BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,442,524	C→T	A254V (GCG→GTG)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,442,524	0	C	T	100.0%	643.1 / NA	161	A254V (GCG→GTG)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (81/80); total (81/80)

Only 100 of 163 total aligned reads displayed.

ACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGATGA‑GGCCCCCGCACTGGGTG  >  NC_000913/2442379‑2442670

aCGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGt                                                                                                                                                  >  3:431866/1‑151 (MQ=255)

  gCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTgg                                                                                                                                                <  4:472679/151‑1 (MQ=255)

  gCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTgg                                                                                                                                                >  1:1431283/1‑151 (MQ=255)

   cTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGt                                                                                                                                               >  3:365020/1‑151 (MQ=255)

        gACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAAc                                                                                                                                          >  1:22696/1‑151 (MQ=255)

         aCATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAAcc                                                                                                                                         >  1:1233168/1‑151 (MQ=255)

         aCATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAAcc                                                                                                                                         >  4:609086/1‑151 (MQ=255)

          cATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCg                                                                                                                                        >  1:1514823/1‑151 (MQ=255)

           aTTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGc                                                                                                                                       >  1:482730/1‑151 (MQ=255)

            tttACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGc                                                                                                                                       >  1:852955/1‑150 (MQ=255)

             ttACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCCCCGTGCCGTGGTTTAACCGCa                                                                                                                                      <  3:85491/150‑1 (MQ=255)

             ttACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCACCTGCTCCGTGCCGTGGTTTAACCGCa                                                                                                                                      <  4:460869/150‑1 (MQ=255)

              tACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCAc                                                                                                                                     <  1:924840/150‑1 (MQ=255)

               aCGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACg                                                                                                                                    <  1:1136387/150‑1 (MQ=255)

                cGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACggg                                                                                                                                  <  4:1363976/151‑1 (MQ=255)

                     gCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGgcagca                                                                                                                             <  4:1730661/151‑1 (MQ=255)

                     gCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGgca                                                                                                                                <  1:202187/148‑1 (MQ=255)

                        ggTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGgcagcagc                                                                                                                           >  4:558347/1‑150 (MQ=255)

                         gTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGgcagcagca                                                                                                                          >  2:127700/1‑150 (MQ=255)

                         gTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGgcagcagca                                                                                                                          >  1:1203057/1‑150 (MQ=255)

                          ttttGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCaa                                                                                                                         >  4:105084/1‑150 (MQ=255)

                            ttGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACg                                                                                                                      >  1:503701/1‑151 (MQ=255)

                             tGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACg                                                                                                                      <  2:206122/150‑1 (MQ=255)

                               tCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACggg                                                                                                                    >  4:1243672/1‑150 (MQ=255)

                                  gccgcGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAgc                                                                                                                <  1:649866/151‑1 (MQ=255)

                                    cgcgGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAgcgg                                                                                                              >  1:212362/1‑151 (MQ=255)

                                          ttGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGAtt                                                                                                         <  1:473077/150‑1 (MQ=255)

                                          ttGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGAtt                                                                                                         <  1:57168/150‑1 (MQ=255)

                                          ttGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGAtt                                                                                                         <  4:1482067/150‑1 (MQ=255)

                                            gCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTAt                                                                                                       <  1:448021/150‑1 (MQ=255)

                                                   gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCggtg                                                                                               >  4:136953/1‑151 (MQ=255)

                                                   gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCggtg                                                                                               <  4:1686259/151‑1 (MQ=255)

                                                   gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCggtg                                                                                               <  4:186816/151‑1 (MQ=255)

                                                      ggATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCggggg                                                                                              >  1:266863/1‑149 (MQ=255)

                                                       gATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGCGGGA‑t                                                                                          >  4:377071/1‑151 (MQ=255)

                                                        aTTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCgggggggt                                                                                           >  1:1590940/1‑147 (MQ=255)

                                                        aTTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑tt                                                                                         <  1:1760064/151‑1 (MQ=255)

                                                        aTTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑tt                                                                                         <  4:1552966/151‑1 (MQ=255)

                                                        aTTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑tt                                                                                         <  2:470280/151‑1 (MQ=255)

                                                               aTGCAAAAACGTAAGGGCTTTGGGCGCAAGCGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAgcg                                                                                  <  3:88145/151‑1 (MQ=255)

                                                               aTGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAgcg                                                                                  <  3:19694/151‑1 (MQ=255)

                                                               aTGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGcc                                                                                      >  3:59480/1‑147 (MQ=255)

                                                                tGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTggc                                                                                      >  1:1867731/1‑144 (MQ=255)

                                                                tGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGA‑ttttccgcg                                                                                  >  4:1684013/1‑143 (MQ=255)

                                                                 gCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATcggcggcgggttttcccgccc                                                                                  >  2:264252/1‑138 (MQ=255)

                                                                 gCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTT‑TTGGCCgcgc                                                                                 >  1:403172/1‑150 (MQ=255)

                                                                  cAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCgt                                                                               <  4:100904/151‑1 (MQ=255)

                                                                    aaaaCGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCgttg                                                                             <  4:1464699/151‑1 (MQ=255)

                                                                      aaCGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCgttgtt                                                                           <  1:1673485/151‑1 (MQ=255)

                                                                      aaCGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCgttgtt                                                                           <  4:1723463/151‑1 (MQ=255)

                                                                        cGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCggtgtt                                                                           >  4:602302/1‑149 (MQ=255)

                                                                          tAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCCGCGGggtgtt                                                                           >  1:724114/1‑147 (MQ=255)

                                                                           aaGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCg                                                                       <  3:235403/150‑1 (MQ=255)

                                                                            aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGGtgttgt                                                                         >  1:470504/1‑147 (MQ=255)

                                                                             gggCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTG‑TTGCCAGCGCGGtgttgt                                                                         >  2:338625/1‑146 (MQ=255)

                                                                             gggCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTg                                                                    <  1:963087/151‑1 (MQ=255)

                                                                               gCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGgc                                                                  <  1:861883/151‑1 (MQ=255)

                                                                               gCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGG‑TTGCCCGGGCGGtggtgt                                                                         >  3:163258/1‑144 (MQ=255)

                                                                                 tttGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTTCCAGCGCGTTGTTGTTGCTGgcg                                                                 >  4:1803724/1‑150 (MQ=255)

                                                                                     ggCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTAtt                                                            <  4:1297024/151‑1 (MQ=255)

                                                                                       cgcAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGCGGGATTTGCCGGCG‑GTTGTTGTCGCGGGGGCTCtt                                                            >  4:1395076/1‑149 (MQ=255)

                                                                                         cAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACgg                                                        <  1:329832/151‑1 (MQ=255)

                                                                                           aaCGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcg                                                      <  1:743840/151‑1 (MQ=255)

                                                                                           aaCGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGTTGTTGTCGCAGGCGCCATTTCgg                                                        >  4:1925437/1‑149 (MQ=255)

                                                                                              gggAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCCGCGCGGTGTTGTTGCTGGCGCTATTACGGCGCgg                                                   >  4:84779/1‑151 (MQ=255)

                                                                                              gggAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGGTGTTGTCGCTGGCGCTATTACGGCGCgg                                                   >  1:1558693/1‑151 (MQ=255)

                                                                                                gAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCt                                                 <  1:848280/151‑1 (MQ=255)

                                                                                                gAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCt                                                 <  1:1770229/151‑1 (MQ=255)

                                                                                                gAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGTTGTTGTCGCGGGCGCGATTATGGcgcg                                                    >  2:75812/1‑148 (MQ=255)

                                                                                                 aaaTGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTg                                                <  2:97457/151‑1 (MQ=255)

                                                                                                   aTGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGTA‑TTGTCAGCGGGTTGTTTTCGCTGGCGCTATTACGGCGCGGCCgg                                               >  3:120678/1‑150 (MQ=255)

                                                                                                     gCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCa                                             <  1:1398355/150‑1 (MQ=255)

                                                                                                     gCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCCGCGCGGTGTTGTCGCTGGCGCTATTACGGCGCGGCTgg                                               >  4:1607116/1‑148 (MQ=255)

                                                                                                      cTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTTCGGCGCGGGTGGCC‑gg                                          >  2:433416/1‑151 (MQ=255)

                                                                                                         tGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTa                                        <  3:288736/150‑1 (MQ=255)

                                                                                                          gCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAAc                                      <  2:539404/151‑1 (MQ=255)

                                                                                                          gCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGGTGTTGTCGCTGGCGCTATTACGGCGCGGCggggggggaa                                        >  1:581895/1‑143 (MQ=255)

                                                                                                           cGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGG‑TTGCCCGCGCGTTTTTTTCGCCGGGGCTATTTCCGCGCGGGTgggcgggaa                                        >  1:401541/1‑142 (MQ=255)

                                                                                                           cGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTTCCAGCGCGGTGTTGTTGCTGGGGCCATTTCGGCGCGGGTgggcggggac                                       >  1:1216960/1‑142 (MQ=255)

                                                                                                             gggTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGGTGGTTTCGCTGGCGCTATTACGGCGCgggcgggagggaa                                        >  4:916209/1‑136 (MQ=255)

                                                                                                              ggTGATGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCtt                                  <  4:778236/151‑1 (MQ=255)

                                                                                                                  aTGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTAtt                              <  3:324488/151‑1 (MQ=255)

                                                                                                                   tGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTTCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGGA‑GGGAACGCCTtttt                              >  2:370229/1‑150 (MQ=255)

                                                                                                                   tGGAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCAGCGCGGTGTTGTCGCTGGCGCTATTTCGGCGCGGCTGGGA‑GGGAAAGCttttttt                             >  4:1924446/1‑150 (MQ=255)

                                                                                                                    ggAACAGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTg                             <  1:1614877/150‑1 (MQ=255)

                                                                                                                         aGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGa                       <  2:244016/151‑1 (MQ=255)

                                                                                                                         aGACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGa                       <  4:1556657/151‑1 (MQ=255)

                                                                                                                          gACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGAt                      <  4:1167704/151‑1 (MQ=255)

                                                                                                                          gACATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGAt                      <  4:681396/151‑1 (MQ=255)

                                                                                                                           aCATTACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGGTGTTGTAGCTGGCGCTATTACGGCGCGGCGGGGG‑GGGAACACCTTATTTCGCGGGTg                     >  1:510023/1‑151 (MQ=255)

                                                                                                                               tACCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTTCCCGCCCGTTGTTGTCGCCGGGGCTATTTTGGCGCGGGTGGGC‑GGGAAAGCCTTTTTTCGCGGGTGaag                  >  1:1245904/1‑148 (MQ=255)

                                                                                                                                aCCGCTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTTGCAGCGCGTTGTTGTTGCTGGCGCTATTACGGCGCGGGCGGCA‑GGGAAAGCCTTTTTTCGCGGGTGA‑GGcc               >  4:1262585/1‑151 (MQ=255)

                                                                                                                                   tcTCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTTCCCGCGCGGTGTTGTCGCTGGGGCTATTTCGGCGCGGCCGGGA‑GGGAAAGCTTTTTTTTGCGGGTGG‑GGgcccc            >  1:1075923/2‑151 (MQ=255)

                                                                                                                                     tCCCCTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGGTGA‑GGccccccc          >  2:98578/1‑149 (MQ=255)

                                                                                                                                      ccccTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGATGA‑GGCCCCCGc          <  3:264005/150‑1 (MQ=255)

                                                                                                                                      ccccTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGATGA‑GGCCCCCGCa         <  2:204356/151‑1 (MQ=255)

                                                                                                                                      ccccTGCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGATGA‑GGCCCCCGCa         <  2:246324/151‑1 (MQ=255)

                                                                                                                                           gCTCCGTGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGATGA‑GGCCCCCGCACTggg    <  4:1203569/151‑1 (MQ=255)

                                                                                                                                              ccgtgccgtgGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGA‑TTGCCCGAGCGGTTTTTTCGCTGGGGCTATTTCGGCGCGGGTGGCG‑GGGAAAGCCTTTTTTTGCGGGAGA‑GGcccccccccccgggg  >  4:1504703/1‑140 (MQ=255)

                                                                                                                                              ccgtgccgtgGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGTG‑TTGCCCAGGCGTTTGTGTCGCCGGGGCTATTTCGGCGCGGCGGGGATGGTAA‑GGTTTATTTGGCGGGTGAGGGCCCCCCCACTggag   >  4:439628/1‑148 (MQ=255)

ACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTA‑TTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCA‑GGTAACGCTTTATTGCGCGGATGA‑GGCCCCCGCACTGGGTG  >  NC_000913/2442379‑2442670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`