Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,539,372 T→C N71S (AAC→AGC)  cysM ← cysteine synthase B (O‑acetylserine sulfhydrolase B)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,539,3720TC100.0% 547.3 / NA 147N71S (AAC→AGC) cysMcysteine synthase B (O‑acetylserine sulfhydrolase B)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (72/75);  total (72/75)

Only 100 of 149 total aligned reads displayed.

TCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTAT  >  NC_000913/2539225‑2539519
                                                                                                                                                   |                                                                                                                                                   
tCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTAc                                                                                                                                                  >  3:103167/1‑151 (MQ=255)
  cATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTAcc                                                                                                                                                 >  4:1749283/1‑150 (MQ=255)
      cccTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCa                                                                                                                                                >  1:593785/1‑147 (MQ=255)
       ccTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACtgg                                                                                                                                            >  2:422909/1‑150 (MQ=255)
         tGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACtggtgg                                                                                                                                         >  1:136899/1‑151 (MQ=255)
          gCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACtggtg                                                                                                                                          <  3:267831/149‑1 (MQ=255)
          gCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGc                                                                                                                                        >  1:1062486/1‑151 (MQ=255)
            tctTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCtt                                                                                                                                      >  3:269131/1‑151 (MQ=255)
            tctTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCtt                                                                                                                                      >  4:88142/1‑151 (MQ=255)
              tttGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCg                                                                                                                                    >  1:331079/1‑151 (MQ=255)
                tGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTc                                                                                                                                     <  1:1147450/148‑1 (MQ=255)
                tGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGAt                                                                                                                                  >  4:847129/1‑151 (MQ=255)
                 ggTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGAtt                                                                                                                                 <  1:1169051/151‑1 (MQ=255)
                  gTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGAtt                                                                                                                                 >  4:429126/1‑150 (MQ=255)
                  gTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTa                                                                                                                                <  4:783078/151‑1 (MQ=255)
                     aCAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTa                                                                                                                                >  4:734163/1‑148 (MQ=255)
                      cAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGTCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGAc                                                                                                                            >  1:1091445/1‑151 (MQ=255)
                       aaGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGAc                                                                                                                            >  4:581704/1‑150 (MQ=255)
                        aGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACAt                                                                                                                          <  2:103168/151‑1 (MQ=255)
                         gAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATc                                                                                                                         <  1:830808/151‑1 (MQ=255)
                              aGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCg                                                                                                                    >  2:40715/1‑151 (MQ=255)
                               gTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTTAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCGCCCg                                                                                                                    <  4:658774/150‑1 (MQ=255)
                                ttCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGt                                                                                                                  >  4:1460241/1‑151 (MQ=255)
                                ttCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGt                                                                                                                  >  1:140578/1‑151 (MQ=255)
                                 tCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGtt                                                                                                                 >  1:869585/1‑151 (MQ=255)
                                 tCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGt                                                                                                                  <  1:67166/150‑1 (MQ=255)
                                 tCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGt                                                                                                                  <  4:1493438/150‑1 (MQ=255)
                                   cGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTa                                                                                                               <  2:161473/151‑1 (MQ=255)
                                        cATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTc                                                                                                          >  4:298503/1‑151 (MQ=255)
                                        cATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTc                                                                                                          >  1:689314/1‑151 (MQ=255)
                                         aTAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCGCCCGGTTTAATTTcc                                                                                                         >  4:1865399/1‑151 (MQ=255)
                                         aTAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTcc                                                                                                         <  1:902401/151‑1 (MQ=255)
                                           aaGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTcccc                                                                                                       <  3:338676/151‑1 (MQ=255)
                                            aGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCcg                                                                                                      >  4:538383/1‑151 (MQ=255)
                                            aGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCcg                                                                                                      <  4:612761/151‑1 (MQ=255)
                                            aGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCcg                                                                                                      >  1:32771/1‑151 (MQ=255)
                                              cACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCcgcg                                                                                                    <  4:570833/151‑1 (MQ=255)
                                               aCGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCgcgc                                                                                                   >  4:1886944/1‑151 (MQ=255)
                                                cGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCt                                                                                                  >  4:1486497/1‑151 (MQ=255)
                                                   aTCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCtttt                                                                                               <  1:476684/151‑1 (MQ=255)
                                                     cgccgcACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTcc                                                                                             >  1:1198947/1‑151 (MQ=255)
                                                      gccgcACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCg                                                                                            <  2:122082/151‑1 (MQ=255)
                                                        cgcACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGcc                                                                                          >  1:1773169/1‑151 (MQ=255)
                                                        cgcACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGcc                                                                                          >  1:1758175/1‑151 (MQ=255)
                                                        cgcACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGcc                                                                                          >  4:1243429/1‑151 (MQ=255)
                                                          cACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTc                                                                                        >  4:1627042/1‑151 (MQ=255)
                                                            cGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTcg                                                                                       <  1:1195848/150‑1 (MQ=255)
                                                                 ttCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGat                                                                                  <  1:873166/150‑1 (MQ=255)
                                                                  tCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGTCGatc                                                                                 >  1:946094/1‑150 (MQ=255)
                                                                  tCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGatc                                                                                 <  1:451094/150‑1 (MQ=255)
                                                                  tCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGatc                                                                                 >  1:1537683/1‑150 (MQ=255)
                                                                   ccTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGatcat                                                                               <  4:898591/151‑1 (MQ=255)
                                                                   ccTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGatca                                                                                <  4:1063246/150‑1 (MQ=255)
                                                                       gCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGaa                                                                           <  3:376491/151‑1 (MQ=255)
                                                                           aTGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCGATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAg                                                                         <  4:838390/149‑1 (MQ=255)
                                                                           aTGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTg                                                                       >  4:1336992/1‑151 (MQ=255)
                                                                            tgttgtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGc                                                                      >  4:927062/1‑151 (MQ=255)
                                                                                gtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGc                                                                   <  4:1017255/150‑1 (MQ=255)
                                                                                     gCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATc                                                             <  3:73771/151‑1 (MQ=255)
                                                                                        tCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCttt                                                          >  4:1414955/1‑151 (MQ=255)
                                                                                            cAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCaac                                                      <  1:369492/151‑3 (MQ=255)
                                                                                                 tCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAAcc                                                 >  4:1631295/1‑151 (MQ=255)
                                                                                                 tCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAAc                                                  <  3:371802/150‑1 (MQ=255)
                                                                                                   aTGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCt                                                <  4:107330/150‑1 (MQ=255)
                                                                                                    tGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGc                                              >  2:116545/1‑151 (MQ=255)
                                                                                                    tGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGc                                              >  2:225422/1‑151 (MQ=255)
                                                                                                     gCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGc                                              <  1:1695624/150‑1 (MQ=255)
                                                                                                      cGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCg                                            <  1:733844/151‑1 (MQ=255)
                                                                                                      cGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCg                                            >  2:539774/1‑151 (MQ=255)
                                                                                                       gATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCgg                                           <  3:453663/151‑1 (MQ=255)
                                                                                                          aGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGtt                                        <  4:1277713/151‑1 (MQ=255)
                                                                                                          aGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGt                                         <  4:1017148/150‑1 (MQ=255)
                                                                                                           gCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGtt                                        >  1:1190207/1‑150 (MQ=255)
                                                                                                           gCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTa                                       <  1:256665/151‑1 (MQ=255)
                                                                                                            ccTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTAt                                      <  4:455397/151‑1 (MQ=255)
                                                                                                            ccTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTAt                                      >  2:139069/1‑151 (MQ=255)
                                                                                                               ttCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTAtt                                     <  3:362688/149‑1 (MQ=255)
                                                                                                                tCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGcc                                  >  1:1583388/1‑151 (MQ=255)
                                                                                                                 cAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCt                                 >  1:167216/1‑151 (MQ=255)
                                                                                                                 cAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCt                                 >  1:379840/1‑151 (MQ=255)
                                                                                                                 cAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCt                                 >  4:1829039/1‑151 (MQ=255)
                                                                                                                  aGCGCGGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCtt                                <  4:1473252/151‑1 (MQ=255)
                                                                                                                      cgGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCa                             <  1:1050818/150‑1 (MQ=255)
                                                                                                                      cgGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAg                            <  1:1874652/151‑1 (MQ=255)
                                                                                                                      cgGCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAg                            <  4:1149796/151‑1 (MQ=255)
                                                                                                                       ggCAATCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGt                           >  4:400531/1‑151 (MQ=255)
                                                                                                                          aaTCATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGtttt                        <  1:261497/151‑1 (MQ=255)
                                                                                                                             cATTGCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAAc                     >  3:521579/1‑151 (MQ=255)
                                                                                                                                 gCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACcac                  <  4:686060/150‑1 (MQ=255)
                                                                                                                                 gCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACcac                  <  1:1901991/150‑1 (MQ=255)
                                                                                                                                 gCCAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACcac                  <  2:307689/150‑1 (MQ=255)
                                                                                                                                   cAGCGCAATGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACt               >  4:90848/1‑151 (MQ=255)
                                                                                                                                          aTGCCGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGc        >  4:524675/1‑151 (MQ=255)
                                                                                                                                             ccGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGt     >  3:462880/1‑151 (MQ=255)
                                                                                                                                              cGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGtt    <  4:1278104/151‑1 (MQ=255)
                                                                                                                                              cGGTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGtt    <  1:115264/151‑1 (MQ=255)
                                                                                                                                               ggTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTa   <  4:322172/151‑1 (MQ=255)
                                                                                                                                               ggTGCTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTa   <  3:265180/151‑1 (MQ=255)
                                                                                                                                                gtgcTACCACTGGTGGTTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTAt  <  2:21375/151‑1 (MQ=255)
                                                                                                                                                gtgcTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTAt  <  1:773779/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTAT  >  NC_000913/2539225‑2539519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑