Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,660,4550AG49.8% 16.3 / inf 279V14V (GTA→GTG) mdtFanaerobic multidrug efflux transporter, ArcA‑regulated
Reads supporting (aligned to +/- strand):  ref base A (86/54);  new base G (72/67);  total (158/121)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 280 total aligned reads displayed.

CGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCGGTCACTCAG  >  NC_000913/3660305‑3660602
                                                                                                                                                      |                                                                                                                                                   
   tttGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACtt                                                                                                                                                  >  1:416247/1‑151 (MQ=255)
   tttGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCtt                                                                                                                                                  >  1:1498970/1‑151 (MQ=255)
     tGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTTCCTGGGTACTTGc                                                                                                                                                >  4:622269/1‑151 (MQ=255)
        aaCGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCat                                                                                                                                             >  1:603683/1‑151 (MQ=255)
         aCGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCatt                                                                                                                                            <  1:647072/151‑1 (MQ=255)
         aCGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCatt                                                                                                                                            <  4:1396567/151‑1 (MQ=255)
         aCGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCatt                                                                                                                                            <  1:129779/151‑1 (MQ=255)
          cGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCatt                                                                                                                                            >  2:534068/1‑150 (MQ=255)
           gCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCattat                                                                                                                                          <  2:29135/151‑1 (MQ=255)
             aTTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCattatt                                                                                                                                         >  3:202710/1‑150 (MQ=255)
              ttCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATtattat                                                                                                                                       >  4:817266/1‑151 (MQ=255)
              ttCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATtattat                                                                                                                                       >  1:343753/1‑151 (MQ=255)
               tCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATtattat                                                                                                                                       <  1:598108/150‑1 (MQ=255)
                cGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTatg                                                                                                                                      >  1:726604/1‑150 (MQ=255)
                cGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTatga                                                                                                                                     <  4:1043538/151‑1 (MQ=255)
                 gTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTatgat                                                                                                                                    <  1:169522/151‑1 (MQ=255)
                    cGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCt                                                                                                                                 <  4:217671/151‑1 (MQ=255)
                    cGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCt                                                                                                                                 <  1:1268080/151‑1 (MQ=255)
                     gggTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCtt                                                                                                                                >  4:1073802/1‑151 (MQ=255)
                       gTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGc                                                                                                                              <  4:1679145/151‑1 (MQ=255)
                          tCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAgg                                                                                                                           >  1:626863/1‑151 (MQ=255)
                          tCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAgg                                                                                                                           >  1:468130/1‑151 (MQ=255)
                            aaaGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAggtg                                                                                                                         >  1:293731/1‑151 (MQ=255)
                              aGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAggtggt                                                                                                                       <  4:126567/151‑1 (MQ=255)
                                cACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCt                                                                                                                     >  4:444651/1‑151 (MQ=255)
                                 aCGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTg                                                                                                                    <  4:1322305/151‑1 (MQ=255)
                                     gCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGa                                                                                                                <  4:1056352/151‑1 (MQ=255)
                                     gCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCg                                                                                                                 >  2:14500/1‑150 (MQ=255)
                                     gCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGa                                                                                                                <  1:850078/151‑1 (MQ=255)
                                      cAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGAt                                                                                                               >  4:318701/1‑151 (MQ=255)
                                      cAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGAt                                                                                                               >  4:919038/1‑151 (MQ=255)
                                       aaTTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATc                                                                                                              <  3:324930/151‑1 (MQ=255)
                                          ttCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATg                                                                                                           >  4:1506206/1‑151 (MQ=255)
                                          ttCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCAt                                                                                                            <  2:495391/150‑1 (MQ=255)
                                           tcctccAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATg                                                                                                           <  1:543980/150‑1 (MQ=255)
                                            cctccAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGa                                                                                                          >  1:760748/1‑150 (MQ=255)
                                              tccAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACt                                                                                                       >  4:979077/1‑151 (MQ=255)
                                                 agccagGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGCCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTAc                                                                                                    >  1:941490/1‑151 (MQ=255)
                                                  gccagGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTAcc                                                                                                   <  4:1121917/151‑1 (MQ=255)
                                                    cagGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCgg                                                                                                 >  1:173549/1‑151 (MQ=255)
                                                     agGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGt                                                                                                <  4:838369/151‑1 (MQ=255)
                                                            cGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTgcgc                                                                                           >  4:700324/1‑149 (MQ=255)
                                                            cGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAg                                                                                         <  4:1819045/151‑1 (MQ=255)
                                                             gCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGt                                                                                        >  1:810511/1‑151 (MQ=255)
                                                              ccAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGt                                                                                        >  3:381594/1‑150 (MQ=255)
                                                               cAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTAt                                                                                      >  4:725161/1‑151 (MQ=255)
                                                                    ccGAATCGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCa                                                                                 <  1:451205/151‑1 (MQ=255)
                                                                          cGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTg                                                                            <  1:819322/150‑1 (MQ=255)
                                                                          cGAAACAATAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGAACGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTgc                                                                           >  4:46757/1‑151 (MQ=255)
                                                                           gAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTgcg                                                                          >  4:538494/1‑151 (MQ=255)
                                                                            aaaCAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTgcgc                                                                         >  4:543232/1‑151 (MQ=255)
                                                                                aaTAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCAcc                                                                     <  4:790462/151‑1 (MQ=255)
                                                                                  tAACGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCg                                                                    >  4:1169965/1‑150 (MQ=255)
                                                                                     cGTTGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCa                                                                <  1:1826344/151‑1 (MQ=255)
                                                                                       ttGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCAtt                                                              >  1:1149568/1‑151 (MQ=255)
                                                                                       ttGCAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCAtt                                                              >  4:123591/1‑151 (MQ=255)
                                                                                       ttGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCAtt                                                              >  4:1304076/1‑151 (MQ=255)
                                                                                         gCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTAc                                                            >  3:273952/1‑151 (MQ=255)
                                                                                          cAGGCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTAcc                                                           >  1:1202433/1‑151 (MQ=255)
                                                                                          cAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTAcc                                                           >  4:1716125/1‑151 (MQ=255)
                                                                                             gCTTAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTc                                                        <  1:217139/151‑1 (MQ=255)
                                                                                             gCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTc                                                        <  1:1091938/151‑1 (MQ=255)
                                                                                              cTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTc                                                        >  1:26902/1‑150 (MQ=255)
                                                                                               ttAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTc                                                        >  4:1080934/1‑149 (MQ=255)
                                                                                                tAAGGGGGCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCa                                                       <  2:401515/149‑1 (MQ=255)
                                                                                                tAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAgc                                                     >  2:199571/1‑151 (MQ=255)
                                                                                                tAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAg                                                      <  2:379494/150‑1 (MQ=255)
                                                                                                      ggcTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTAcc                                               >  4:1126654/3‑151 (MQ=255)
                                                                                                          ttCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTa                                             <  4:1369447/149‑1 (MQ=255)
                                                                                                          ttCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTAt                                            <  3:69950/150‑1 (MQ=255)
                                                                                                          ttCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATc                                           >  4:1640925/1‑151 (MQ=255)
                                                                                                            cATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATcc                                          >  1:1041269/1‑150 (MQ=255)
                                                                                                                  tAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGcc                                   >  4:292222/1‑151 (MQ=255)
                                                                                                                   aaCTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGcc                                   <  4:1307474/150‑1 (MQ=255)
                                                                                                                   aaCTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCg                                  <  2:541815/151‑1 (MQ=255)
                                                                                                                    aCTATTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCg                                  >  4:20367/1‑150 (MQ=255)
                                                                                                                    aCTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCg                                  >  2:332130/1‑150 (MQ=255)
                                                                                                                    aCTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCg                                  >  4:1782142/1‑150 (MQ=255)
                                                                                                                       aTTTTATTGATCGCCCGGTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTg                                     >  3:531936/1‑144 (MQ=255)
                                                                                                                         tttATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATgcgc                            >  1:1004248/1‑151 (MQ=255)
                                                                                                                           tATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCa                           >  2:228474/1‑150 (MQ=255)
                                                                                                                             ttGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAAc                        >  1:1246564/1‑151 (MQ=255)
                                                                                                                              tGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACg                       >  4:1021815/1‑151 (MQ=255)
                                                                                                                               gATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACgg                      >  1:1393202/1‑151 (MQ=255)
                                                                                                                                aTCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGt                     >  1:1574471/1‑151 (MQ=255)
                                                                                                                                   gCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGt                     >  3:176191/1‑148 (MQ=255)
                                                                                                                                     ccGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTaga                  >  4:389399/1‑149 (MQ=255)
                                                                                                                                      cGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTaga                  >  3:117294/1‑148 (MQ=255)
                                                                                                                                      cGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTaga                  >  1:1648966/1‑148 (MQ=255)
                                                                                                                                        gTTTTTGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACt             <  4:1774380/151‑1 (MQ=255)
                                                                                                                                          ttttGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCg           >  1:1156388/1‑151 (MQ=255)
                                                                                                                                           tttGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCg           >  3:490161/1‑150 (MQ=255)
                                                                                                                                           tttGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCgg          >  1:1717287/1‑151 (MQ=255)
                                                                                                                                            ttGCCTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCgg          <  4:1477165/150‑1 (MQ=255)
                                                                                                                                            ttGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCgg          <  1:1333205/150‑1 (MQ=255)
                                                                                                                                            ttGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCGGt         >  1:366474/1‑151 (MQ=255)
                                                                                                                                              gCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCGGTc        <  1:1886212/150‑1 (MQ=255)
                                                                                                                                               ccTGGGTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCGGTCAc      <  2:461583/151‑1 (MQ=255)
                                                                                                                                                  gggTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCGGTCACTCa   <  3:249353/151‑1 (MQ=255)
                                                                                                                                                   ggTGCTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCGGTCACTCAg  <  3:390522/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
CGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCTCCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGTCAGCGCTACCTATCCAGGTGCCGATGCGCAAACGGTAGAAGACTCGGTCACTCAG  >  NC_000913/3660305‑3660602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑