/150‑1 (MQ=255)
                                                                                                           aTCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGcc                                           >  2:124995/1‑151 (MQ=255)
                                                                                                              cATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCggg                                        >  4:1601469/1‑151 (MQ=255)
                                                                                                              cATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCggg                                        >  2:168156/1‑151 (MQ=255)
                                                                                                                tCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACACCAAAGCCTTCCGGCACAcc                                               >  4:532557/1‑142 (MQ=255)
                                                                                                                   tCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGgcgc                                     <  4:1790284/149‑1 (MQ=255)
                                                                                                                     gCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACa                                 <  3:384215/151‑1 (MQ=255)
                                                                                                                     gCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAAc                                  >  2:80041/1‑150 (MQ=255)
                                                                                                                     gCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACa                                 <  1:1888749/151‑1 (MQ=255)
                                                                                                                     gCCAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACa                                 <  3:46579/151‑1 (MQ=255)
                                                                                                                       cAGCACTTCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGc                               <  4:419358/151‑1 (MQ=255)
                                                                                                                          cACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGccac                            >  2:507273/1‑151 (MQ=255)
                                                                                                                              tCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGccacca                          <  2:298626/149‑1 (MQ=255)
                                                                                                                              tCACCTTCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCcaccac                         <  1:442614/150‑1 (MQ=255)
                                                                                                                                  cTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCAccacgccac                    >  1:128206/1‑151 (MQ=255)
                                                                                                                                   ttCGCGAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCAccacgccacg                   >  4:620772/1‑151 (MQ=255)
                                                                                                                                    tCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCAccacgccacg                   >  4:1534815/1‑150 (MQ=255)
                                                                                                                                    tCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCAccacgccac                    <  4:1580777/149‑1 (MQ=255)
                                                                                                                                     cgcgAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGAt                 >  3:405788/1‑151 (MQ=255)
                                                                                                                                       cgAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGAt                 >  3:65475/1‑149 (MQ=255)
                                                                                                                                       cgAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAg               <  1:601140/151‑1 (MQ=255)
                                                                                                                                       cgAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGAt                 >  1:82782/1‑149 (MQ=255)
                                                                                                                                        gAACAAACTTGCCTTCTTTCTCCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAg               <  1:687834/150‑1 (MQ=255)
                                                                                                                                        gAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAgc              >  1:1012933/1‑151 (MQ=255)
                                                                                                                                        gAACAAACTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAgc              >  4:324413/1‑151 (MQ=255)
                                                                                                                                          aCAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAgccg            >  4:667063/1‑151 (MQ=255)
                                                                                                                                            aaaCTTGCCTTATTTCTCCAGAATGGTGTCGATACCCCGGGTAATTTTGCTGTCAATATCCCCTTCCGTCGCTTCAATGCGCCTATTACTGACAGTAAAGCCTTCGGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAgccgcc          <  1:1639518/151‑1 (MQ=255)
                                                                                                                                             aaCTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgcca         <  1:1302992/151‑1 (MQ=255)
                                                                                                                                             aaCTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgcca         <  1:410645/151‑1 (MQ=255)
                                                                                                                                              aCTTGCCTCCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgccac        >  4:287738/1‑151 (MQ=255)
                                                                                                                                               cTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGccgccacc       >  4:1700175/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
GTTTCGCTTTGTCGTTGCTCCAGCAAAGCCTGCGCGGCAGCAACGGCGCTTTGTGCCTCTTTGATTTGCGCGATGGCTTCCAGTCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAG  >  NC_000913/3630259‑3630557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑