Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,589,108 A→G H505R (CAT→CGT)  acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,589,1080AG100.0% 574.6 / NA 153H505R (CAT→CGT) acrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (68/85);  total (68/85)

Only 100 of 158 total aligned reads displayed.

ATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCT  >  NC_000913/2588960‑2589255
                                                                                                                                                    |                                                                                                                                                   
  ggCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGcgtca                                                                                                                                                  <  4:245839/150‑1 (MQ=255)
  ggCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGcgtca                                                                                                                                                  <  1:1373129/150‑1 (MQ=255)
   gCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATg                                                                                                                                                >  1:1612102/1‑151 (MQ=255)
    ccTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATgg                                                                                                                                               <  1:1460535/151‑1 (MQ=255)
       tcttcGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATggg                                                                                                                                              <  2:522058/149‑1 (MQ=255)
       tcttcGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGc                                                                                                                                             <  4:1796515/150‑1 (MQ=255)
         ttcGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCaa                                                                                                                                           >  1:1398666/1‑150 (MQ=255)
         ttcGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCaa                                                                                                                                           >  4:425293/1‑150 (MQ=255)
          tcGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCaaa                                                                                                                                          >  1:1849326/1‑150 (MQ=255)
          tcGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCaa                                                                                                                                           <  2:173113/149‑1 (MQ=255)
            ggcggcACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCaaaa                                                                                                                                         >  1:639082/1‑149 (MQ=255)
              cggcACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGATCGTCATGGGCAAAAAGGc                                                                                                                                     <  1:1482883/151‑1 (MQ=255)
                gcACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCtt                                                                                                                                   <  4:362077/151‑1 (MQ=255)
                   ccaccGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCttttt                                                                                                                                <  2:12494/151‑1 (MQ=255)
                    caccGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCtttttt                                                                                                                               <  2:91928/151‑1 (MQ=255)
                      ccGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGc                                                                                                                             >  4:1327059/1‑151 (MQ=255)
                        ggTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCt                                                                                                                           >  4:571237/1‑151 (MQ=255)
                          tGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTgg                                                                                                                         >  2:54483/1‑151 (MQ=255)
                           gCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTgg                                                                                                                         <  1:1894383/150‑1 (MQ=255)
                             cATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGctt                                                                                                                      <  3:227125/151‑1 (MQ=255)
                                cTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAAc                                                                                                                   >  4:171075/1‑151 (MQ=255)
                                  aTCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTaa                                                                                                                    <  4:826051/148‑1 (MQ=255)
                                  aTCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAAc                                                                                                                   <  1:508034/149‑1 (MQ=255)
                                     gCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAAcc                                                                                                                  >  4:791237/1‑147 (MQ=255)
                                     gCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGAt                                                                                                              <  4:211360/151‑1 (MQ=255)
                                         gTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGttt                                                                                                          <  1:125794/151‑1 (MQ=255)
                                           tctcTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTa                                                                                                         <  2:468699/150‑1 (MQ=255)
                                           tctcTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTa                                                                                                         <  1:1155049/150‑1 (MQ=255)
                                           tctcTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTa                                                                                                         <  1:1556762/150‑1 (MQ=255)
                                            ctctATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAAc                                                                                                       >  3:153062/1‑151 (MQ=255)
                                            ctctATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAAc                                                                                                       >  1:325335/1‑151 (MQ=255)
                                             tctATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTTTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAGAAAAGCTTTTTTGCCTGGTTTAACCAAATGTTTa                                                                                                         <  3:86747/148‑1 (MQ=255)
                                             tctATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAAcc                                                                                                      >  4:444567/1‑151 (MQ=255)
                                             tctATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAAcc                                                                                                      >  1:1826797/1‑151 (MQ=255)
                                               tATTACCATTGTTGCGGCGATGGTGATGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTAATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGc                                                                                                    >  3:288262/1‑151 (MQ=255)
                                                aTTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGc                                                                                                    <  3:258733/150‑1 (MQ=255)
                                                  tACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAAc                                                                                                 >  3:236438/1‑151 (MQ=255)
                                                   aCCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACg                                                                                                >  4:338192/1‑151 (MQ=255)
                                                   aCCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACg                                                                                                <  4:1669941/151‑1 (MQ=255)
                                                     cATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGcc                                                                                              >  3:483678/1‑151 (MQ=255)
                                                         gttgCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGaa                                                                                           <  3:5145/150‑1 (MQ=255)
                                                         gttgCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAAc                                                                                          >  3:281246/1‑151 (MQ=255)
                                                          ttgCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAAc                                                                                          <  4:136777/150‑1 (MQ=255)
                                                            gcggcgATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCt                                                                                       <  4:278853/151‑1 (MQ=255)
                                                             cggcgATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTa                                                                                      <  4:606112/151‑1 (MQ=255)
                                                              ggcgATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGGAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTAc                                                                                     >  2:433817/1‑151 (MQ=255)
                                                              ggcgATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTa                                                                                      <  4:1829056/150‑1 (MQ=255)
                                                               gcgATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACg                                                                                    <  1:931038/151‑1 (MQ=255)
                                                                 gATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGa                                                                                   >  4:328239/1‑150 (MQ=255)
                                                                 gATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGa                                                                                   >  4:401550/1‑150 (MQ=255)
                                                                 gATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGAGAGCGTCATGTCCAAAAAGGCTTTTTTGCCTGATTTAACCAGATGTTTAACCGCAACGCCGAACGCTAc                                                                                     >  3:120455/1‑148 (MQ=255)
                                                                       gCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAggg                                                                            <  3:166894/151‑1 (MQ=255)
                                                                          gTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTg                                                                         <  4:1760044/151‑1 (MQ=255)
                                                                             aGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCg                                                                      >  4:774366/1‑151 (MQ=255)
                                                                               tACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCgt                                                                     <  4:1215530/150‑2 (MQ=255)
                                                                                aCTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGaaa                                                                   <  2:307014/151‑1 (MQ=255)
                                                                                 cTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGaaa                                                                   >  1:1555042/1‑150 (MQ=255)
                                                                                 cTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGaaa                                                                   >  1:1263074/1‑150 (MQ=255)
                                                                                   ggTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAAtt                                                                <  4:1458451/151‑1 (MQ=255)
                                                                                   ggTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAAt                                                                 <  4:1734838/150‑1 (MQ=255)
                                                                                      aGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATtct                                                              <  4:715872/150‑1 (MQ=255)
                                                                                      aGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATtct                                                              <  1:1142637/150‑1 (MQ=255)
                                                                                       gCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTcc                                                            <  4:1833550/151‑1 (MQ=255)
                                                                                       gCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTcc                                                            >  1:1008826/1‑151 (MQ=255)
                                                                                        cGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCa                                                           <  1:917440/151‑1 (MQ=255)
                                                                                            gatCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCg                                                        <  1:699765/150‑1 (MQ=255)
                                                                                             atCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGt                                                       >  1:213498/1‑150 (MQ=255)
                                                                                                cTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGcc                                                   >  4:733598/1‑151 (MQ=255)
                                                                                                  cACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGcc                                                   <  2:246014/149‑1 (MQ=255)
                                                                                                  cACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCt                                                  <  4:473186/150‑1 (MQ=255)
                                                                                                   aCTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTgc                                                >  4:885086/1‑151 (MQ=255)
                                                                                                   aCTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTgc                                                >  4:1056920/1‑151 (MQ=255)
                                                                                                            ctGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGAt                                         <  1:501703/149‑1 (MQ=255)
                                                                                                              gtgtgCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATtgtg                                     <  4:1268599/151‑1 (MQ=255)
                                                                                                              gtgtgCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATtgtg                                     <  1:444410/151‑1 (MQ=255)
                                                                                                              gtgtgCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATtg                                       <  2:153218/149‑1 (MQ=255)
                                                                                                                  gCCACACTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGAttt                                 >  1:1415014/1‑151 (MQ=255)
                                                                                                                    cacaCTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTa                                <  1:59580/150‑1 (MQ=255)
                                                                                                                    cacaCTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTAt                               <  3:54483/151‑1 (MQ=255)
                                                                                                                    cacaCTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGT‑GCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATg                              <  1:39387/151‑1 (MQ=255)
                                                                                                                      cacTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGt                             >  4:792163/1‑151 (MQ=255)
                                                                                                                      cacTACTTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGt                             <  1:171025/151‑1 (MQ=255)
                                                                                                                        ctactTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAg                                                                              <  2:380298/100‑1 (MQ=255)
                                                                                                                        ctactTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAg                                                                              >  3:380285/1‑100 (MQ=255)
                                                                                                                         tactTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCct                          >  2:105131/1‑151 (MQ=255)
                                                                                                                           ctTAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCctgc                        >  1:1622519/1‑151 (MQ=255)
                                                                                                                             tAAGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCctgctg                      >  1:694212/1‑151 (MQ=255)
                                                                                                                              aaGCCACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCctgctg                      <  1:1747710/150‑1 (MQ=255)
                                                                                                                                 ccACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTg                  >  1:1040789/1‑151 (MQ=255)
                                                                                                                                 ccACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTg                  >  1:259072/1‑151 (MQ=255)
                                                                                                                                 ccACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTg                  >  1:1778786/1‑151 (MQ=255)
                                                                                                                                  cACTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTgg                 <  2:531487/151‑1 (MQ=255)
                                                                                                                                    cTGAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTggcg               >  4:1444862/1‑151 (MQ=255)
                                                                                                                                      gAAAAAAGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTggcggc             >  1:916291/1‑151 (MQ=255)
                                                                                                                                          aaaGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATgg         >  4:1108725/1‑151 (MQ=255)
                                                                                                                                          aaaGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATgg         <  1:356002/151‑1 (MQ=255)
                                                                                                                                            aGGTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGgtg       >  1:557623/1‑151 (MQ=255)
                                                                                                                                             ggTGAGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGgtgt      <  4:92086/151‑1 (MQ=255)
                                                                                                                                                 aGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGtgttcct  <  4:1140540/151‑1 (MQ=255)
                                                                                                                                                 aGCGTCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGtgttcct  >  1:450194/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAATTCTCCACCGTAGCCTGCGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCT  >  NC_000913/2588960‑2589255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑