Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,629,930 A→G T47A (ACC→GCC)  yfgH → outer membrane integrity lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,629,9300AG99.3% 543.9 / ‑4.2 144T47A (ACC→GCC) yfgHouter membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (75/68);  total (76/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 146 total aligned reads displayed.

ACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAATTCCGGA  >  NC_000913/2629782‑2630076
                                                                                                                                                    |                                                                                                                                                  
aCAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGcc                                                                                                                                                  >  4:1928872/1‑151 (MQ=255)
aCAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGcc                                                                                                                                                  >  2:267264/1‑151 (MQ=255)
  aaGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGt                                                                                                                                                <  1:1612568/151‑1 (MQ=255)
   aGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGtt                                                                                                                                               >  1:1133064/1‑151 (MQ=255)
    ggAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTa                                                                                                                                              <  1:1879985/151‑1 (MQ=255)
      aaTAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAat                                                                                                                                            <  4:1911383/151‑1 (MQ=255)
        tAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAatat                                                                                                                                          >  1:1057546/1‑151 (MQ=255)
        tAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAata                                                                                                                                           <  1:1171531/150‑1 (MQ=255)
              tgaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTc                                                                                                                                    >  4:1633861/1‑151 (MQ=255)
               gaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTc                                                                                                                                    <  4:1230811/150‑1 (MQ=255)
                aaaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTcc                                                                                                                                   >  2:257926/1‑150 (MQ=255)
                aaaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTcc                                                                                                                                   >  4:1646554/1‑150 (MQ=255)
                aaaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTcc                                                                                                                                   >  1:1541447/1‑150 (MQ=255)
                aaaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCa                                                                                                                                  <  1:366235/151‑1 (MQ=255)
                 aaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCAt                                                                                                                                 <  1:1339635/151‑1 (MQ=255)
                 aaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCAt                                                                                                                                 <  4:1292107/151‑1 (MQ=255)
                    ttAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCAttct                                                                                                                              >  1:1699618/1‑151 (MQ=255)
                     tAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCAttctt                                                                                                                             >  2:205350/1‑151 (MQ=255)
                      aaaaaaTGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCAttctt                                                                                                                             <  1:1292077/150‑1 (MQ=255)
                        aaaaTGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTccc                                                                                                                          >  1:271883/1‑151 (MQ=255)
                         aaaTGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTccca                                                                                                                         <  3:321982/151‑2 (MQ=255)
                         aaaTGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCg                                                                                                                         >  1:1442011/1‑151 (MQ=255)
                             gTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCaa                                                                                                                      <  4:779552/150‑1 (MQ=255)
                                 tctGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCaaaa                                                                                                                    <  4:423949/148‑1 (MQ=255)
                                   tGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGc                                                                                                               >  4:289265/1‑151 (MQ=255)
                                   tGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGc                                                                                                               >  4:544341/1‑151 (MQ=255)
                                    gCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGtt                                                                                                                 <  1:901000/148‑1 (MQ=255)
                                    gCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGtt                                                                                                                 <  4:191947/148‑1 (MQ=255)
                                    gCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGcc                                                                                                              >  1:1893000/1‑151 (MQ=255)
                                     ccTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAACTGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGCTGCCg                                                                                                             >  1:1767014/1‑151 (MQ=255)
                                     ccTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGcc                                                                                                              >  4:1131849/1‑150 (MQ=255)
                                     ccTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCg                                                                                                             >  4:1194724/1‑151 (MQ=255)
                                          ggCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGAc                                                                                                        <  1:968388/151‑1 (MQ=255)
                                           gCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACa                                                                                                       <  4:1084011/151‑1 (MQ=255)
                                           gCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACa                                                                                                       <  1:1759225/151‑1 (MQ=255)
                                             aaTGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAAc                                                                                                     >  4:89774/1‑151 (MQ=255)
                                               tGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTc                                                                                                   >  4:492245/1‑151 (MQ=255)
                                                 ttAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTccc                                                                                                 >  4:874991/1‑151 (MQ=255)
                                                  tAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTccc                                                                                                 <  1:215445/150‑1 (MQ=255)
                                                   aGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCaa                                                                                               <  1:122583/151‑1 (MQ=255)
                                                    gCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCaaa                                                                                              <  3:164431/151‑1 (MQ=255)
                                                        cATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATaa                                                                                          <  1:221069/151‑1 (MQ=255)
                                                        cATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATa                                                                                           <  2:358346/150‑1 (MQ=255)
                                                           tCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAAc                                                                                        <  3:213950/150‑1 (MQ=255)
                                                           tCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACg                                                                                       >  1:971666/1‑151 (MQ=255)
                                                             aCTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACgg                                                                                      >  4:1302822/1‑150 (MQ=255)
                                                               tctGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAAc                                                                                   >  4:1681453/1‑151 (MQ=255)
                                                                ctGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGaa                                                                                    <  1:1084646/149‑1 (MQ=255)
                                                                  ggCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACg                                                                                  <  1:406465/149‑1 (MQ=255)
                                                                  ggCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGca                                                                                <  4:317935/151‑1 (MQ=255)
                                                                    cAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGcac                                                                               >  3:506044/1‑150 (MQ=255)
                                                                      ggATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAg                                                                            <  1:1514895/151‑1 (MQ=255)
                                                                          gCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCtt                                                                        >  1:1111810/1‑151 (MQ=255)
                                                                          gCCAGACAAATGCTGACGATCATGCTGCCGATGGTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAATCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGcaca                                                                              >  3:381212/1‑145 (MQ=255)
                                                                           ccAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTc                                                                       <  4:1001104/151‑1 (MQ=255)
                                                                           ccAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTc                                                                       >  3:117377/1‑151 (MQ=255)
                                                                             aGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCgg                                                                     <  1:72351/151‑1 (MQ=255)
                                                                                 aaaTGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGgcgc                                                                  >  1:584482/1‑150 (MQ=255)
                                                                                   aTGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCt                                                               >  1:44998/1‑151 (MQ=255)
                                                                                     gCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCtt                                                              <  1:489186/150‑1 (MQ=255)
                                                                                     gCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTa                                                             <  1:1260395/151‑1 (MQ=255)
                                                                                       tGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTAtt                                                           >  2:287355/1‑151 (MQ=255)
                                                                                          cGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGgc                                                        <  1:1001968/151‑1 (MQ=255)
                                                                                           gATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGgcg                                                       >  1:72659/1‑151 (MQ=255)
                                                                                              cATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAg                                                    <  1:1928133/151‑1 (MQ=255)
                                                                                                 gctgcCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCg                                                 <  3:180813/151‑1 (MQ=255)
                                                                                                 gctgcCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCg                                                 <  1:482145/151‑1 (MQ=255)
                                                                                                  ctgcCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCg                                                 <  4:1454218/150‑1 (MQ=255)
                                                                                                  ctgcCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGc                                                >  2:217496/1‑151 (MQ=255)
                                                                                                   tgcCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCt                                               >  2:474770/1‑151 (MQ=255)
                                                                                                    gcCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTg                                              <  1:181331/151‑1 (MQ=255)
                                                                                                     ccGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTgg                                             >  4:660875/1‑151 (MQ=255)
                                                                                                      cGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGc                                            >  2:364193/1‑151 (MQ=255)
                                                                                                      cGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGc                                            <  1:1799627/151‑1 (MQ=255)
                                                                                                      cGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGc                                            <  4:1767707/151‑1 (MQ=255)
                                                                                                       gATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCg                                           <  1:1370015/151‑1 (MQ=255)
                                                                                                        aTGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCgg                                          >  4:956374/1‑151 (MQ=255)
                                                                                                          gTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGgtg                                        >  3:395882/1‑151 (MQ=255)
                                                                                                           tttATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGgtg                                        >  4:363821/1‑150 (MQ=255)
                                                                                                               tCAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGGGTTATc                                   >  1:157864/1‑151 (MQ=255)
                                                                                                                cAAACCGATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATc                                   <  1:1869407/150‑1 (MQ=255)
                                                                                                                      gATCAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCcaca                            <  1:1565408/151‑1 (MQ=255)
                                                                                                                         cAACTGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAAc                          >  2:420342/1‑150 (MQ=255)
                                                                                                                             tGAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCgg                     >  4:95550/1‑151 (MQ=255)
                                                                                                                              gAATACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTTGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCggg                    >  4:1681247/1‑151 (MQ=255)
                                                                                                                               aaTACCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGt                   <  3:72265/151‑1 (MQ=255)
                                                                                                                                  aCCAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCt                 <  2:491821/150‑1 (MQ=255)
                                                                                                                                    cAAACAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTgg               >  1:331269/1‑150 (MQ=255)
                                                                                                                                     aaaCAAGAACCTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCt                 >  2:394351/1‑147 (MQ=255)
                                                                                                                                       aCAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTgg               >  4:1462573/1‑147 (MQ=255)
                                                                                                                                        cAAGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGgcagc           >  4:1206046/1‑150 (MQ=255)
                                                                                                                                         aaGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGgcagca          >  1:1927972/1‑150 (MQ=255)
                                                                                                                                         aaGAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGgcagca          >  1:628738/1‑150 (MQ=255)
                                                                                                                                           gAAACTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGGTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAAtt       >  1:803420/1‑151 (MQ=255)
                                                                                                                                            aaaCTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAATTc      <  3:221157/151‑1 (MQ=255)
                                                                                                                                            aaaCTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAATTc      >  4:1783459/1‑151 (MQ=255)
                                                                                                                                             aaCTAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAATTcc     <  1:520175/151‑1 (MQ=255)
                                                                                                                                                tAAAGCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAATTCCgg   >  1:847440/1‑150 (MQ=255)
                                                                                                                                                 aaagCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACATCGTCGGGTCTGGCAGCAATTCCgg   <  1:890643/149‑1 (MQ=255)
                                                                                                                                                 aaagCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAATTCCGGa  >  1:572639/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGTCGGGTCTGGCAGCAATTCCGGA  >  NC_000913/2629782‑2630076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑