Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,780,330 G→A G799G (GGC→GGT ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,780,3300GA99.2% 510.9 / NA 131G799G (GGC→GGTypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (61/69);  minor base T (0/1);  total (61/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 131 total aligned reads displayed.

TTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCC  >  NC_000913/2780182‑2780475
                                                                                                                                                    |                                                                                                                                                 
ttGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCAcc                                                                                                                                                 >  2:449080/1‑151 (MQ=255)
   tttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCAccc                                                                                                                                                <  4:394362/149‑1 (MQ=255)
   tttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCat                                                                                                                                              >  3:171851/1‑151 (MQ=255)
   tttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCa                                                                                                                                               <  1:711020/150‑1 (MQ=255)
     tttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCata                                                                                                                                             <  4:598170/150‑1 (MQ=255)
      ttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                           >  4:1575323/1‑151 (MQ=255)
      ttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                           >  1:982702/1‑151 (MQ=255)
       tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                           <  1:1125673/150‑1 (MQ=255)
       tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                           <  1:381687/150‑1 (MQ=255)
       tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                           <  3:517493/150‑1 (MQ=255)
       tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTg                                                                                                                                          >  1:397088/1‑151 (MQ=255)
        aTTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTg                                                                                                                                          >  4:1648019/1‑150 (MQ=255)
         ttGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGAc                                                                                                                                        >  4:298569/1‑151 (MQ=255)
            ccTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCtg                                                                                                                                     >  1:7848/1‑151 (MQ=255)
             ctctCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCtg                                                                                                                                     >  1:1444400/1‑150 (MQ=255)
                tcCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTgtgt                                                                                                                                  >  3:87650/1‑150 (MQ=255)
                 ccGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTTTTGTCTCATTCGCCACCCCCTCGACATTTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGttt                                                                                                                                <  1:857745/151‑1 (MQ=255)
                 ccGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGtt                                                                                                                                 >  4:1571675/1‑150 (MQ=255)
                  cGTTAGCTGAAACTTTTTGTATTCCGCCGTCTTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTTTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTgtgt                                                                                                                                  <  1:655006/148‑1 (MQ=255)
                   gTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTg                                                                                                                              <  4:487553/151‑1 (MQ=255)
                    ttAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                             >  4:72875/1‑151 (MQ=255)
                    ttAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                             >  1:540030/1‑151 (MQ=255)
                     tAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                             >  3:121619/1‑150 (MQ=255)
                     tAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                             <  2:261659/150‑1 (MQ=255)
                      aGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTc                                                                                                                            <  1:252942/150‑1 (MQ=255)
                       gCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                             <  4:793400/148‑1 (MQ=255)
                         tGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCaga                                                                                                                         >  1:706572/1‑150 (MQ=255)
                         tGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCCCGACATTTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                             <  3:344996/146‑1 (MQ=255)
                           aaaCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCagaaga                                                                                                                      <  1:430084/151‑1 (MQ=255)
                           aaaCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCaga                                                                                                                         >  1:873743/1‑148 (MQ=255)
                            aaCTGTTTGTATTCCGCGGTGGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAagag                                                                                                                     <  4:1771443/151‑1 (MQ=255)
                            aaCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCCCACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCTGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAagag                                                                                                                     <  4:109529/151‑1 (MQ=255)
                            aaCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAagag                                                                                                                     <  1:574366/151‑1 (MQ=255)
                               tGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGgtg                                                                                                                  >  2:501581/1‑151 (MQ=255)
                                gTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGgtgt                                                                                                                 >  4:989457/1‑151 (MQ=255)
                                gTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGgt                                                                                                                   <  4:1197629/149‑1 (MQ=255)
                                    gTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTg                                                                                                              <  4:1074194/150‑1 (MQ=255)
                                     tATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGAc                                                                                                            >  2:337494/1‑151 (MQ=255)
                                     tATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGAAAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCt                                                                                                               <  1:1347853/148‑1 (MQ=255)
                                        tCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTcc                                                                                                         >  4:1801870/1‑151 (MQ=255)
                                          cgccgTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCAc                                                                                                       >  1:267807/1‑151 (MQ=255)
                                              gtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                                                                   >  4:420062/1‑151 (MQ=255)
                                               tcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                                                                   >  1:145772/1‑150 (MQ=255)
                                                cgtTAATTGTTGTCTCATTCGCCACCCCCCCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTa                                                                                                  <  4:1872981/150‑1 (MQ=255)
                                                    aaTTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcg                                                                                             <  1:1609284/151‑1 (MQ=255)
                                                     aTTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgc                                                                                            >  1:1650197/1‑151 (MQ=255)
                                                       tgttgtCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgcc                                                                                           <  4:1116505/150‑1 (MQ=255)
                                                         ttgtCTCATCCCCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgccgc                                                                                         <  1:600218/150‑1 (MQ=255)
                                                               cATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGc                                                                                  >  4:1375303/1‑151 (MQ=255)
                                                                 tccGCCCCCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTg                                                                                   <  1:744512/146‑1 (MQ=255)
                                                                   cGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCAt                                                                                      <  2:4215/143‑1 (MQ=255)
                                                                   cGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcagc                                                                              >  1:1737148/1‑151 (MQ=255)
                                                                   cGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCca                                                                                <  1:160439/149‑1 (MQ=255)
                                                                     ccACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcagca                                                                             <  1:720449/150‑1 (MQ=255)
                                                                      cacaCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAgg                                                                           <  2:289901/151‑1 (MQ=255)
                                                                          cccTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGata                                                                        <  1:113929/150‑1 (MQ=255)
                                                                              cGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGtt                                                                   >  1:1500608/1‑151 (MQ=255)
                                                                                aCAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcg                                                                 <  4:546247/151‑1 (MQ=255)
                                                                                aCAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcg                                                                 <  4:909538/151‑1 (MQ=255)
                                                                                aCAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTc                                                                  <  4:1231612/150‑1 (MQ=255)
                                                                                  aaTTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgc                                                                <  4:1693359/150‑1 (MQ=255)
                                                                                   aTTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgc                                                                >  2:342126/1‑149 (MQ=255)
                                                                                        ttCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAccc                                                         >  4:989963/1‑151 (MQ=255)
                                                                                        ttCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAccc                                                         >  1:470821/1‑151 (MQ=255)
                                                                                         tctccgccgGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCa                                                            <  3:22649/144‑1 (MQ=255)
                                                                                         tCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAccc                                                         <  2:166508/150‑1 (MQ=255)
                                                                                          cACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCt                                                        <  2:217934/150‑1 (MQ=255)
                                                                                            ccgccgGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTg                                                       <  1:10821/149‑1 (MQ=255)
                                                                                              gccgGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTATCACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTTCt                                                     >  1:1396498/1‑149 (MQ=255)
                                                                                                cgGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGAt                                                 <  1:350519/151‑1 (MQ=255)
                                                                                                cgGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGAt                                                 >  3:191885/1‑151 (MQ=255)
                                                                                                 ggTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATg                                                <  3:518634/151‑1 (MQ=255)
                                                                                                  gTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGc                                               <  3:308076/151‑1 (MQ=255)
                                                                                                   tGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCt                                              >  2:81658/1‑151 (MQ=255)
                                                                                                    gAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTg                                             >  4:1399958/1‑151 (MQ=255)
                                                                                                       cgtcgtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAc                                          <  2:304099/151‑1 (MQ=255)
                                                                                                         tcgtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCTCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAc                                          <  1:1713432/149‑1 (MQ=255)
                                                                                                          cgtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAcgcg                                       >  1:965347/1‑151 (MQ=255)
                                                                                                             gCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGAc                                     <  4:754026/150‑1 (MQ=255)
                                                                                                             gCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACg                                    <  4:1444982/151‑1 (MQ=255)
                                                                                                              ccTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGc                                   >  4:810397/1‑151 (MQ=255)
                                                                                                                   cGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATcc                              >  4:1426123/1‑151 (MQ=255)
                                                                                                                    gCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcg                             >  1:570643/1‑151 (MQ=255)
                                                                                                                    gCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcg                             <  4:397228/151‑1 (MQ=255)
                                                                                                                     cAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcgc                            >  4:1236427/1‑151 (MQ=255)
                                                                                                                      aGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcgcg                           >  4:633911/1‑151 (MQ=255)
                                                                                                                      aGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcgc                            >  4:1022775/1‑150 (MQ=255)
                                                                                                                       gTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcgcgc                          <  1:1172270/151‑1 (MQ=255)
                                                                                                                            ttttgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGtt                     >  2:502817/1‑151 (MQ=255)
                                                                                                                            ttttgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGtt                     >  4:333913/1‑151 (MQ=255)
                                                                                                                              ttgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTc                    <  4:1028687/150‑1 (MQ=255)
                                                                                                                               tgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCt                  >  1:1766470/1‑151 (MQ=255)
                                                                                                                               tgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCt                  >  1:1308551/1‑151 (MQ=255)
                                                                                                                                     gACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGAcc            <  4:540247/151‑1 (MQ=255)
                                                                                                                                       cGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCcg          >  4:548555/1‑151 (MQ=255)
                                                                                                                                         aTCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCcgcg        >  1:308038/1‑151 (MQ=255)
                                                                                                                                          tctcCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGt       >  4:1123812/1‑151 (MQ=255)
                                                                                                                                          tctcCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGt       >  2:384713/1‑151 (MQ=255)
                                                                                                                                            tcCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCg     >  1:1132975/1‑151 (MQ=255)
                                                                                                                                               cGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGccc  >  3:322754/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCC  >  NC_000913/2780182‑2780475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: