Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,706,291 A→G R278R (CGT→CGC)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,706,2910AG100.0% 497.9 / NA 133R278R (CGT→CGC) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (65/68);  total (65/68)

Only 100 of 134 total aligned reads displayed.

CATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTACGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGC  >  NC_000913/2706146‑2706438
                                                                                                                                                 |                                                                                                                                                   
cATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTgcgc                                                                                                                                                  <  2:538531/149‑1 (MQ=255)
    gAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGc                                                                                                                                            >  3:523407/1‑151 (MQ=255)
        aGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGtt                                                                                                                                         >  1:1701338/1‑150 (MQ=255)
        aGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGtt                                                                                                                                         >  1:1305088/1‑150 (MQ=255)
        aGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTa                                                                                                                                        <  1:1722434/151‑1 (MQ=255)
        aGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTa                                                                                                                                        <  1:1127400/151‑1 (MQ=255)
        aGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTa                                                                                                                                        <  3:106316/151‑1 (MQ=255)
           gAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAg                                                                                                                                      <  1:842578/150‑1 (MQ=255)
            aGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGt                                                                                                                                    <  4:265835/151‑1 (MQ=255)
             ggCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGt                                                                                                                                    >  1:295121/1‑150 (MQ=255)
              gCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCTCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTAt                                                                                                                                  <  2:297535/151‑1 (MQ=255)
                aTCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCg                                                                                                                                >  1:1194298/1‑151 (MQ=255)
                aTCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCg                                                                                                                                <  4:448246/151‑1 (MQ=255)
                    ttCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCg                                                                                                                             >  3:100187/1‑150 (MQ=255)
                    ttCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCg                                                                                                                             >  1:513347/1‑150 (MQ=255)
                     tCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGa                                                                                                                            <  3:546035/150‑1 (MQ=255)
                      cAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGAc                                                                                                                           <  1:967088/150‑1 (MQ=255)
                      cAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACt                                                                                                                          >  4:1314496/1‑151 (MQ=255)
                         gCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGa                                                                                                                       <  4:98056/151‑1 (MQ=255)
                            gAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAgcg                                                                                                                    <  1:1652237/151‑1 (MQ=255)
                                   cccAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGa                                                                                                             <  4:539850/151‑1 (MQ=255)
                                     cAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGat                                                                                                            >  1:212858/1‑150 (MQ=255)
                                       gcgcGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGGTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGatat                                                                                                          >  3:185435/1‑150 (MQ=255)
                                        cgcgTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCt                                                                                                        <  3:92234/151‑1 (MQ=255)
                                        cgcgTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCt                                                                                                        >  2:507442/1‑151 (MQ=255)
                                         gcgTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCtt                                                                                                       <  1:1338315/151‑1 (MQ=255)
                                           gTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTa                                                                                                     <  4:80324/151‑1 (MQ=255)
                                              aCGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATc                                                                                                  <  4:1471860/151‑1 (MQ=255)
                                              aCGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATc                                                                                                  >  4:1855831/1‑151 (MQ=255)
                                               cGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCg                                                                                                 >  4:1366163/1‑151 (MQ=255)
                                               cGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCg                                                                                                 <  4:925662/151‑1 (MQ=255)
                                               cGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCg                                                                                                 <  1:1077856/151‑1 (MQ=255)
                                                 gAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATGCCTGCGGTTTGACTTTCTGAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACGGGAGCACCGTAGATATCTTTAATCGca                                                                                               <  1:1781761/151‑1 (MQ=255)
                                                 gAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGca                                                                                               <  1:836528/151‑1 (MQ=255)
                                                 gAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGc                                                                                                >  1:488769/1‑150 (MQ=255)
                                                    ggCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACAt                                                                                            <  3:251791/151‑1 (MQ=255)
                                                     gCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACAt                                                                                            >  2:438316/1‑150 (MQ=255)
                                                        tCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACg                                                                                         <  4:633330/150‑1 (MQ=255)
                                                            aGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGagcc                                                                                     >  1:1388592/1‑150 (MQ=255)
                                                               cgtcgGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGagccagc                                                                                  <  4:619977/150‑1 (MQ=255)
                                                                 tcgGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCt                                                                                >  4:1764104/1‑150 (MQ=255)
                                                                  cgGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTAc                                                                              >  1:990367/1‑151 (MQ=255)
                                                                     aaCTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAACGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTc                                                                           >  4:1667456/1‑151 (MQ=255)
                                                                      aCTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCg                                                                          <  1:1561828/151‑1 (MQ=255)
                                                                       cTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGc                                                                         >  2:269890/1‑151 (MQ=255)
                                                                       cTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGc                                                                         >  3:211978/1‑151 (MQ=255)
                                                                       cTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGc                                                                         >  2:472846/1‑151 (MQ=255)
                                                                        ttACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGcc                                                                        >  1:372194/1‑151 (MQ=255)
                                                                             gggAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACAtt                                                                   >  1:1703169/1‑151 (MQ=255)
                                                                                aaCAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATttca                                                                <  1:1814971/151‑1 (MQ=255)
                                                                                aaCAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATttc                                                                 >  1:1152602/1‑150 (MQ=255)
                                                                                     aCCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATttcagttca                                                           <  1:20590/151‑1 (MQ=255)
                                                                                     aCCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATttcagttc                                                            >  1:1897579/1‑150 (MQ=255)
                                                                                          cGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGc                                                       <  4:409706/150‑1 (MQ=255)
                                                                                          cGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCg                                                      <  4:174363/151‑1 (MQ=255)
                                                                                           gTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCgg                                                     >  1:566618/1‑151 (MQ=255)
                                                                                             ataCCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTc                                                   <  1:1607722/151‑1 (MQ=255)
                                                                                               aCCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCa                                                  >  4:1913344/1‑150 (MQ=255)
                                                                                               aCCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCa                                                  >  1:1483449/1‑150 (MQ=255)
                                                                                               aCCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCa                                                  >  4:417292/1‑150 (MQ=255)
                                                                                                ccTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCaa                                                 <  4:1845967/150‑1 (MQ=255)
                                                                                                ccTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCaa                                                 <  1:946066/150‑1 (MQ=255)
                                                                                                     ggTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGtt                                           <  1:1653985/151‑1 (MQ=255)
                                                                                                     ggTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGtt                                           <  1:301868/151‑1 (MQ=255)
                                                                                                     ggTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGtt                                           <  4:683606/151‑1 (MQ=255)
                                                                                                     ggTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGtt                                           >  2:360048/1‑151 (MQ=255)
                                                                                                      gTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCTGTGGATATCTTTAATCCCACATACGGGCCAGCCTTCATCGCCACATTTCAGTGCAGTGCGGTCAACCTGttt                                          <  1:1047509/151‑1 (MQ=255)
                                                                                                          gACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCgg                                       <  3:261139/150‑1 (MQ=255)
                                                                                                            cTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGt                                    >  3:227663/1‑151 (MQ=255)
                                                                                                              ttCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGt                                    <  4:513895/149‑1 (MQ=255)
                                                                                                                cTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTg                                   >  4:1173942/1‑148 (MQ=255)
                                                                                                                cTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGa                                  >  4:608479/1‑149 (MQ=255)
                                                                                                                  tAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGTCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGa                               <  1:1756414/150‑1 (MQ=255)
                                                                                                                  tAAAGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGa                               <  4:1493545/150‑1 (MQ=255)
                                                                                                                   aaaGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGAt                              <  3:39307/150‑1 (MQ=255)
                                                                                                                     aGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGcc                           >  1:582932/1‑151 (MQ=255)
                                                                                                                     aGCCAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGcc                           >  1:1540784/1‑151 (MQ=255)
                                                                                                                       ccAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGccc                          >  4:664302/1‑150 (MQ=255)
                                                                                                                        cAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGccc                          >  4:1025598/1‑149 (MQ=255)
                                                                                                                        cAGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGccc                          >  1:657112/1‑149 (MQ=255)
                                                                                                                         aGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGccc                          >  3:448432/1‑148 (MQ=255)
                                                                                                                         aGGCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGccc                          >  3:370469/1‑148 (MQ=255)
                                                                                                                           gCAGCGCCTTTTCTGCCGGATTGCGTGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACg                     <  3:204657/151‑1 (MQ=255)
                                                                                                                           gCAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACg                     <  4:815744/151‑1 (MQ=255)
                                                                                                                            cAGCGCCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACgg                    <  2:519466/151‑1 (MQ=255)
                                                                                                                              gcgcCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTc                  >  2:464277/1‑151 (MQ=255)
                                                                                                                                gcCTTTTCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCgg                <  4:513552/151‑1 (MQ=255)
                                                                                                                                   ttttCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGt             >  3:481641/1‑151 (MQ=255)
                                                                                                                                    tttCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGt             <  4:854236/150‑1 (MQ=255)
                                                                                                                                     ttCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGtt            >  3:86240/1‑150 (MQ=255)
                                                                                                                                      tCTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTtat          >  2:242285/1‑151 (MQ=255)
                                                                                                                                       cTGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTtat          >  1:1111227/1‑150 (MQ=255)
                                                                                                                                        tGCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTtat          >  1:867541/1‑149 (MQ=255)
                                                                                                                                         gCCGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTtata         <  2:413162/149‑1 (MQ=255)
                                                                                                                                           cGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTc     >  4:722671/1‑151 (MQ=255)
                                                                                                                                           cGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTc     >  4:1205348/1‑151 (MQ=255)
                                                                                                                                           cGGATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCCTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTc     <  4:1305628/151‑1 (MQ=255)
                                                                                                                                            ggATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTc     >  1:539944/1‑150 (MQ=255)
                                                                                                                                            ggATTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCt    >  4:124104/1‑151 (MQ=255)
                                                                                                                                              aTTGCGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGc  <  2:153025/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCTGCCGGATTACGCGCCAGCGTTAAGGTATCGCCGACTGGAGCGCCGTGGATATCTTTAATCGCACATACGAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGC  >  NC_000913/2706146‑2706438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑