Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,534,187 A→G E41G (GAA→GGA)  ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,534,1870AG99.4% 588.8 / NA 155E41G (GAA→GGA) ptsIPEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (73/81);  total (73/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 155 total aligned reads displayed.

AAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAAT  >  NC_000913/2534039‑2534331
                                                                                                                                                     |                                                                                                                                                
aaaaTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGAc                                                                                                                                                <  1:1788069/151‑1 (MQ=255)
 aaaTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACg                                                                                                                                               <  4:1345976/151‑1 (MQ=255)
 aaaTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACg                                                                                                                                               >  2:490534/1‑151 (MQ=255)
   aTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGtt                                                                                                                                             <  1:727067/151‑1 (MQ=255)
     cAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGtttt                                                                                                                                           <  1:1417517/151‑1 (MQ=255)
      aGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTc                                                                                                                                          >  4:1250438/1‑151 (MQ=255)
         cacaAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGa                                                                                                                                       <  1:480799/151‑1 (MQ=255)
          acaAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGTCCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTg                                                                                                                                        >  1:1378491/1‑149 (MQ=255)
           caAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGc                                                                                                                                     >  1:319713/1‑151 (MQ=255)
                   gTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGc                                                                                                                             <  2:64768/151‑1 (MQ=255)
                   gTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGc                                                                                                                             >  1:54881/1‑151 (MQ=255)
                    tAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            >  4:916036/1‑151 (MQ=255)
                    tAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            >  4:424033/1‑151 (MQ=255)
                     aGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            <  4:1500316/150‑1 (MQ=255)
                     aGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            >  1:546854/1‑150 (MQ=255)
                     aGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTGTCTGAGCGGTCGTGCCa                                                                                                                           <  1:1637572/151‑1 (MQ=255)
                           aTGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCAt                                                                                                                     >  4:598851/1‑151 (MQ=255)
                             gATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATc                                                                                                                    <  1:713498/150‑1 (MQ=255)
                             gATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATc                                                                                                                    >  1:334619/1‑150 (MQ=255)
                             gATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCa                                                                                                                   <  4:209280/151‑1 (MQ=255)
                              aTTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATc                                                                                                                    >  3:226482/1‑149 (MQ=255)
                                ttCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGcc                                                                                                                >  4:301959/1‑151 (MQ=255)
                                ttCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGcc                                                                                                                >  1:981621/1‑151 (MQ=255)
                                  cAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGccc                                                                                                               >  1:202759/1‑150 (MQ=255)
                                  cAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGGTGATCAGGAAGTTGGACGTTTTCTGAGCGGGCGTGCCAAGGCATCAGccc                                                                                                               >  4:38771/1‑150 (MQ=255)
                                   aGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGACAAGGCATAAGc                                                                                                                 >  3:34908/1‑147 (MQ=255)
                                    ggCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGc                                                                                                            <  4:1581558/151‑1 (MQ=255)
                                     gCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCt                                                                                                           >  2:150513/1‑151 (MQ=255)
                                      cATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTg                                                                                                          <  1:741293/151‑1 (MQ=255)
                                      cATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTg                                                                                                          >  4:1393105/1‑151 (MQ=255)
                                       aTTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTgg                                                                                                         >  4:1646170/1‑151 (MQ=255)
                                         tttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGa                                                                                                        >  4:709628/1‑150 (MQ=255)
                                          ttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGaa                                                                                                       <  2:356026/150‑1 (MQ=255)
                                          ttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGaa                                                                                                       <  1:989491/150‑1 (MQ=255)
                                          ttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGaa                                                                                                       <  4:1190742/150‑1 (MQ=255)
                                            aGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGTAAAc                                                                                                     <  4:1474028/150‑1 (MQ=255)
                                            aGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACg                                                                                                    <  1:246163/151‑1 (MQ=255)
                                            aGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACg                                                                                                    <  1:406461/151‑1 (MQ=255)
                                              cATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGAt                                                                                                  <  3:150506/151‑1 (MQ=255)
                                                tCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                                                 >  1:215313/1‑150 (MQ=255)
                                                  cccGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCaa                                                                                               >  1:1475466/1‑150 (MQ=255)
                                                   ccGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCaaaa                                                                                             <  1:1056136/151‑1 (MQ=255)
                                                           cGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                                                     <  4:1246052/151‑1 (MQ=255)
                                                           cGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                                                     <  4:31244/151‑1 (MQ=255)
                                                            gCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTg                                                                                    >  1:1591248/1‑151 (MQ=255)
                                                               ttCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTgg                                                                                   <  4:1530643/149‑1 (MQ=255)
                                                               ttCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGt                                                                                  <  1:1634276/150‑1 (MQ=255)
                                                                tCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                                                 <  4:141085/150‑1 (MQ=255)
                                                                     aaaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGt                                                                           >  4:133694/1‑151 (MQ=255)
                                                                     aaaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGt                                                                           >  2:97466/1‑151 (MQ=255)
                                                                      aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                                                          <  4:1186488/151‑1 (MQ=255)
                                                                      aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                                                          <  1:294884/151‑1 (MQ=255)
                                                                      aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                                                          >  1:1720978/1‑151 (MQ=255)
                                                                       aGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTc                                                                         <  1:1317159/151‑1 (MQ=255)
                                                                        gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                                                        <  4:1713647/151‑1 (MQ=255)
                                                                           ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     >  1:133325/1‑151 (MQ=255)
                                                                            tGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     >  1:488610/1‑150 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                                                      <  1:1698373/148‑1 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTga                                                                    >  1:1004665/1‑150 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     <  4:749279/149‑1 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     <  4:482950/149‑1 (MQ=255)
                                                                               ttCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTga                                                                    >  4:1062989/1‑148 (MQ=255)
                                                                                tttggaaaaagaCCGAATTGTTATTTACCCGAAAAAAAATTCTGCCCACCCAGGTGGATAGGGAAGTTGACCGTTTTCGAGGCGGCGGGGCAAAGCCTTCAGCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                                                      <  1:414753/137‑1 (MQ=255)
                                                                                  tGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                                                >  1:346546/1‑149 (MQ=255)
                                                                                   gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                                                <  2:413587/148‑1 (MQ=255)
                                                                                   gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaa                                                              <  3:43562/150‑1 (MQ=255)
                                                                                    aaaGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaa                                                              >  1:1446154/1‑149 (MQ=255)
                                                                                    aaaGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaag                                                            <  3:498439/151‑1 (MQ=255)
                                                                                     aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaaga                                                           <  1:185102/151‑1 (MQ=255)
                                                                                      agaagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaa                                                          <  3:392906/151‑1 (MQ=255)
                                                                                         agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                                                       >  1:1023666/1‑151 (MQ=255)
                                                                                             gAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCt                                                   <  2:246956/151‑1 (MQ=255)
                                                                                                 ttGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                                                >  4:195618/1‑150 (MQ=255)
                                                                                                 ttGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                                                >  3:356193/1‑150 (MQ=255)
                                                                                                  tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAGAAGCCATCctt                                                 >  4:1908915/1‑147 (MQ=255)
                                                                                                   gTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGa                                               >  3:516487/1‑149 (MQ=255)
                                                                                                    tCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAgg                                            >  1:1779158/1‑151 (MQ=255)
                                                                                                      aTTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGc                                          <  1:169925/151‑1 (MQ=255)
                                                                                                       ttGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGc                                          >  3:545047/1‑150 (MQ=255)
                                                                                                        tGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa                                         <  3:64558/150‑1 (MQ=255)
                                                                                                        tGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa                                         <  1:1054963/150‑1 (MQ=255)
                                                                                                         gACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCat                                        <  4:330061/150‑1 (MQ=255)
                                                                                                         gACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCat                                        >  1:942776/1‑150 (MQ=255)
                                                                                                           ccGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCAGCTTTGAAGGGCAtatt                                     <  3:163871/151‑1 (MQ=255)
                                                                                                            cGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta                                    <  4:60364/151‑1 (MQ=255)
                                                                                                            cGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta                                    <  4:851681/151‑1 (MQ=255)
                                                                                                            cGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta                                    <  1:717622/151‑1 (MQ=255)
                                                                                                              gAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtg                                  <  4:796250/151‑1 (MQ=255)
                                                                                                               aaaaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAGACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc                                 >  3:236121/1‑151 (MQ=255)
                                                                                                                   aaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATgctgct                             >  1:444153/1‑151 (MQ=255)
                                                                                                                          tGCCGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGa                          >  3:375181/1‑147 (MQ=255)
                                                                                                                             cGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgag                   <  3:108377/151‑1 (MQ=255)
                                                                                                                             cGACCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATg                     >  4:1288406/1‑149 (MQ=255)
                                                                                                                                 ccAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgagga                 <  4:491699/150‑1 (MQ=255)
                                                                                                                                 ccAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgagg                  >  1:250765/1‑149 (MQ=255)
                                                                                                                                   aGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCt              >  4:1006217/1‑151 (MQ=255)
                                                                                                                                          tCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGc         >  1:1912085/1‑149 (MQ=255)
                                                                                                                                          tCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGc         >  2:425685/1‑149 (MQ=255)
                                                                                                                                           cAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAg       <  2:526385/150‑1 (MQ=255)
                                                                                                                                               aaGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAAt  <  2:39507/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
AAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAAT  >  NC_000913/2534039‑2534331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: