Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC40.9% 240.4 / 34.8 128intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (66/9);  new base C (0/52);  total (66/62)
Fisher's exact test for biased strand distribution p-value = 1.15e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

CGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001771‑2002066
                                                                                                                                                      |                                                                                                                                                     
  gTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCccg                                                                                                                                                 >  1:1215859/1‑151 (MQ=255)
    ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCccgc                                                                                                                                                <  1:275231/150‑1 (MQ=255)
          tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTACCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGc                                                                                                                                         >  4:1360976/1‑151 (MQ=255)
            aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCgg                                                                                                                                       >  4:350599/1‑151 (MQ=255)
             aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCggg                                                                                                                                      >  1:1552644/1‑151 (MQ=255)
                      aaaCAGCCCTGCTTTATATGAGTTATCGGCATGATTATCCGTTTCTGCGGGTTTTTTAACCGGACGATAGGGGGG‑GGAAATGAGGGGTTATTG‑GGGGGTTAAAGGA‑AAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCgggg                                                                                                                                     <  4:436858/143‑1 (MQ=255)
                      aaaCAGCCCTGCGTTATATGAGTTACCGGCAGGATTTTCCTTTTCTGCGGGTTTTTTAACCGGACGATTAGTGGG‑GAAAAGGGGGGTTTATTG‑GGGGGTTACAGTT‑AAATCCCGGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAg                                                                                                                                  <  4:1198475/146‑1 (MQ=255)
                       aaCGGCCCTGGTTTATATGATTTATGGGCTTGATTATCCGTTTCTGCGGGTTTTTTAATCGGAGGTTTGGGGGG‑TGAAAGGGGGGGTTATTT‑GGGGGTTACGGGT‑AAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                            <  1:1732396/151‑1 (MQ=255)
                       aaCAGCCCGGGGTTATAGGAGTTACCGGAATGTTTATCCTTTTCTGCGGGGTTTTAAATGGGAGGATTAGGGGG‑GAAAATGAGGGTTTATTG‑GGGGGTTACAGTA‑AATTTCCGGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                            <  4:1292864/151‑1 (MQ=255)
                            cccTGCTTTATATGAGTTATCGGCATGATTATCCGTTTCTGCGGTTTTTTTAAGGGGAGGATTGGTGGG‑TGAAATGAGGGGTT‑TTT‑GGGGGTTAAAAGTAAAATTCCAGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGa                                                                                                                       <  1:1099467/151‑1 (MQ=255)
                            cccGGCTTTTTATGGTTTATCGGCATGTTTATCCTTTTCTGCGGGGTTTTAAACCGGACG‑TTAATGGGATAAAATAGGGGTTTATTG‑GGGGTTTACA‑GT‑AAAATTCAGGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGa                                                                                                                       <  4:546172/151‑1 (MQ=255)
                                gCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaa                                                                                                                    >  4:1404028/1‑150 (MQ=255)
                                  gTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                 >  4:60324/1‑151 (MQ=255)
                                   ttATATGATTTATGGGCATGATTATCCGTTTCTGCGGGGTTTTTAATCGGATTATTAGTGGG‑TGAAATGAGGGGTTATTG‑GGGGGTTACAGGT‑AAATTCCAGAAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                                  <  4:297759/149‑1 (MQ=255)
                                   ttATATGATTTATCGGCATTATTGTCCGTTTCTGCAGGGTTTTTAAGCGGACGATTGGGGGG‑TGAAAGGGGGGTTTTTTT‑GGGGGTTAAAGGA‑AATTCCCAGCAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa                                                                                                                     <  3:529088/146‑1 (MQ=255)
                                   ttATATATGTTATGGTCATGTTTACCCGTTTCTGCAGGTTTTTTAACCGGACGATTTGTGGG‑TAAAAGGGGGGTTTTTTT‑GGGGTTTAGAGGT‑AAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                                  <  1:838486/149‑1 (MQ=255)
                                   ttATAGGATTTATCGGCATGTTTATCCTTTTCTGCAGGTTTTTTAATCGGACGATTGGGGGG‑GGAAAGGGGGGTTTTTTG‑GGGGGTTACAGGT‑AATTCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                                   <  1:38967/148‑1 (MQ=255)
                                       atGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCt                                                                                                            >  2:40818/1‑151 (MQ=255)
                                       atGAGTTATCGGCATGATTATCCGTTTCTGCAGGGGTTTTAATCGGACGATTAGCGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGGAGCACGTTGCTGACAAATTGCGCt                                                                                                            >  1:19404/1‑151 (MQ=255)
                                        tGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCtt                                                                                                           >  4:1691683/1‑151 (MQ=255)
                                         gAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                          >  4:1035972/1‑151 (MQ=255)
                                            ttATGGGCATGATTATCCGTTTCTGCAGGTTTTTTAACCGGACGTTTAGTGGG‑AAAAAGGGGGGTTTATTT‑GGGGTTTACAGTT‑AATTCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                                <  1:1019448/142‑1 (MQ=255)
                                            ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                        >  1:746454/1‑150 (MQ=255)
                                             tATCGGCATGATTATCCGTTTCTGCAGGGGTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                      >  1:1061847/1‑151 (MQ=255)
                                              aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGTTTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGGTACAGGT‑AAATTCCAAGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCCGACAAATTGCGCtttttttt                                                                                                     >  2:493767/1‑146 (MQ=255)
                                              aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGtt                                                                                                     >  2:348584/1‑151 (MQ=255)
                                               tCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTg                                                                                                    >  3:9959/1‑151 (MQ=255)
                                                       aTTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                            >  3:465301/1‑151 (MQ=255)
                                                       aTTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                            >  4:720524/1‑151 (MQ=255)
                                                        ttATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                           >  4:1867962/1‑151 (MQ=255)
                                                        ttATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                           >  1:1402010/1‑151 (MQ=255)
                                                        ttACCCTTTTCTCCAGGTTTTTTATCCGGACTATTGGTGGG‑GAAAAGGAGGGTTTTTTG‑GGGGTTTACAGG‑‑AAATTCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                            <  4:407437/150‑1 (MQ=255)
                                                            cggTTTCGGCGGGGTTTTAAATCGGAGGTTAGGGGGG‑TAAAATGGGGGTTTTTTG‑GGGGGTTACGGTT‑AAATTCAAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       <  3:32241/149‑1 (MQ=255)
                                                             cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       >  4:842846/1‑150 (MQ=255)
                                                             cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       >  1:241758/1‑150 (MQ=255)
                                                              gTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                                      >  4:1426336/1‑150 (MQ=255)
                                                               tttCTGCGGGGTTTTTAAGCGGACGATTGGGGGG‑GAAAAGGAGGGTTTATTG‑GGGGGTTACAGGT‑AAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                        <  3:333046/147‑1 (MQ=255)
                                                                ttCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                                   >  2:71977/1‑151 (MQ=255)
                                                                 tCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                                   >  4:1730006/1‑150 (MQ=255)
                                                                    gCAGGTTTTTTAATGGGACGATTAGGGGG‑GAAAAGGGGGGTTTATTG‑GGGGGTTACAGGA‑AAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                               <  4:1830425/151‑1 (MQ=255)
                                                                    gCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                               >  3:518170/1‑151 (MQ=255)
                                                                      aGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                              >  3:421371/1‑150 (MQ=255)
                                                                      aGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                              >  3:195838/1‑150 (MQ=255)
                                                                       gggTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                            >  1:1325611/1‑151 (MQ=255)
                                                                       gggTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGGTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGACGGACGCGGCGTCAACGCCTTAt                                                                            >  1:1177610/1‑151 (MQ=255)
                                                                       gggTTTTTAAGCGGACGATTAGTGGG‑TGAAAGGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                              <  4:560706/149‑1 (MQ=255)
                                                                        ggTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                           >  2:60306/1‑151 (MQ=255)
                                                                            tttAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                       >  4:1489040/1‑151 (MQ=255)
                                                                               actCGGACGATTGGGGGG‑TGAAAGGGGGGGTTTTTG‑GGGGTTTAGGGGT‑AAATTCCGGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     <  4:1376925/148‑1 (MQ=37)
                                                                               aaggggACGATTAGGGGG‑TGAAAGGAGGGGTTATTG‑GGGGGTTACAGGT‑AAATTCCAGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                       <  3:472396/144‑1 (MQ=39)
                                                                               aaccGGACGATTAGTGGG‑TAAAATGGGGGTTTATTT‑GGGGGTTACAGGA‑AAATCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     <  4:240538/147‑1 (MQ=38)
                                                                               aaTCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  1:1624180/1‑150 (MQ=255)
                                                                                 tCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   >  4:1509578/1‑150 (MQ=255)
                                                                                   ggACGTTTGGTGGG‑TAAAATGAGGGGTTATTG‑GGGGTTTACAGGA‑AAATTCCAGGCAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                                <  2:182419/151‑1 (MQ=38)
                                                                                   ggACAATTGGTGGG‑TAAAAGGTGGGTTTGTTG‑GGGGTTTAAGGGA‑AAATCCCAGGCAGAAAAAAT‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                                <  1:466917/151‑1 (MQ=25)
                                                                                   gaaggATTAGGGGG‑TGAAATGGGGGTTTTTTG‑GGGGGTTACAGGT‑AAATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                 <  1:566531/146‑1 (MQ=38)
                                                                                    gACGATTGGTGGG‑TAAAATGAGGGGTTATTG‑GGGGGTTACAGGT‑AAATCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                               <  1:1466952/151‑1 (MQ=38)
                                                                                     aCGATTGGTGGG‑TAAAAGGGGGGGTTTTTG‑GGGGGTTACAGGA‑AAATCCCAGGAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                                              <  1:1219179/151‑1 (MQ=37)
                                                                                         ttgggggg‑TAAAAGGGGGGGTTTTTG‑GGGGGTTACAGG‑‑AAATTCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                           <  1:681600/145‑1 (MQ=25)
                                                                                          tAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                         >  1:312065/1‑151 (MQ=255)
                                                                                            ggggg‑TAAAAGGGGGGTTTATTT‑GGGGGTTACGGGT‑AAATCCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                           <  4:1595409/145‑1 (MQ=37)
                                                                                            gTGGG‑TGAAATGGGGGTTTTTTT‑GGGGTTTACAGGT‑AAATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                                        <  4:1724616/150‑1 (MQ=38)
                                                                                            gTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                                        >  1:1868966/1‑150 (MQ=255)
                                                                                             tGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                                       >  1:652239/1‑150 (MQ=255)
                                                                                               gg‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                                    >  4:804109/1‑151 (MQ=255)
                                                                                                    aaaggggggtTTTTTT‑GGGGGTTAAAGAA‑AATTCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                                <  1:301836/145‑1 (MQ=25)
                                                                                                    aaaTGAGGGTTTTTTT‑GGGGTTTACAGAT‑AAATTCCAGAAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                                  <  1:676609/149‑1 (MQ=37)
                                                                                                    aaaTGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                                >  2:532483/1‑151 (MQ=255)
                                                                                                    aaaGGAGGGTTTATTG‑GGGGGTTACAGGT‑AAATTCCGGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa                                                 <  4:637523/150‑1 (MQ=37)
                                                                                                     aaTGGGGGGTTATTT‑GGGGGTTACAGGT‑AATTTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                               <  2:439350/151‑1 (MQ=255)
                                                                                                      aTGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                               >  4:1320787/1‑150 (MQ=255)
                                                                                                       tGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                              >  4:1876588/1‑150 (MQ=255)
                                                                                                         gggggTTTTTTGGGGGGTTACAGGT‑AAATCCCGGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                              <  4:568466/148‑1 (MQ=25)
                                                                                                          ggggTTATGG‑GGGGGTTAAAGAT‑AAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaaa                                             <  1:172248/148‑1 (MQ=37)
                                                                                                                  ttggggggTTAAAGGT‑AAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     <  1:1503691/146‑1 (MQ=255)
                                                                                                                   t‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAAc                                 >  2:135052/1‑151 (MQ=255)
                                                                                                                     ggggtTTACAGAT‑AAATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCaa                                  <  1:745883/149‑1 (MQ=255)
                                                                                                                     gggggTTACAGGT‑AAATTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACt                                <  4:1532541/151‑1 (MQ=255)
                                                                                                                      ggggTAAAAGGA‑AATTCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACtt                               <  1:1377533/151‑1 (MQ=25)
                                                                                                                       ggtttACAGGT‑AAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                              <  1:842484/148‑1 (MQ=255)
                                                                                                                       ggtttACAGGA‑AATTTCAAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                              <  1:1032554/148‑1 (MQ=37)
                                                                                                                           taagggT‑AAATTCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                           <  1:1836808/146‑1 (MQ=255)
                                                                                                                            aCAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                          >  1:1529722/1‑150 (MQ=255)
                                                                                                                             cAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                        >  1:249444/1‑151 (MQ=255)
                                                                                                                               ggT‑AAATCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt                       <  2:422761/150‑1 (MQ=255)
                                                                                                                                   aaattcaggaaaGAAAAAAC‑CGCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCGGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                   <  3:372507/140‑1 (MQ=25)
                                                                                                                                   aaaTTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                    >  1:57845/1‑150 (MQ=255)
                                                                                                                                   aaaTTCCAGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg                      <  1:507136/148‑1 (MQ=255)
                                                                                                                                    aaTTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGCAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                   >  1:1888522/1‑150 (MQ=255)
                                                                                                                                    aaTTCCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATaa                  <  3:222347/151‑1 (MQ=255)
                                                                                                                                      ttCCAGGCAGAAAAAAA‑CCCCGCCGGGGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagc                >  1:427859/1‑151 (MQ=255)
                                                                                                                                        ccAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc              >  4:230359/1‑151 (MQ=255)
                                                                                                                                        ccAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc              >  2:95737/1‑151 (MQ=255)
                                                                                                                                        ccAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcg               <  2:481123/150‑1 (MQ=255)
                                                                                                                                         cAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc              >  1:1481915/1‑150 (MQ=255)
                                                                                                                                           ggCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagc                <  3:179706/146‑1 (MQ=255)
                                                                                                                                             cAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcgc         >  4:594466/1‑151 (MQ=255)
                                                                                                                                              aaaaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcg          <  1:711745/147‑1 (MQ=255)
                                                                                                                                              aGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcgc         >  1:1794164/1‑150 (MQ=255)
                                                                                                                                                    aaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc  >  1:829453/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGG‑TGAAATGAGGGGTTATTT‑GGGGGTTACAGGT‑AAATTCCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001771‑2002066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑