Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,647,150 G→A A59V (GCT→GTT)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,647,1500GA100.0% 501.0 / NA 123A59V (GCT→GTT) pbpCpenicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (71/52);  total (71/52)

Only 100 of 123 total aligned reads displayed.

AGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAG  >  NC_000913/2647002‑2647296
                                                                                                                                                    |                                                                                                                                                  
aGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACa                                                                                                                                                  <  1:455857/151‑1 (MQ=255)
    agaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCgg                                                                                                                                              >  4:337164/1‑151 (MQ=255)
    agaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCgg                                                                                                                                              <  4:1428373/151‑1 (MQ=255)
     gaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGc                                                                                                                                             >  1:1584732/1‑151 (MQ=255)
          tGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCg                                                                                                                                            <  1:555065/147‑1 (MQ=255)
          tGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGaa                                                                                                                                          <  4:1381194/149‑1 (MQ=255)
          tGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAg                                                                                                                                         <  1:1893493/150‑1 (MQ=255)
              aTGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGcc                                                                                                                                     >  1:764133/1‑150 (MQ=255)
                gctgcGCGCACCACCGAGAATTGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCag                                                                                                                                   <  1:1437870/150‑1 (MQ=255)
                 ctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCag                                                                                                                                   >  2:234931/1‑149 (MQ=255)
                     gcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAgcggc                                                                                                                             >  4:574596/1‑151 (MQ=255)
                        cgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGt                                                                                                                           >  1:1194735/1‑150 (MQ=255)
                           cACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTAcc                                                                                                                        >  1:608079/1‑150 (MQ=255)
                             ccGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCAt                                                                                                                      <  4:651772/150‑1 (MQ=255)
                             ccGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCAt                                                                                                                      >  4:419699/1‑150 (MQ=255)
                                agaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTg                                                                                                                  >  4:331680/1‑151 (MQ=255)
                                agaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTg                                                                                                                  <  1:1130004/151‑1 (MQ=255)
                                 gaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTgc                                                                                                                 >  1:48194/1‑151 (MQ=255)
                                       aTTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACg                                                                                                           <  4:1483455/151‑1 (MQ=255)
                                         tCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGa                                                                                                          <  1:1017155/150‑1 (MQ=255)
                                           aCCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGAcc                                                                                                        >  4:1677157/1‑150 (MQ=255)
                                            cccccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCAc                                                                                                      >  1:1531516/1‑151 (MQ=255)
                                             ccccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACt                                                                                                     >  2:454253/1‑151 (MQ=255)
                                                cGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGt                                                                                                  >  2:259345/1‑151 (MQ=255)
                                                 ggATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTg                                                                                                 >  4:1605427/1‑151 (MQ=255)
                                                 ggATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTg                                                                                                 >  4:636104/1‑151 (MQ=255)
                                                   aTGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCg                                                                                               >  4:1449611/1‑151 (MQ=255)
                                                   aTGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGACAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCg                                                                                               <  3:542792/151‑1 (MQ=255)
                                                     gCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCggg                                                                                             >  2:38991/1‑151 (MQ=255)
                                                     gCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCgg                                                                                              <  1:1098077/150‑1 (MQ=255)
                                                         ccAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTg                                                                                         >  1:1772794/1‑151 (MQ=255)
                                                           aGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGAc                                                                                       >  4:971243/1‑151 (MQ=255)
                                                            gAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt                                                                                      >  1:1653669/1‑151 (MQ=255)
                                                            gAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACt                                                                                      >  4:37440/1‑151 (MQ=255)
                                                              aCCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTc                                                                                    <  4:466520/151‑1 (MQ=255)
                                                                 aGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATg                                                                                 <  2:496451/151‑1 (MQ=255)
                                                                 aGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATg                                                                                 <  2:336269/151‑1 (MQ=255)
                                                                     aTCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGc                                                                             >  4:1580839/1‑151 (MQ=255)
                                                                        ttCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGt                                                                          >  1:1919690/1‑151 (MQ=255)
                                                                         tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCa                                                                               <  1:1385188/145‑1 (MQ=255)
                                                                         tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGc                                                                             <  4:1297997/147‑1 (MQ=255)
                                                                         tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg                                                                           <  1:536432/149‑1 (MQ=255)
                                                                         tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg                                                                           <  4:321554/149‑1 (MQ=255)
                                                                          cATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGt                                                                          >  4:1066325/1‑149 (MQ=255)
                                                                           aTAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTaga                                                                       <  1:1763376/151‑1 (MQ=255)
                                                                             aaTTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAgg                                                                     <  1:99476/151‑1 (MQ=255)
                                                                              aTTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGc                                                                    >  4:696070/1‑151 (MQ=255)
                                                                                 gATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                   >  4:1873745/1‑149 (MQ=255)
                                                                                  aTCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                   >  2:116571/1‑148 (MQ=255)
                                                                                  aTCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                   >  4:1005606/1‑148 (MQ=255)
                                                                                  aTCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                   >  2:496159/1‑148 (MQ=255)
                                                                                  aTCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCaga                                                                <  1:358342/151‑1 (MQ=255)
                                                                                   tCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCaga                                                                <  2:41118/150‑1 (MQ=255)
                                                                                     aGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGtt                                                             >  4:524974/1‑151 (MQ=255)
                                                                                     aGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGtt                                                             <  1:940373/151‑1 (MQ=255)
                                                                                      gcgcTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGttt                                                            >  2:40595/1‑151 (MQ=255)
                                                                                        gcTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTAt                                                          >  4:1394196/1‑151 (MQ=255)
                                                                                          ttCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCt                                                        >  3:415142/1‑151 (MQ=255)
                                                                                           tCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCt                                                        <  1:1818580/150‑1 (MQ=255)
                                                                                            cAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGc                                                      >  1:201507/1‑151 (MQ=255)
                                                                                             aaGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGCAGCGCCAGTGCGGCGTACCCTCCCGCGCCACGACCACTTGTGCGCGATTGACTTCATGAAGCGGCACGGGCCAGAGTTTATCTGCt                                                     >  1:1578761/1‑151 (MQ=255)
                                                                                             aaGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCt                                                     <  4:597638/151‑1 (MQ=255)
                                                                                              aGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTc                                                    >  1:209666/1‑151 (MQ=255)
                                                                                              aGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTc                                                    >  2:545282/1‑151 (MQ=255)
                                                                                                  aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTcccc                                                 >  1:830027/1‑150 (MQ=255)
                                                                                                  aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTcccc                                                 <  4:1352381/150‑1 (MQ=255)
                                                                                                  aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTcccc                                                 <  1:1436639/150‑1 (MQ=255)
                                                                                                   aCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCAc                                               >  3:490827/1‑151 (MQ=255)
                                                                                                   aCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGGAGAGGCCAGAGTTTATCTGCTCCCCa                                                >  3:178265/1‑150 (MQ=255)
                                                                                                       ggAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTg                                           >  4:219031/1‑151 (MQ=255)
                                                                                                       ggAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTg                                           <  4:1289184/151‑1 (MQ=255)
                                                                                                         agaAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCa                                         <  1:1921470/151‑1 (MQ=255)
                                                                                                          gaAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCa                                         >  1:1120496/1‑150 (MQ=255)
                                                                                                           aaaCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCa                                         >  3:184749/1‑149 (MQ=255)
                                                                                                            aaCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGa                                      <  4:546862/151‑1 (MQ=255)
                                                                                                             aCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGa                                      >  2:62531/1‑150 (MQ=255)
                                                                                                                   tCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                                <  1:1400179/150‑1 (MQ=255)
                                                                                                                    cGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                                >  1:964624/1‑149 (MQ=255)
                                                                                                                    cGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAg                                 <  4:1856300/148‑1 (MQ=255)
                                                                                                                    cGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGgag                              <  4:1826683/151‑1 (MQ=255)
                                                                                                                      aTTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGaa                            <  4:1008284/151‑1 (MQ=255)
                                                                                                                         gTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAgg                         >  1:1800783/1‑151 (MQ=255)
                                                                                                                         gTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAg                          <  4:1139944/150‑1 (MQ=255)
                                                                                                                           tACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgc                       >  2:229764/1‑151 (MQ=255)
                                                                                                                            aCCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgcg                      <  1:1037859/151‑1 (MQ=255)
                                                                                                                             ccGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgcgc                     >  1:1302453/1‑151 (MQ=255)
                                                                                                                              cGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGcgcg                    >  2:464457/1‑151 (MQ=255)
                                                                                                                                gATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGc                  >  4:587809/1‑151 (MQ=255)
                                                                                                                                 aTAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCt                 >  1:1042447/1‑151 (MQ=255)
                                                                                                                                  tAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTg                >  1:944773/1‑151 (MQ=255)
                                                                                                                                  tAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTg                >  1:1191485/1‑151 (MQ=255)
                                                                                                                                   aaCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGc               <  4:1300636/151‑1 (MQ=255)
                                                                                                                                   aaCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGc               >  4:1298909/1‑151 (MQ=255)
                                                                                                                                   aaCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGc               >  2:392594/1‑151 (MQ=255)
                                                                                                                                    aCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGcc              <  3:327836/151‑1 (MQ=255)
                                                                                                                                    aCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGcc              <  1:1033592/151‑1 (MQ=255)
                                                                                                                                       ccAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAAc           >  4:606650/1‑151 (MQ=255)
                                                                                                                                             gCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTAt      <  3:267882/150‑1 (MQ=255)
                                                                                                                                               cGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATcc    >  1:739989/1‑150 (MQ=255)
                                                                                                                                                gTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCcag  <  4:1369984/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAG  >  NC_000913/2647002‑2647296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑