Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 261,402 G→A A339T (GCC→ACC)  proB → gamma‑glutamate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913261,4020GA99.2% 477.3 / ‑4.6 127A339T (GCC→ACC) proBgamma‑glutamate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (68/58);  total (69/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 127 total aligned reads displayed.

TGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATT  >  NC_000913/261253‑261549
                                                                                                                                                     |                                                                                                                                                   
tGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGc                                                                                                                                                    >  4:1744078/1‑151 (MQ=255)
  gAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCg                                                                                                                                                  <  1:635326/151‑1 (MQ=255)
   aaCGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCgg                                                                                                                                                 <  1:1590881/151‑1 (MQ=255)
   aaCGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCgg                                                                                                                                                 >  3:116445/1‑151 (MQ=255)
       cgGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGacac                                                                                                                                             >  1:238527/1‑151 (MQ=255)
        ggCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGAcacc                                                                                                                                            >  4:254559/1‑151 (MQ=255)
          cAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGAcaccac                                                                                                                                          >  1:612350/1‑151 (MQ=255)
          cAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGAcaccac                                                                                                                                          <  4:154094/151‑1 (MQ=255)
            gCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTc                                                                                                                                        >  4:1396882/1‑151 (MQ=255)
               cccTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGc                                                                                                                                      >  4:1056115/1‑150 (MQ=255)
                ccTGTTGCCTAACGGCATTAAAAGCGTGACGGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACCGCGTCAGTCGTTACAACACCGATGCATTACGCCGTATTACCGGACACCACTCGCaa                                                                                                                                    <  3:135474/151‑1 (MQ=255)
                ccTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCaa                                                                                                                                    <  3:532780/151‑1 (MQ=255)
                ccTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCaa                                                                                                                                    <  3:289522/151‑1 (MQ=255)
                 cTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGCATTACCGGACACCACTCGc                                                                                                                                      >  1:1259939/1‑148 (MQ=255)
                   gTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAg                                                                                                                                   <  1:747634/149‑1 (MQ=255)
                      gCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAAtt                                                                                                                              <  4:130874/151‑1 (MQ=255)
                      gCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAAtt                                                                                                                              <  4:296695/151‑1 (MQ=255)
                          aaaGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCTCCCACGGAGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATg                                                                                                                          >  1:1499871/1‑151 (MQ=255)
                           aaGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGc                                                                                                                         <  4:1570292/151‑1 (MQ=255)
                            aGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGc                                                                                                                         >  4:949703/1‑150 (MQ=255)
                            aGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGc                                                                                                                         >  4:1732098/1‑150 (MQ=255)
                             ggCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCaa                                                                                                                       <  4:697099/151‑1 (MQ=255)
                              gCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAAt                                                                                                                      <  4:1065239/151‑1 (MQ=255)
                               cATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAAt                                                                                                                      >  2:64374/1‑150 (MQ=255)
                               cATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATa                                                                                                                     <  1:1247221/151‑1 (MQ=255)
                                 ttAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACt                                                                                                                   >  1:1707136/1‑151 (MQ=255)
                                 ttAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACt                                                                                                                   >  3:438012/1‑151 (MQ=255)
                                  tAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTg                                                                                                                  >  1:1688226/1‑151 (MQ=255)
                                  tAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTg                                                                                                                  >  4:874801/1‑151 (MQ=255)
                                   aaaaGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTgg                                                                                                                 <  4:689911/151‑1 (MQ=255)
                                    aaaGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTggg                                                                                                                >  4:558368/1‑151 (MQ=255)
                                     aaGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTggg                                                                                                                >  1:171078/1‑150 (MQ=255)
                                     aaGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGa                                                                                                               <  4:238603/151‑1 (MQ=255)
                                        cGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGatat                                                                                                            >  2:341618/1‑151 (MQ=255)
                                         gTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATg                                                                                                           <  4:1898011/151‑1 (MQ=255)
                                               ggCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATa                                                                                                       <  3:456858/149‑1 (MQ=255)
                                               ggCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATa                                                                                                       <  4:1593110/149‑1 (MQ=255)
                                               ggCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACg                                                                                                     <  4:1340062/151‑1 (MQ=255)
                                               ggCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACg                                                                                                     <  4:48099/151‑1 (MQ=255)
                                                 cAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGc                                                                                                   >  4:1502693/1‑151 (MQ=255)
                                                   aTTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGccc                                                                                                 >  4:1722631/1‑151 (MQ=255)
                                                     ttCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCgg                                                                                               >  1:477452/1‑151 (MQ=255)
                                                     ttCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCgg                                                                                               >  3:144701/1‑151 (MQ=255)
                                                     ttCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCg                                                                                                <  1:874427/150‑1 (MQ=255)
                                                       ctcGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGtt                                                                                             >  4:1275585/1‑151 (MQ=255)
                                                        tcGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGGTg                                                                                            >  2:167781/1‑151 (MQ=255)
                                                          gcgTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGcc                                                                                          >  3:204196/1‑151 (MQ=255)
                                                             tggtgAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGtt                                                                                       >  2:20911/1‑151 (MQ=255)
                                                                 gAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGatat                                                                                                            >  4:909586/1‑126 (MQ=255)
                                                                 gAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGatat                                                                                                            <  1:909192/126‑1 (MQ=255)
                                                                   aGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGt                                                                                 <  1:1772478/151‑1 (MQ=255)
                                                                    gTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGtg                                                                                <  4:373206/151‑1 (MQ=255)
                                                                          cGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGAc                                                                           >  2:16644/1‑150 (MQ=255)
                                                                             aTTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATg                                                                        >  4:1173894/1‑150 (MQ=255)
                                                                             aTTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATg                                                                        >  4:224413/1‑150 (MQ=255)
                                                                              tttGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGAt                                                                      >  1:352049/1‑151 (MQ=255)
                                                                                 gCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTAc                                                                   <  4:312924/151‑1 (MQ=255)
                                                                                   aaCCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTAccc                                                                 >  4:1913079/1‑151 (MQ=255)
                                                                                    aCCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCg                                                                <  1:315677/151‑1 (MQ=255)
                                                                                       tCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGtt                                                              >  1:614514/1‑150 (MQ=255)
                                                                                        cGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTa                                                             >  4:1382264/1‑150 (MQ=255)
                                                                                        cGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTa                                                             >  1:1555433/1‑150 (MQ=255)
                                                                                         gAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTa                                                             >  1:1202563/1‑149 (MQ=255)
                                                                                          aaGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAg                                                           <  1:1014395/150‑1 (MQ=255)
                                                                                           aGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGa                                                         <  1:153134/151‑1 (MQ=255)
                                                                                               cgcgATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGc                                                       >  1:1407740/1‑149 (MQ=255)
                                                                                               cgcgATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGc                                                       >  2:506550/1‑149 (MQ=255)
                                                                                                   atatCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTg                                                 >  2:115278/1‑151 (MQ=255)
                                                                                                     atCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGAt                                               >  4:1156319/1‑151 (MQ=255)
                                                                                                     atCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGAt                                               <  3:331737/151‑1 (MQ=255)
                                                                                                        gCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCt                                            <  4:486583/151‑1 (MQ=255)
                                                                                                         cccACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTg                                           >  4:701932/1‑151 (MQ=255)
                                                                                                            aCGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGaa                                        <  4:512877/151‑1 (MQ=255)
                                                                                                             cGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAAc                                       >  1:1296894/1‑151 (MQ=255)
                                                                                                                    aGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATggg                                >  4:360032/1‑151 (MQ=255)
                                                                                                                      tCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGc                               >  1:1657307/1‑150 (MQ=255)
                                                                                                                       cGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCa                              <  1:238510/150‑1 (MQ=255)
                                                                                                                          tACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTg                           <  1:1172998/150‑1 (MQ=255)
                                                                                                                             aacaGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCcgc                       >  2:520726/1‑151 (MQ=255)
                                                                                                                             aacaGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCcgc                       <  1:789446/151‑1 (MQ=255)
                                                                                                                               caGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCcgcg                      <  1:1356612/150‑1 (MQ=255)
                                                                                                                                aGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGa                     >  3:194874/1‑150 (MQ=255)
                                                                                                                                 gCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCcgcg                      >  3:38955/1‑148 (MQ=255)
                                                                                                                                   gATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGaagca                 <  1:1574604/151‑1 (MQ=255)
                                                                                                                                    aTGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGaagca                 <  1:1678337/150‑1 (MQ=255)
                                                                                                                                      gCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGaagcaag               <  4:1703879/150‑1 (MQ=255)
                                                                                                                                       cATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGcc             >  1:445552/1‑151 (MQ=255)
                                                                                                                                       cATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGcc             <  4:1369020/151‑1 (MQ=255)
                                                                                                                                        aTTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCt            >  1:1367880/1‑151 (MQ=255)
                                                                                                                                         ttACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTc           >  1:1028497/1‑151 (MQ=255)
                                                                                                                                          tACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCg          >  1:779027/1‑151 (MQ=255)
                                                                                                                                            cGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGt         >  1:1220578/1‑150 (MQ=255)
                                                                                                                                               cGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATaa     <  1:982014/151‑1 (MQ=255)
                                                                                                                                               cGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATaa     <  1:1055666/151‑1 (MQ=255)
                                                                                                                                                gTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATaaa    <  4:264256/151‑1 (MQ=255)
                                                                                                                                                gTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATaa     >  4:751679/1‑150 (MQ=255)
                                                                                                                                                gTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATaa     >  2:298469/1‑150 (MQ=255)
                                                                                                                                                  aTTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAAtt  <  3:390071/151‑1 (MQ=255)
                                                                                                                                                  aTTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAAtt  <  2:100723/151‑1 (MQ=255)
                                                                                                                                                  aTTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAAtt  >  1:1044190/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATT  >  NC_000913/261253‑261549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑