Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,7910AC41.1% 271.2 / 29.5 141V161G (GTG→GGG) basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base A (72/11);  new base C (0/58);  total (72/69)
Fisher's exact test for biased strand distribution p-value = 8.91e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

ACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGC  >  NC_000913/4332641‑4332940
                                                                                                                                                        |                                                                                                                                                     
aCATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCa                                                                                                                                                       <  1:1245941/151‑1 (MQ=255)
aCATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCa                                                                                                                                                       <  3:180248/151‑1 (MQ=255)
 cATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCAc                                                                                                                                                      >  1:680486/1‑151 (MQ=255)
 cATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCAc                                                                                                                                                      >  2:56249/1‑151 (MQ=255)
   tGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCAccc                                                                                                                                                    >  1:535908/1‑151 (MQ=255)
    gTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCAcccc                                                                                                                                                   <  1:1695886/151‑1 (MQ=255)
      tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCAccccc                                                                                                                                                  <  1:1710787/150‑1 (MQ=255)
       gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGc                                                                                                                                                >  1:1823636/1‑151 (MQ=255)
       gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGc                                                                                                                                                >  4:1496277/1‑151 (MQ=255)
       gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGc                                                                                                                                                >  4:1790975/1‑151 (MQ=255)
        aTAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGcc                                                                                                                                               <  4:1574078/151‑1 (MQ=255)
        aTAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGcc                                                                                                                                               >  4:553260/1‑151 (MQ=255)
        aTAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGcc                                                                                                                                               <  4:1308207/151‑1 (MQ=255)
             tACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTgg                                                                                                                                          >  2:104143/1‑151 (MQ=255)
                   aagaaAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCg                                                                                                                                    >  4:1833546/1‑151 (MQ=255)
                    agaaAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGc                                                                                                                                   >  4:676723/1‑151 (MQ=255)
                       aaaTGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGt                                                                                                                                >  2:39107/1‑151 (MQ=255)
                       aaaTGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGt                                                                                                                                >  1:757843/1‑151 (MQ=255)
                         aTGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTc                                                                                                                              >  4:286351/1‑151 (MQ=255)
                          tGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCg                                                                                                                             >  2:210570/1‑151 (MQ=255)
                            aCTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTg                                                                                                                           >  1:1005988/1‑151 (MQ=255)
                               gTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTgcgc                                                                                                                        >  2:161409/1‑151 (MQ=255)
                                tCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGcgcg                                                                                                                       >  4:1253857/1‑151 (MQ=255)
                                 ccGGCCCGCGCCGGTTGCAGCGGCGGGAAGCCCCTCCCCATCATCTGATCAAGCCGTCCCACAAACGGTGCTACATCAATGTGTTGCTTTTCCGCCAGCGGTTCA‑AGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTgcgc                                                                                                                        <  1:1214695/149‑1 (MQ=255)
                                 ccGGCCCGCGCCGGTGGCGGCGGGGGGGAGCCCCCCCCCATCTTCTGACCAGCCCGTCCCACTAACGGTGCTACATCAATGTGAGGGTTTTCCGCCAGCTGTTCA‑AGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTc                                                                                                                              <  1:1796371/143‑1 (MQ=255)
                                 ccGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGcgcg                                                                                                                       >  4:539677/1‑150 (MQ=255)
                                 ccGCCACGCCCCAGTTGCAGCAGCGGGGAGCCGCTCTCCATCATCTGACCAGGCCGTCCCACTACCGGTGCTACATAAATGTGTTGCTTTTTCGCCA‑CAATTCCAAGAGGAAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGt                                                                                                                            <  2:173587/145‑1 (MQ=255)
                                     cccgccCCAGTTGCAGCAGCGGGGAGCCGCTCTCCATCATCTGATAAAGCCGTGCCACAAACGGTGCTACATCAATGTGTTGCTTTTCCGCCAGCAGTTCC‑AGAAGCAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc                                                                                                                  <  1:1640539/149‑1 (MQ=255)
                                     cACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc                                                                                                                  >  3:104264/1‑151 (MQ=255)
                                           ccattggcagaaGTTGGGAGCCGCTCTCCATCATCTGATCAGCCCGTCCCACTAACGGTGCTACATAAATGTGATGCTTTTCCGCCAGCATTCCC‑AGATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGAc                                                                                                                     <  4:1734796/136‑1 (MQ=255)
                                           ccAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGt                                                                                                            >  3:254817/1‑151 (MQ=255)
                                           ccAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGt                                                                                                            >  4:1600008/1‑151 (MQ=255)
                                            cggTTGCAGCAGGGGGGAGCCCCTCCCCATCATCTGATAAAGCCGTCCCACTAACGGTGCTACACCAATGTGATGTTTTTTCGCCAGCAGTCCC‑AGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTa                                                                                                           <  1:874876/149‑1 (MQ=255)
                                            cAGTTGCAGCGGCGGGGGACCCCCCCCCATTATCTGACAAAGCCGCGCCACAACCGGTCCTACATCATTGTGTTTCTTTTTCGCCAGCATTCCC‑AAATGAAAT‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc                                                                                                                  <  2:323768/144‑1 (MQ=255)
                                            cAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGt                                                                                                            >  1:1392869/1‑150 (MQ=255)
                                                 gcagcaGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaaca                                                                                                       >  1:1167781/1‑150 (MQ=255)
                                                      ggtggggAGCCGCTCTCCATCTTCTGACCAAGCCGTCCCACTACCGTTGCTACATCATTGTGATGCTTTTCCCCCAGCATTTCC‑AGAGCAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaa                                                                                                      <  4:1830180/142‑1 (MQ=255)
                                                       gggggggGACCCTCTCCATCATCTGATCAAGCCGTGCCACTACCGGTGATACATCAATGTGTTGCGTTTTCGCCGGCAGTCCC‑AGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCttt                                                                                                 <  3:76836/147‑1 (MQ=255)
                                                       gcTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTc                                                                                                >  4:602073/1‑151 (MQ=255)
                                                         tGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGt                                                                                              >  4:1180010/1‑151 (MQ=255)
                                                         ggggAGCCGCTCTCCATCATCTGATCAAGCCGTCCCACTAACGGTGCTACATAAATGTGATGCTTTTTCGCCAGCAGTCCC‑AGATGCAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGt                                                                                              <  4:1773955/150‑1 (MQ=255)
                                                          gggAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGtt                                                                                             >  1:1430301/1‑151 (MQ=255)
                                                          gggAGACGCTCTCCATCATCTGAACAGGCCGTGCCACTAGGGGTGCTCCATAAATGTGGTGCTTTTCCGCCGGCAGTTCC‑AGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGtt                                                                                             <  3:447439/151‑1 (MQ=255)
                                                            gagaCGCTCCCCATCATCTGACCAAGCCGGCCCACTAACGGTGCTACATAAATGTGTTGCTTTTCCGCCAGCAGTCCC‑AAATAAAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTAt                                                                                           <  1:1751686/151‑1 (MQ=255)
                                                             agaCGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATc                                                                                          >  1:1738280/1‑151 (MQ=255)
                                                                ccctccCCATCATCTGATCAAGCCGTGCCACTACCGGTGCTACATAAATGTGATGCTTTTTCGCCAGCAGTCCA‑AGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCa                                                                                        <  4:438010/148‑1 (MQ=255)
                                                                ccctccCCATCATCTGACCAGCCCGTCCCACTACCGGTTCTACATAATTGTGATGCTTTTCCGCCAGCAGTTCC‑AGATGAAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCa                                                                                        <  1:1551230/148‑1 (MQ=255)
                                                                    ctcCATCATCTAATCAAGCCGCGCCACAACCGGTGCAACATCAATGTGTTGCTTTTCCGCCAGCATTCCC‑AGATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg                                                                                       <  4:1459263/147‑1 (MQ=255)
                                                                    ccccATCATCTGACCAAGCCGTCCCACTAACGGTCATACATAAATGTGATGCTTTTTCGCCAGCAGTCCC‑AGATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGt                                                                                    <  4:1561446/148‑1 (MQ=255)
                                                                      ccATCTTCTGACCAAGCCGTGCCACTAAGGGTGCTACATCAATGTGATGCGTTTCCGCCAGCATTTCC‑AGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCt                                                                                 <  4:1034015/151‑1 (MQ=255)
                                                                          catcTGATAAACCCGTGCCACTAGCGGTGCTACATCAATGTGGTGCGTTTCCCCCAGCGTTTCC‑AGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTg                                                                                <  3:9586/148‑1 (MQ=255)
                                                                            tcTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTc                                                                             >  1:143082/1‑149 (MQ=255)
                                                                                 tCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGAc                                                                      >  4:1915784/1‑151 (MQ=255)
                                                                                   aaGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg                                                                    >  4:149564/1‑151 (MQ=255)
                                                                                   aaGCCGTCCCATTAACGGTGCTACATAATTGTGATGCGTTTCCCCCAGCGGTCCA‑AGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCg                                                                        <  1:806352/147‑1 (MQ=255)
                                                                                      ccGTGCCATAAGCGGTGCTACATCAATGTGATGCGTTTCCGCCAGAGGTTCA‑AGATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                                 <  4:810646/151‑1 (MQ=255)
                                                                                      ccGTGCCACAAACGGTGCTACATCAATGTGATGCGTTTCCGCCAGCAGTTCC‑AGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGa                                                                   <  4:1060913/149‑1 (MQ=255)
                                                                                      ccGTGCAACAACGGGTGCAAAATCAGTGGGGTGAGTTTTCGCCGACAGTCCC‑AAACGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg                                                                    <  1:101968/148‑1 (MQ=255)
                                                                                           ccACGAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGGTATCCAGCGTGCTGGTCAGGCGACTg                                                                    >  1:799521/1‑143 (MQ=255)
                                                                                           ccACAAACGGTGCTACATCAATGTGATGCTTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CCCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGa                                                              <  4:51951/149‑1 (MQ=255)
                                                                                            cACTAAGGGTGCTACATCAATGTGATGCTTTTTCGCCAGCAGTTCC‑AGATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATc                                                            <  1:552032/150‑1 (MQ=255)
                                                                                             aCTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGt                                                          >  4:705889/1‑151 (MQ=255)
                                                                                                accGGTGCTACATCAATGTGATGTGTTTTCCCCAGCAGTTCC‑AGAGGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCg                                                           <  4:913833/145‑1 (MQ=255)
                                                                                                aaCGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGttt                                                        >  1:186694/1‑150 (MQ=255)
                                                                                                aaCGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGttt                                                        >  1:600934/1‑150 (MQ=255)
                                                                                                 aCGGTGCTACATCAATGTGATGTTTTTCCCCCAGCAGTTCC‑AGAGGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCg                                                           <  1:1019860/146‑1 (MQ=255)
                                                                                                 aCGGTGCTACATCAATGTGATGCTTTTCCGCCAGCATTTCC‑AGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTa                                                       <  4:1111448/150‑1 (MQ=255)
                                                                                                    gTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCAGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc                                                   >  1:681205/1‑151 (MQ=255)
                                                                                                      gCTACATAAATGTGATGCTTTTTCGCAAGCAGTTCC‑AGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCg                                                 <  2:502009/151‑1 (MQ=255)
                                                                                                          cATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaaa                                              <  1:959996/150‑1 (MQ=255)
                                                                                                          cATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAac                                             >  3:484214/1‑151 (MQ=255)
                                                                                                          cATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAac                                             >  1:43595/1‑151 (MQ=255)
                                                                                                          cATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAac                                             >  2:497552/1‑151 (MQ=255)
                                                                                                           ataattgggATGCTTTTTCCCCAGC‑GGTCC‑AGGACCAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc                                                   <  1:111035/136‑1 (MQ=255)
                                                                                                           agaagtgggttgatttttGGCAAGCGCTTCA‑AGATGAAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGa                                                <  4:491904/133‑1 (MQ=255)
                                                                                                           aTCAATGTGATGCGTTTCCGCCAGCATTTCC‑AGAGGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaaa                                              <  2:412266/149‑1 (MQ=255)
                                                                                                             cAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAaccac                                          >  2:127800/1‑151 (MQ=255)
                                                                                                                tgtgTTGCTTTTCCCCCAGCATTCCC‑AGATGAAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacca                                           <  1:140136/147‑1 (MQ=255)
                                                                                                                tgtgATGCGTTTTCGACAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGc                                       >  2:446057/1‑151 (MQ=255)
                                                                                                                      gCGTTTGCGCCAGCAGTCCC‑AGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCg                                   <  1:1803726/149‑1 (MQ=255)
                                                                                                                       ctttttCGCCAGCAGTCCC‑AGATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGa                                  <  1:949602/147‑1 (MQ=255)
                                                                                                                        gTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAttt                               >  1:874408/1‑151 (MQ=255)
                                                                                                                         ttttCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTc                              >  4:174886/1‑151 (MQ=255)
                                                                                                                         ttttCGCCACCGGTTCC‑AGATCCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAtt                                <  2:107354/149‑1 (MQ=255)
                                                                                                                          ttccGCCAGCAGTTCC‑AGAGGAAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTc                              <  1:1422737/147‑1 (MQ=255)
                                                                                                                                 agcagTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTgg                      >  3:284913/1‑151 (MQ=255)
                                                                                                                                    agTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcgc                   >  1:135754/1‑151 (MQ=255)
                                                                                                                                    agTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcgc                   >  1:1356416/1‑151 (MQ=255)
                                                                                                                                     gTTCC‑AGATGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCGGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGt                        <  4:173209/145‑1 (MQ=255)
                                                                                                                                      ttCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACACCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtg                 >  4:781620/1‑151 (MQ=255)
                                                                                                                                         c‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgtg               <  1:1778497/150‑1 (MQ=255)
                                                                                                                                           aGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGa              >  4:1170669/1‑150 (MQ=255)
                                                                                                                                             aTGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATg           >  1:1602865/1‑151 (MQ=255)
                                                                                                                                              tGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATgg          >  4:1892617/1‑151 (MQ=255)
                                                                                                                                              ataaacccGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCt                  <  1:274601/137‑1 (MQ=255)
                                                                                                                                                 aaa‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGc         <  4:754091/149‑1 (MQ=255)
                                                                                                                                                 aaa‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCa        >  1:573877/1‑150 (MQ=255)
                                                                                                                                                  aa‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAAt      >  4:459/1‑151 (MQ=255)
                                                                                                                                                     cGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATg     <  4:1631970/150‑1 (MQ=255)
                                                                                                                                                       cACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGc  >  1:225639/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
ACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCC‑AGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGC  >  NC_000913/4332641‑4332940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑