BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	34,876	A→G	C166R (TGT→CGT)	caiE ←	stimulator of CaiD and CaiB enzyme activities

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	34,876	0	A	G	100.0%	488.2 / NA	135	C166R (TGT→CGT)	caiE	stimulator of CaiD and CaiB enzyme activities
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (68/67); total (68/67)

Only 100 of 135 total aligned reads displayed.

CCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTGACAAAGCT  >  NC_000913/34728‑35017

    aTGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGccc                                                                                                                                         <  4:561180/151‑1 (MQ=255)

    aTGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGccc                                                                                                                                         >  2:266060/1‑151 (MQ=255)

       cGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGAc                                                                                                                                      >  1:242116/1‑151 (MQ=255)

        gATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACa                                                                                                                                     <  4:647582/151‑1 (MQ=255)

           gttgtAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGa                                                                                                                                  <  4:876486/151‑1 (MQ=255)

             tgtAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGAt                                                                                                                                 <  4:536215/150‑1 (MQ=255)

                aGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCt                                                                                                                              <  1:628872/150‑1 (MQ=255)

                aGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCt                                                                                                                              <  4:942130/150‑1 (MQ=255)

                aGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCt                                                                                                                              <  3:158362/150‑1 (MQ=255)

                aGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCt                                                                                                                              <  1:1657425/150‑1 (MQ=255)

                    tCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATAc                                                                                                                         >  2:488875/1‑151 (MQ=255)

                       tATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATa                                                                                                                          <  4:459423/147‑1 (MQ=255)

                        aTCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCtt                                                                                                                     <  4:1710606/151‑1 (MQ=255)

                         tCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCttt                                                                                                                    >  1:1584250/1‑151 (MQ=255)

                          ccAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCttt                                                                                                                    <  3:266052/150‑1 (MQ=255)

                          ccAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCttt                                                                                                                    <  1:1425051/150‑1 (MQ=255)

                             gCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGt                                                                                                                 >  4:605004/1‑150 (MQ=255)

                             gCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTa                                                                                                                <  1:78302/151‑1 (MQ=255)

                               aaCCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTAtt                                                                                                              >  4:984330/1‑151 (MQ=255)

                                 ccAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTc                                                                                                             >  1:1590355/1‑150 (MQ=255)

                                 ccAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCa                                                                                                            <  1:627321/151‑1 (MQ=255)

                                  cAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCaa                                                                                                           <  4:1628653/151‑1 (MQ=255)

                                  cAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCa                                                                                                            >  3:535360/1‑150 (MQ=255)

                                    ggTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAAcc                                                                                                         >  1:12485/1‑151 (MQ=255)

                                       cGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGtt                                                                                                      >  2:478725/1‑151 (MQ=255)

                                        gCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGttt                                                                                                     >  1:1450448/1‑151 (MQ=255)

                                         cATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTc                                                                                                    <  4:915146/151‑1 (MQ=255)

                                           tCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTcc                                                                                                   >  1:1364407/1‑150 (MQ=255)

                                            ccGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCa                                                                                                  <  2:521223/150‑1 (MQ=255)

                                            ccGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAg                                                                                                 <  4:484478/151‑1 (MQ=255)

                                              ggCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAgtg                                                                                               <  4:1426840/151‑1 (MQ=255)

                                              ggCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAgtg                                                                                               <  4:832300/151‑1 (MQ=255)

                                              ggCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAgtg                                                                                               >  4:856339/1‑151 (MQ=255)

                                              ggCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAgt                                                                                                >  4:1357404/1‑150 (MQ=255)

                                                cAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTa                                                                                             <  4:28477/151‑1 (MQ=255)

                                                 aaGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTa                                                                                             >  1:621629/1‑150 (MQ=255)

                                                  aGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAAc                                                                                           <  3:22232/151‑1 (MQ=255)

                                                   gATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACt                                                                                          <  1:358216/151‑1 (MQ=255)

                                                    aTCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACtc                                                                                         >  4:735485/1‑151 (MQ=255)

                                                      cACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACtcgt                                                                                       >  1:519928/1‑151 (MQ=255)

                                                      cACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACtcgt                                                                                       >  4:1905919/1‑151 (MQ=255)

                                                       aCCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACtcgtc                                                                                      <  4:595340/151‑1 (MQ=255)

                                                       aCCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACtcgtc                                                                                      >  3:186202/1‑151 (MQ=255)

                                                        ccGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACtcgtc                                                                                      <  4:7036/150‑1 (MQ=255)

                                                         cGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTcgtcgt                                                                                    >  4:259106/1‑151 (MQ=255)

                                                         cGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTcgtcgt                                                                                    >  1:1002360/1‑151 (MQ=255)

                                                         cGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTcgtcgt                                                                                    >  1:405674/1‑151 (MQ=255)

                                                           tttAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCgtcgtc                                                                                   >  3:419211/1‑150 (MQ=255)

                                                            ttAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCAc                                                                                 >  1:732002/1‑151 (MQ=255)

                                                                gCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAAc                                                                             <  4:607484/151‑1 (MQ=255)

                                                                    cacaTCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACAtt                                                                          <  4:1082524/150‑1 (MQ=255)

                                                                       aTCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGt                                                                      <  1:221467/151‑1 (MQ=255)

                                                                         ccGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTAc                                                                    <  1:989872/151‑1 (MQ=255)

                                                                          cgtcgtCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACa                                                                   <  4:1760570/151‑1 (MQ=255)

                                                                           gtcgtcCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAg                                                                  <  4:115588/151‑1 (MQ=255)

                                                                            tcgtcCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAgc                                                                 >  1:1054477/1‑151 (MQ=255)

                                                                             cgtcCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAgc                                                                 <  4:644610/150‑1 (MQ=255)

                                                                                    tGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTg                                                         >  3:70737/1‑151 (MQ=255)

                                                                                    tGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTg                                                         >  4:786598/1‑151 (MQ=255)

                                                                                     gCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCt                                                          <  1:1735035/149‑1 (MQ=255)

                                                                                     gCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCt                                                          <  1:1036329/149‑1 (MQ=255)

                                                                                      cAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGc                                                       <  2:149167/151‑1 (MQ=255)

                                                                                      cAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGAAGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTgg                                                        >  4:222544/1‑150 (MQ=255)

                                                                                       aaaCGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCg                                                      >  1:5274/1‑151 (MQ=255)

                                                                                        aaCGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCg                                                      >  4:757063/1‑150 (MQ=255)

                                                                                        aaCGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGt                                                     >  4:1853648/1‑151 (MQ=255)

                                                                                            ggggCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTAccc                                                 <  2:134886/151‑1 (MQ=255)

                                                                                             gggCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCa                                                <  3:102010/151‑1 (MQ=255)

                                                                                              ggCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCAt                                               <  1:592686/151‑1 (MQ=255)

                                                                                               gCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATc                                              >  4:855027/1‑151 (MQ=255)

                                                                                               gCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATc                                              >  4:1264298/1‑151 (MQ=255)

                                                                                                 gATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATca                                             >  3:469179/1‑150 (MQ=255)

                                                                                                    tttCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATcaaca                                          <  2:266504/150‑1 (MQ=255)

                                                                                                       cctccATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTg                                      >  4:1561298/1‑151 (MQ=255)

                                                                                                         tccATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGc                                    >  4:1413391/1‑151 (MQ=255)

                                                                                                          ccATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCg                                   >  1:1286775/1‑151 (MQ=255)

                                                                                                            aTTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGtt                                 <  1:510983/151‑1 (MQ=255)

                                                                                                            aTTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGtt                                 >  2:139336/1‑151 (MQ=255)

                                                                                                              ttGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGttt                                <  4:544503/150‑1 (MQ=255)

                                                                                                               tGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTtct                              >  1:711720/1‑151 (MQ=255)

                                                                                                                   tCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTcgcc                          >  1:255811/1‑151 (MQ=255)

                                                                                                                   tCAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTcgcc                          >  2:523512/1‑151 (MQ=255)

                                                                                                                    cAGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTcgcc                          <  3:498991/150‑1 (MQ=255)

                                                                                                                     aGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTcgccgc                        <  4:1303502/151‑1 (MQ=255)

                                                                                                                     aGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTcgccgc                        <  4:1408035/151‑1 (MQ=255)

                                                                                                                     aGTGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAACGCGGGCTGGCGTACCCATCAACAAATGGCGTTTCTcgccgc                        >  1:539340/1‑151 (MQ=255)

                                                                                                                       tGGCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCcgcg                       >  1:1865228/1‑150 (MQ=255)

                                                                                                                        ggCTGCGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGaa                     <  4:110425/151‑1 (MQ=255)

                                                                                                                             cGTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGccc                <  4:412488/151‑1 (MQ=255)

                                                                                                                              gTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCg               <  1:1252926/151‑1 (MQ=255)

                                                                                                                              gTTTCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCg               >  4:1539129/1‑151 (MQ=255)

                                                                                                                               tttCATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGc              >  4:1246109/1‑151 (MQ=255)

                                                                                                                                  cATGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCttt           <  4:1097676/151‑1 (MQ=255)

                                                                                                                                    tGTAACGATACATGACGGCGCCCGACAAGATCCTGCTACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTg          >  4:273508/1‑150 (MQ=255)

                                                                                                                                    tGTAACGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTg          >  4:243087/1‑150 (MQ=255)

                                                                                                                                       aaCGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTGACa       <  4:87029/150‑1 (MQ=255)

                                                                                                                                       aaCGATACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTGACa       >  1:59851/1‑150 (MQ=255)

                                                                                                                                        aCGATACATGACGGCGCACGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTGACaaa     <  4:1807233/151‑1 (MQ=255)

                                                                                                                                           aTACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTGACAAAGCt  >  4:307035/1‑151 (MQ=255)

                                                                                                                                           aTACATGACGGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTGACAAAGCt  <  1:1840623/151‑1 (MQ=255)

CCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTCGTCACTAACATTGCGTACAGCGCGGGCTGGCGTACCCATCAACAACTGGCGTTTCTCGCCGCGAAAGCCCGCTTTGACAAAGCT  >  NC_000913/34728‑35017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`