Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,662,523 A→G intergenic (‑392/+60) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,662,5230AG98.8% 535.4 / NA 165intergenic (‑392/+60)iscR/trmJisc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (56/106);  minor base T (2/0);  total (59/106)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 165 total aligned reads displayed.

TACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCA  >  NC_000913/2662376‑2662670
                                                                                                                                                   |                                                                                                                                                   
tACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCgg                                                                                                                                                  >  4:930388/1‑151 (MQ=255)
tACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCg                                                                                                                                                   <  4:532652/150‑1 (MQ=255)
     gTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCCGCGGTTCTg                                                                                                                                             >  2:237144/1‑151 (MQ=255)
      tcggagtaGGCACCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCt                                                                                                                                              <  3:463329/142‑1 (MQ=14)
       cGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGtt                                                                                                                                           <  4:1529976/151‑1 (MQ=255)
       cGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGtt                                                                                                                                           <  4:1483561/151‑1 (MQ=255)
         cATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTc                                                                                                                                          <  4:129835/150‑1 (MQ=255)
            cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTg                                                                                                                                      <  4:704564/151‑1 (MQ=255)
            cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTg                                                                                                                                      <  4:1716505/151‑1 (MQ=255)
               gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAg                                                                                                                                   <  1:1385145/151‑1 (MQ=255)
                       tGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGGGTGAACCCCTTTTCCGCCCCGCGGTTCTGGTCCCCATAAGCCTGa                                                                                                                             >  1:1544051/1‑149 (MQ=14)
                        gTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCCGATa                                                                                                                           >  4:1073161/1‑150 (MQ=255)
                          cGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAg                                                                                                                         <  1:443424/150‑1 (MQ=25)
                          cGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGa                                                                                                                        <  4:838638/151‑1 (MQ=255)
                           ggATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGTACCTCTTTTCCGATCTTCGGTTCTGGTCACTGTACGCCTGAGaa                                                                                                                          >  1:771048/1‑148 (MQ=14)
                           ggATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGAc                                                                                                                       <  4:163327/151‑1 (MQ=255)
                           ggATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGGTCACTGTAGGCCTGATAAGa                                                                                                                        >  4:1904780/1‑150 (MQ=255)
                            gATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACg                                                                                                                      <  4:285636/151‑1 (MQ=255)
                               gcggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCAt                                                                                                                   <  4:1172306/151‑1 (MQ=255)
                                cggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCAtt                                                                                                                  >  4:1129035/1‑151 (MQ=255)
                                cggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCAtt                                                                                                                  <  1:86910/151‑1 (MQ=255)
                                 ggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTa                                                                                                                 <  1:807117/151‑1 (MQ=255)
                                      gAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcgcg                                                                                                             >  4:1783780/1‑150 (MQ=255)
                                              tATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCa                                                                                                     <  1:1119997/150‑1 (MQ=38)
                                              tATCCGACCTACGGTTCTGTTCACTGTAGGCCTAATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATc                                                                                                      <  4:1601425/149‑1 (MQ=255)
                                               aTCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                                                                                   <  1:1050507/151‑1 (MQ=38)
                                                  cGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCaa                                                                                                <  4:340520/151‑1 (MQ=37)
                                                  cGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCa                                                                                                 >  4:327326/1‑150 (MQ=38)
                                                   gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                                                                               <  2:494660/151‑1 (MQ=37)
                                                     ccTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                                                                             <  1:1496828/151‑1 (MQ=25)
                                                     ccTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACg                                                                                              <  1:1072763/150‑1 (MQ=25)
                                                     ccTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGAGAAGACGCCATACGCGTCGCATCAGGCAACgg                                                                                             >  4:872922/1‑151 (MQ=255)
                                                       tACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCt                                                                                           >  4:1006967/1‑151 (MQ=18)
                                                       tACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACCCGTCGCATCAGGCAACGGc                                                                                            >  1:1536822/1‑150 (MQ=18)
                                                        aCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTg                                                                                          <  4:892580/151‑1 (MQ=18)
                                                         cGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGt                                                                                         <  4:785552/151‑1 (MQ=17)
                                                          ggTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTggt                                                                                        >  1:1220540/1‑149 (MQ=14)
                                                            ttCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTAt                                                                                      >  4:1850644/1‑151 (MQ=18)
                                                               tGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCg                                                                                   >  4:1577615/1‑151 (MQ=25)
                                                                gTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTc                                                                                    <  1:593059/149‑1 (MQ=25)
                                                                gTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCgg                                                                                  <  4:398073/151‑1 (MQ=37)
                                                                gTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCgg                                                                                  >  1:698286/1‑151 (MQ=37)
                                                                 ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTggg                                                                                  <  1:1376306/150‑1 (MQ=18)
                                                                  tCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGcc                                                                                >  1:217329/1‑151 (MQ=37)
                                                                   cACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGcc                                                                                >  1:181150/1‑150 (MQ=25)
                                                                   cACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGGCGCATCAGGCAACGGCTGTTATTCGGcc                                                                                >  1:270781/1‑150 (MQ=25)
                                                                    aCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCtt                                                                              <  3:494307/151‑1 (MQ=37)
                                                                    aCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCtt                                                                              <  1:1012593/151‑1 (MQ=37)
                                                                       gTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGt                                                                            <  4:1037731/150‑1 (MQ=255)
                                                                         aGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTa                                                                          <  1:488921/150‑1 (MQ=255)
                                                                         aGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTa                                                                          <  2:35699/150‑1 (MQ=255)
                                                                         aGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTAc                                                                         <  2:351399/151‑1 (MQ=255)
                                                                           gCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTAcc                                                                        >  3:20075/1‑150 (MQ=255)
                                                                           gCCTGATAAGACGCATTACGCGGCGCATCAGGCATCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTa                                                                          <  3:34600/148‑1 (MQ=255)
                                                                             cTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCttt                                                                     >  4:623140/1‑151 (MQ=255)
                                                                               gATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTa                                                                    <  2:365066/150‑1 (MQ=255)
                                                                               gATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAt                                                                   <  4:34799/151‑1 (MQ=255)
                                                                                 tAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAtt                                                                  <  1:1319057/150‑1 (MQ=255)
                                                                                  aaGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTct                                                                <  4:91785/151‑1 (MQ=255)
                                                                                  aaGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTct                                                                <  1:728179/151‑1 (MQ=255)
                                                                                  aaGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTct                                                                <  2:203092/151‑1 (MQ=255)
                                                                                   aGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctg                                                               <  4:473588/151‑1 (MQ=255)
                                                                                         aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTc                                                         <  1:1260450/151‑1 (MQ=255)
                                                                                         aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTc                                                         <  1:1351479/151‑1 (MQ=255)
                                                                                           tACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCa                                                        <  1:280491/150‑1 (MQ=255)
                                                                                              gcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAg                                                    <  2:17823/151‑1 (MQ=255)
                                                                                               cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGa                                                   <  4:1924270/151‑1 (MQ=255)
                                                                                               cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGa                                                   <  4:1330226/151‑1 (MQ=255)
                                                                                                gTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGa                                                   >  4:1442402/1‑150 (MQ=255)
                                                                                                  cGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGc                                                <  1:840265/151‑1 (MQ=255)
                                                                                                    cATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa                                              <  1:929987/151‑1 (MQ=255)
                                                                                                     aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAg                                             <  1:1323986/151‑1 (MQ=255)
                                                                                                       cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGa                                            <  1:537471/150‑1 (MQ=255)
                                                                                                       cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGa                                            <  1:1738431/150‑1 (MQ=255)
                                                                                                          gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATcc                                        <  4:1282542/151‑1 (MQ=255)
                                                                                                           cAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAAAccc                                       >  1:536294/1‑151 (MQ=255)
                                                                                                            aaCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATccc                                       >  3:269155/1‑150 (MQ=255)
                                                                                                            aaCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcg                                      <  1:977762/151‑1 (MQ=255)
                                                                                                             aCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcgc                                     >  4:619929/1‑151 (MQ=255)
                                                                                                              cGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAGGCCAGAATCCcgc                                     <  1:1641545/150‑1 (MQ=255)
                                                                                                                   gTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAgg                                <  2:62995/150‑1 (MQ=255)
                                                                                                                     cGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGata                             <  1:1675905/151‑1 (MQ=255)
                                                                                                                       gATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATAtt                           <  1:1139880/151‑1 (MQ=255)
                                                                                                                          gcggcgTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCa                         <  1:759499/150‑1 (MQ=255)
                                                                                                                           cggcgTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa                        >  1:1512995/1‑150 (MQ=255)
                                                                                                                            ggcgTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACt                      >  1:891764/1‑151 (MQ=255)
                                                                                                                               gTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCt                   <  1:1177937/151‑1 (MQ=255)
                                                                                                                                tGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTg                  >  1:1051594/1‑151 (MQ=255)
                                                                                                                                   aCGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCt               <  2:417314/151‑1 (MQ=255)
                                                                                                                                   aCGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCt               <  1:1416065/151‑1 (MQ=255)
                                                                                                                                     gCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt             >  4:1655323/1‑151 (MQ=255)
                                                                                                                                         tATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCgg         >  4:22844/1‑151 (MQ=255)
                                                                                                                                          aTCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTATCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCggg        >  1:1013915/1‑151 (MQ=255)
                                                                                                                                          aTCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCgg         <  1:1496269/150‑1 (MQ=255)
                                                                                                                                            ccGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcg      <  1:621577/151‑1 (MQ=255)
                                                                                                                                             cGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgc     <  1:1849924/151‑1 (MQ=255)
                                                                                                                                             cGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgc     <  1:1275679/151‑1 (MQ=255)
                                                                                                                                              gACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcg    <  4:969224/151‑1 (MQ=255)
                                                                                                                                               aCCTGCGGTTCTCTTCACTGTATGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGACTTGGTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCCCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcgc   >  1:371981/1‑151 (MQ=37)
                                                                                                                                                ccTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCa  <  1:43158/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCA  >  NC_000913/2662376‑2662670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: