BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,609,458	T→C	R324R (CGT→CGC)	hyfG →	hydrogenase 4, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,609,458	0	T	C	100.0%	497.1 / NA	134	R324R (CGT→CGC)	hyfG	hydrogenase 4, subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (71/63); total (71/63)

Only 100 of 136 total aligned reads displayed.

GATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAA  >  NC_000913/2609311‑2609605

gATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCaaa                                                                                                                                                  <  1:1370696/151‑1 (MQ=255)

 aTTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCaaa                                                                                                                                                  >  1:293196/1‑150 (MQ=255)

   tCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAAcc                                                                                                                                               >  1:945964/1‑151 (MQ=255)

    ctgctgGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCt                                                                                                                                              >  1:1528778/1‑151 (MQ=255)

      gctgGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTa                                                                                                                                             <  1:1273600/150‑1 (MQ=255)

        tgGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACgg                                                                                                                                          >  3:386748/1‑151 (MQ=255)

         ggAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACg                                                                                                                                           <  3:13292/149‑1 (MQ=255)

           aaGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGTCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCt                                                                                                                                       <  1:1173193/151‑1 (MQ=255)

           aaGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCt                                                                                                                                       <  2:153426/151‑1 (MQ=255)

            aGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTg                                                                                                                                      <  4:1141333/151‑1 (MQ=255)

             gTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGa                                                                                                                                     <  2:202535/151‑1 (MQ=255)

             gTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGa                                                                                                                                     <  1:291496/151‑1 (MQ=255)

              tCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGa                                                                                                                                     >  3:407642/1‑150 (MQ=255)

               cGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAAt                                                                                                                                   <  4:369998/151‑1 (MQ=255)

               cGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAAt                                                                                                                                   >  1:1063933/1‑151 (MQ=255)

                 aaCGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCt                                                                                                                                 <  2:545638/151‑1 (MQ=255)

                 aaCGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCt                                                                                                                                 >  3:126991/1‑151 (MQ=255)

                 aaCGGCTACACAGTCATTTGCTTAACCTTGGCCCCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATc                                                                                                                                  <  1:1884591/150‑1 (MQ=255)

                   cGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGa                                                                                                                               <  4:20000/151‑1 (MQ=255)

                    ggCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGAt                                                                                                                              >  1:1783470/1‑151 (MQ=255)

                      cTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAATTCGTTGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTTAATCTGAtt                                                                                                                             >  2:296189/1‑150 (MQ=255)

                        acacAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATtggt                                                                                                                          >  4:1246263/1‑151 (MQ=255)

                         cacaGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATtggtg                                                                                                                         <  3:231369/151‑1 (MQ=255)

                          acaGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATtggtgg                                                                                                                        >  1:654632/1‑151 (MQ=255)

                            aGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGgtggtg                                                                                                                      >  2:280212/1‑151 (MQ=255)

                            aGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGgtggtg                                                                                                                      >  1:1220770/1‑151 (MQ=255)

                               cATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGtt                                                                                                                    <  1:1419571/150‑1 (MQ=255)

                               cATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGtt                                                                                                                    <  2:215584/150‑1 (MQ=255)

                               cATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTc                                                                                                                   <  4:425787/151‑1 (MQ=255)

                               cATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTc                                                                                                                   >  1:1024089/1‑151 (MQ=255)

                               cATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTc                                                                                                                   >  1:878023/1‑151 (MQ=255)

                                aTTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTGCGGTCTGAATCTGATTGGTGGTGTTcg                                                                                                                  <  1:484717/151‑1 (MQ=255)

                                    gCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcgccgc                                                                                                              <  1:1301368/151‑1 (MQ=255)

                                     cTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCcgcg                                                                                                             >  2:454953/1‑151 (MQ=255)

                                      ttAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCcgcg                                                                                                             >  4:1809596/1‑150 (MQ=255)

                                      ttAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCcgcg                                                                                                             >  4:1783742/1‑150 (MQ=255)

                                         aCCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGatat                                                                                                         >  4:454185/1‑151 (MQ=255)

                                           cTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtc                                                                                                       >  1:1117779/1‑151 (MQ=255)

                                              ggCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCa                                                                                                    <  1:793554/151‑1 (MQ=255)

                                              ggCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCa                                                                                                    <  1:88613/151‑1 (MQ=255)

                                              ggCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCa                                                                                                    <  4:1492079/151‑1 (MQ=255)

                                                   ctcCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAag                                                                                                 <  4:77221/149‑1 (MQ=255)

                                                     ccTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGc                                                                                              <  1:343560/150‑1 (MQ=255)

                                                     ccTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGc                                                                                              <  1:198481/150‑1 (MQ=255)

                                                         ccATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGt                                                                                         >  4:5516/1‑151 (MQ=255)

                                                              tCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGc                                                                                     <  2:145561/150‑1 (MQ=255)

                                                               cGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCa                                                                                    >  1:158669/1‑150 (MQ=255)

                                                                gTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCaa                                                                                   >  4:669733/1‑150 (MQ=255)

                                                                gTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCaa                                                                                   >  1:611171/1‑150 (MQ=255)

                                                                  tGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACg                                                                                >  4:533495/1‑151 (MQ=255)

                                                                   ggTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACg                                                                                <  1:1178937/150‑1 (MQ=255)

                                                                    gTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCt                                                                              <  4:1878630/151‑1 (MQ=255)

                                                                    gTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCt                                                                              <  1:262530/151‑1 (MQ=255)

                                                                     ttttGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTg                                                                             >  3:239149/1‑151 (MQ=255)

                                                                      tttGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTg                                                                             >  1:503325/1‑150 (MQ=255)

                                                                        tGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGaa                                                                           >  3:215251/1‑150 (MQ=255)

                                                                         gATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGaaa                                                                          <  1:382860/150‑1 (MQ=255)

                                                                          aTACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACt                                                                        <  4:1316154/151‑1 (MQ=255)

                                                                          aTACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACt                                                                        <  4:1677079/151‑1 (MQ=255)

                                                                           tACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTg                                                                       >  1:998562/1‑151 (MQ=255)

                                                                           tACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTg                                                                       >  1:39377/1‑151 (MQ=255)

                                                                            aCCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTgg                                                                      >  4:180692/1‑151 (MQ=255)

                                                                                   ttATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTgcgc                                                                 <  1:1588819/149‑1 (MQ=255)

                                                                                   ttATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGcgcg                                                                >  4:369309/1‑150 (MQ=255)

                                                                                   ttATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGcgcg                                                                <  1:1725462/150‑1 (MQ=255)

                                                                                   ttATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGcgcg                                                                <  1:83492/150‑1 (MQ=255)

                                                                                     aTGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCgaga                                                             <  4:244594/151‑1 (MQ=255)

                                                                                        cAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgc                                                          >  4:698663/1‑151 (MQ=255)

                                                                                        cAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgc                                                          >  4:269398/1‑151 (MQ=255)

                                                                                        cAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgc                                                          >  1:1518376/1‑151 (MQ=255)

                                                                                         aaTTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcg                                                         <  4:198369/151‑1 (MQ=255)

                                                                                         aaTTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcg                                                         >  1:1829129/1‑151 (MQ=255)

                                                                                          aTTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcgc                                                        >  3:183416/1‑151 (MQ=255)

                                                                                           tttttCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcgcg                                                       >  1:1328961/1‑151 (MQ=255)

                                                                                                   cgTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACgtg                                               >  4:1644540/1‑151 (MQ=255)

                                                                                                    gTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACgtgt                                              <  4:339711/151‑1 (MQ=255)

                                                                                                    gTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACgtgt                                              <  1:663364/151‑1 (MQ=255)

                                                                                                        gggAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                           >  2:311838/1‑150 (MQ=255)

                                                                                                        gggAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                           >  2:198817/1‑150 (MQ=255)

                                                                                                        gggAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                           >  1:796292/1‑150 (MQ=255)

                                                                                                         ggAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                           >  3:486770/1‑149 (MQ=255)

                                                                                                         ggAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                           >  3:89580/1‑149 (MQ=255)

                                                                                                           aaaaGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCt                                       <  4:1544553/151‑1 (MQ=255)

                                                                                                                tCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGt                                    >  4:881992/1‑149 (MQ=255)

                                                                                                                 cGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTaga                                 <  3:456502/151‑1 (MQ=255)

                                                                                                                 cGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTag                                  <  1:163664/150‑1 (MQ=255)

                                                                                                                     gACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGAt                              <  1:574871/150‑1 (MQ=255)

                                                                                                                     gACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGAt                              <  4:654906/150‑1 (MQ=255)

                                                                                                                        gATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGAtgctg                          >  4:1262872/1‑151 (MQ=255)

                                                                                                                         aTGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGAtgctgc                         <  1:1062858/151‑1 (MQ=255)

                                                                                                                           ggCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCtgctt                       <  4:1466927/151‑1 (MQ=255)

                                                                                                                             cGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCtgcttgc                     <  2:14255/151‑1 (MQ=255)

                                                                                                                               gAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCtgcttgct                    >  4:1104565/1‑150 (MQ=255)

                                                                                                                                 aTTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACg                 <  1:1466582/151‑1 (MQ=255)

                                                                                                                                 aTTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACg                 <  1:1148939/151‑1 (MQ=255)

                                                                                                                                  ttGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGc                >  4:433686/1‑151 (MQ=255)

                                                                                                                                     cTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCg              >  1:1083557/1‑150 (MQ=255)

                                                                                                                                       gATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAat           <  3:347232/151‑1 (MQ=255)

                                                                                                                                          cGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATg        <  4:439564/151‑1 (MQ=255)

                                                                                                                                                gcgcAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGaaca   >  2:407970/1‑150 (MQ=255)

GATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAA  >  NC_000913/2609311‑2609605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`