Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,506,0280AC43.7% 261.4 / 10.1 136V18G (GTA→GGA) ydcOBenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base A (61/15);  new base C (0/59);  total (61/75)
Fisher's exact test for biased strand distribution p-value = 1.84e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGG  >  NC_000913/1505879‑1506175
                                                                                                                                                      |                                                                                                                                                    
gTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATAc                                                                                                                                                     <  1:602864/151‑1 (MQ=255)
  aCGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATAcc                                                                                                                                                    <  4:645652/150‑1 (MQ=255)
   cgcgATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGc                                                                                                                                                  >  1:1483114/1‑151 (MQ=255)
    gcgATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGcc                                                                                                                                                 <  2:359497/151‑1 (MQ=255)
          ccATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGCCAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑c                                                                                                                                          <  1:230899/151‑1 (MQ=255)
          ccATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGaaa                                                                                                                                            <  4:1620160/150‑1 (MQ=255)
          ccATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑c                                                                                                                                          >  3:418323/1‑151 (MQ=255)
          ccATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑c                                                                                                                                          >  1:1687597/1‑151 (MQ=255)
           cATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAG‑CCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTTCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑ccc                                                                                                                                        >  1:557580/1‑151 (MQ=255)
            aTAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑ccc                                                                                                                                        <  4:1868551/151‑1 (MQ=255)
             tAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCAGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑cccc                                                                                                                                       >  1:1303393/1‑151 (MQ=255)
                tGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGcc                                                                                                                                    >  1:1043583/1‑151 (MQ=255)
                 gTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATCCCGCCAGAAA‑CCCCGCCa                                                                                                                                   <  1:493520/151‑1 (MQ=255)
                 gTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGcc                                                                                                                                    <  4:1888467/150‑1 (MQ=255)
                      agTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGc                                                                                                                              >  2:98086/1‑151 (MQ=255)
                         cAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCgtg                                                                                                                           >  1:286070/1‑151 (MQ=255)
                         cAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCgtg                                                                                                                           >  4:344310/1‑151 (MQ=255)
                          aGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCgtgg                                                                                                                          >  4:315739/1‑151 (MQ=255)
                               acggACCCCCTTCGCCACCCCCGGCGCCTTCCCCCAGCAAGAATTTTGGGCGGTGGTGCCCCGGGCGACAATCGCTCCTTCCCATATTTTGGCCGTTGACCTGGGG‑TGACAAATAAATCCCCCAAAAAC‑CCCCGCCAGTAGCGTGGGTgga                                                                                                                     <  1:1084289/148‑1 (MQ=255)
                                 tGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTggagg                                                                                                                   >  4:451391/1‑151 (MQ=255)
                                    cGCCCTTCGCCACCCCCAGCGCCGCCACCCAGCAGGAGATTTGGGCGGGGGTGCCTCGGGCGACAACCGCTCCTTGCAATATTTTCGCCGCTGAACGGGCG‑TAGCAAATAAATACCCCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAAt                                                                                                                <  2:162180/151‑1 (MQ=255)
                                      cccttgcccACCCCCAGCGCCGTCACCCAGCCAGATTTTTGGGCGGTGGGGGCCCGGGCGACAATCGCTGCTTGCAAGATTATTGCCGCTGAAGGGGGG‑TAGCAAATAAATCCCGCCAAAAA‑CCCCGCCAGTAGCGTGGGTGGAGGaa                                                                                                                 <  1:593313/141‑1 (MQ=255)
                                      cccATTGCCCGCCCCAGCCCCGCCACCCGGCCAGAGATTTGGGAAGGGGTGGCCCCGGCGACAACCGCTCCTGCCAAAATTATTGCCGCGAAACGGGCG‑TAGACAATTAATCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAAt                                                                                                                <  2:395430/149‑1 (MQ=255)
                                      cccATTGCCAGCCCCAGCGCCCTCTCCCGGCAAGAGTTTTGGCCTGTGGGGGCTCCGGCTAAATCCGCTGCTTGCCAGTTTATTGCCGGTGACCTGGTG‑GATCAAATTAATGCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGaa                                                                                                                 <  2:283075/148‑1 (MQ=255)
                                      ccaattcccgcccccGGCGCCGTCACCCAGCAAGAGTTTTGTGCGGGGGGGGCTCGGGCGAAATTCGCTGCTTGCAAGTTTTTTGCCGTTGAACGGGGG‑TACCAAATAAACCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAg                                                                                                              <  4:487596/140‑1 (MQ=255)
                                        cATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGa                                                                                                             >  1:453443/1‑150 (MQ=255)
                                        cATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGa                                                                                                             >  1:1234318/1‑150 (MQ=255)
                                          ttGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGaaa                                                                                                           >  4:1504461/1‑150 (MQ=255)
                                          tggccgcccccAGCCCCGCCACCCGGCGAGGGTTTTGTGCAGTGGGGGCGCCGGCAAAACTCGCTGTTTGCAAAATTATGCCCGCTGACGTGGGG‑GAGCAAATAAATCCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGaaa                                                                                                           <  3:227810/143‑1 (MQ=255)
                                           tGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAAc                                                                                                         >  4:1210302/1‑151 (MQ=255)
                                           tGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAAc                                                                                                         >  1:1535389/1‑151 (MQ=255)
                                             ccACCCCCAGCGCCGTCATCCAGCCAGAGTTTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCAAGATTATGGCCGCTGAGCTGGCG‑TAGCAAATTAATCCCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGa                                                                                                             <  4:215621/145‑1 (MQ=255)
                                               aGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc                                                                                                     >  1:1757427/1‑151 (MQ=255)
                                                 ccccAGCGCCGCCACCCACCCAGAGATTTGGGCAGGGGGGCCCCCGCCAAAAATCGCTCCTTCCAAAATTTTTGCCGGTAACCGGGCT‑TA‑CCAAATAAACCCCCCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc                                                                                                     <  4:1373429/149‑1 (MQ=255)
                                                  cccAGCGCCATCATCCACCCAAAGGTTGGGGCGGGGGTGCCTCGGGCGAAAATCGCTGTGTCCAATATTATGCCCCGAGACCTGGCG‑TACCAAATAAATACCGCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCa                                                                                                  <  1:332311/151‑1 (MQ=255)
                                                     aGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCAt                                                                                                 >  1:818900/1‑149 (MQ=255)
                                                      gcgcCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGt                                                                                              >  4:1729924/1‑151 (MQ=255)
                                                       cgcCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTc                                                                                             >  4:1400889/1‑151 (MQ=255)
                                                       ccccGTCACCCAGCAAGAGTTTTGTGAAGTGGTGCCCCCGGCAAAAAGCGTTGCTGGCAAGATTATTGCGGCTGACCGGGAG‑AAGCAAATAAAACCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGt                                                                                              <  1:1910422/148‑1 (MQ=255)
                                                        gcCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTc                                                                                             >  1:1749105/1‑150 (MQ=255)
                                                        gcCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTc                                                                                             >  1:1787816/1‑150 (MQ=255)
                                                         ccGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCAc                                                                                           >  4:1059868/1‑151 (MQ=255)
                                                           gTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCt                                                                                         >  3:312874/1‑151 (MQ=255)
                                                             cacccaccaagatATTGGTGCAGGGGTGGCTCGGGGAACAACCGCTGTTGGCCAGTTTATGGCCGCGAAAGGGGGG‑TAACAAATTAACCCCGCAAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAg                                                                                               <  1:594415/134‑1 (MQ=255)
                                                              acccagccagAGATTTGTGCGGGGGTGGCTCCGGCGACAATCGCTGTTGGCCAGATTATTCCCGCTGAACGGGCT‑TAGCAAATTAATCCCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGt                                                                                      <  2:88348/149‑1 (MQ=255)
                                                                    ccagAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGtt                                                                                 >  4:526534/1‑150 (MQ=255)
                                                                    caagagATTGGTGCGGGGGTGGCCCCGGCAACAATCGCTGCTTGCCAGTTTTTGCCCGTTGACCGGGCG‑TAGCAAATTAATCCCGCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTta                                                                                <  1:990455/149‑1 (MQ=255)
                                                                         gttttGTGCAGTGGGGGCCCCGGCGACAACCGCTTCTTCCCAGATTATTCCCGCTAATCTGGCG‑TGGCAAATAAATACCACCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGc                                                                            <  4:41610/148‑1 (MQ=255)
                                                                          aTTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGt                                                                          >  4:403833/1‑151 (MQ=255)
                                                                           tttGTGCGGGGGTGGCCCGGGCGACAATCGCTGCTGGCCAGATTATGGCCGTGGACCGGGGG‑TAGCAAATTAACCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtat                                                                               <  3:432631/145‑1 (MQ=255)
                                                                           tttGTGCGGGGGTGGCCCGGGCAAAAATCGCTGCTTCCAAGTTTATGCCCGCTAAACGGGCG‑TAGCCAATAAATCCCCCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTta                                                                                <  4:1001213/144‑1 (MQ=255)
                                                                            tgttgCAGTGGTGGCTCGGGGTACAACCGCTGCTGTCAATAGTATCGCC‑TTTAACTGGGGTTAGAAAATAAACACCCCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGt                                                                          <  3:129761/146‑1 (MQ=255)
                                                                                 cgggggggccTCCGGCACCATCCGCGGCTGCCCAGATTATTGCCGTAGACGGGGGG‑AAGCAAATTAACCCCGCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGc                                                                    <  1:927786/141‑1 (MQ=255)
                                                                                 cAGTGGTGGCTCGGGCGACAATCCCTGCTTGCCAGATTGTTCCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTTGGTGGCGGAATAGAAAACACACGCATAGTCACCTCGTGCGTTATAGCGCACAGGc                                                                    >  3:418513/1‑150 (MQ=255)
                                                                                 cAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGc                                                                    >  3:263107/1‑150 (MQ=255)
                                                                                  aGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAc                                                                  >  4:1711258/1‑151 (MQ=255)
                                                                                    tggtggCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCg                                                                >  3:258527/1‑151 (MQ=255)
                                                                                    tggtggCTCCGGCAACAATCGCTTTTTGCCAGATTATGGCCGCTGAACTGGCG‑TACCAAATAAACCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAcc                                                                 <  4:736996/150‑1 (MQ=255)
                                                                                    gggtgcCTCCGGCAAAAATCCCTGTTCGCAAGTTTATTGCCGTGGACGGGGCG‑TAGCAAATAAACCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCa                                                                   <  1:1039164/147‑1 (MQ=255)
                                                                                     ggtggCCCCGGCAAAATTCGCTGCTTGCAAAATTTTGGCCCCTAAATGGGCG‑TACCAAATAAATCCCGCAAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGt                                                               <  4:1697354/151‑1 (MQ=255)
                                                                                      gtggCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACGGGCG‑TAGCAAATAAATCCCCCAAAAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTa                                                              <  4:1756564/151‑1 (MQ=255)
                                                                                        gcccccgccaacacTCGCTGCTTGCCAGATTATTGCCGCTGAGCTGGAA‑GAGCCAAAAAATCCCGCCAAAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAg                                                             <  2:2629/140‑1 (MQ=255)
                                                                                            cgggCAACAACCGCTGTTTGCCAGATTATGGCCGCTGACCGGGGG‑TAGCAAATTAATCCCCCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCAttt                                                        <  4:1625430/149‑1 (MQ=255)
                                                                                            ccGGCAACAACCGCTGCTTGCCAGATTATTGCCGCTGACCGGGGG‑TAGCAAATTAATACCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCAttt                                                        <  1:713993/151‑1 (MQ=255)
                                                                                                  aCAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGt                                                  >  4:1632650/1‑151 (MQ=255)
                                                                                                    aaccGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTta                                                <  4:301238/148‑1 (MQ=255)
                                                                                                    aaccGCTGCTTGCCAAATTTTGCCCGCTGACCTGGCG‑TAGCAAATTAATCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGt                                                  <  4:1012710/146‑1 (MQ=255)
                                                                                                     aTCGCTGTTTCCCAGATTATTGCCGCTGAACGGGCG‑TACCCAATAAATCCCCCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTtat                                               <  4:1487013/151‑1 (MQ=255)
                                                                                                      tCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTtat                                               >  1:571513/1‑150 (MQ=255)
                                                                                                       cGCTGCTTGCAAGTTTATTGCCGCTAAACGGGCG‑TAGCCAATTAATCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTtat                                               <  4:1798578/149‑1 (MQ=255)
                                                                                                        gctgctTGCCATTTTATGGCCGGTAACCGGGGG‑TACCAAATAAATCCCGCCAAAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATaa                                             <  1:766713/150‑1 (MQ=255)
                                                                                                        gctgctTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAAc                                            >  4:1017117/1‑151 (MQ=255)
                                                                                                        gctgctTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGAGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAAc                                            >  3:186655/1‑151 (MQ=255)
                                                                                                          tgctTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGc                                          >  4:1519955/1‑151 (MQ=255)
                                                                                                           gctTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGc                                          >  1:407315/1‑150 (MQ=255)
                                                                                                             ttGCCAAATTATGCCCGCTGAAGTGGGG‑TAGCAAATTAACCCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTtata                                              <  1:1125753/144‑1 (MQ=255)
                                                                                                              tcccAAATTTTTGCCGCGGAACGGGGG‑TAGCAAATAAATCCCCCAAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGcaca                                       <  1:1500294/148‑1 (MQ=255)
                                                                                                              tGCCAGATTTTGGCCGTGGAACGGGGA‑TAGCAAATAAAACCCGCCAAAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCg                                                   <  1:1172544/138‑1 (MQ=255)
                                                                                                                caagatattggCCGCTGAACTGGTG‑GACAAAATTAACACCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAg                                     <  4:591304/140‑1 (MQ=255)
                                                                                                                     tttttGCCGTTGAACGGGCG‑TACCAAATAAATCCCCCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTg                                   <  1:344197/144‑1 (MQ=255)
                                                                                                                     ttattGCCGCTGAACGGGCG‑TAGCAAATTAATCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGa                                  <  4:1694667/148‑1 (MQ=255)
                                                                                                                     ttattGCCGCTAAACTGGCG‑TAGCAATTTAATCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGAt                                 <  2:386655/149‑1 (MQ=255)
                                                                                                                       atggCCGCGGAACTGGCG‑TAGCAAATTAATCCCGCAAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATaaa                              <  2:313861/147‑1 (MQ=255)
                                                                                                                          gCCGCTGAACGGGCG‑TAGCAAATTAATCCCGCAAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACt                            <  4:1058946/149‑1 (MQ=255)
                                                                                                                             gtgaacgggggg‑TAGCAAATTAATCCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGt                       <  1:1698461/140‑1 (MQ=255)
                                                                                                                             gCTGAATTGCGG‑TAGCTATTAAAGCCCCCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGt                       <  4:557809/151‑1 (MQ=255)
                                                                                                                                 accgggCG‑TAGCAAATTAATCCCCCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGtttt                    <  4:1205722/146‑1 (MQ=255)
                                                                                                                                 accgggCG‑TAGCAAATTAACCCCGCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTg                   <  1:1209622/147‑1 (MQ=255)
                                                                                                                                 aaCTGGGG‑TGGCCATTTAATCCCGCCAAAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCg                        <  4:1793989/146‑1 (MQ=255)
                                                                                                                                 aaCGGGCG‑TAGCAAATAAATCCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTg                   <  4:1616063/151‑1 (MQ=255)
                                                                                                                                 aaCGGGCG‑TAGCAAATAAACCCCCCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGtttt                    <  1:315638/150‑1 (MQ=255)
                                                                                                                                  acgggCG‑TAGCAAATTAATCCCGCAAAAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGttt                     <  4:22654/145‑1 (MQ=255)
                                                                                                                                   cTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGcc                 >  1:1313853/1‑151 (MQ=255)
                                                                                                                                     ggCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCgg               >  1:959974/1‑151 (MQ=255)
                                                                                                                                         gtaccaaaTAAATCCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggag             <  4:1307758/144‑1 (MQ=255)
                                                                                                                                           aGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggagg            >  4:1788678/1‑149 (MQ=255)
                                                                                                                                              aaaTAAATCCCCCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggagga           <  1:912830/146‑1 (MQ=255)
                                                                                                                                                aTTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGc      >  4:774698/1‑150 (MQ=255)
                                                                                                                                                 taaaTCCCGCCAGAGT‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTc        <  3:512644/145‑1 (MQ=255)
                                                                                                                                                   aaTACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATgg  >  4:135663/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
GTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCG‑TAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGG  >  NC_000913/1505879‑1506175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑