BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,857,878	T→C	R262R (CGT→CGC)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,857,878	0	T	C	100.0%	493.3 / NA	134	R262R (CGT→CGC)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (66/68); total (66/68)

Only 100 of 134 total aligned reads displayed.

GAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGTGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAACGCTGGCTG  >  NC_000913/2857731‑2858025

 aaTCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGc                                                                                                                                                 >  4:168286/1‑151 (MQ=255)

  aTCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGc                                                                                                                                                 >  4:1284712/1‑150 (MQ=255)

  aTCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGc                                                                                                                                                 >  4:617533/1‑150 (MQ=255)

   tCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGc                                                                                                                                                 >  4:471731/1‑149 (MQ=255)

   tCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGc                                                                                                                                                 >  4:208444/1‑149 (MQ=255)

     tGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAgg                                                                                                                                              >  1:1736009/1‑150 (MQ=255)

        aaTTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGAc                                                                                                                                            >  4:1237100/1‑149 (MQ=255)

         aTTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGc                                                                                                                                         >  1:464494/1‑151 (MQ=255)

          tttGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGc                                                                                                                                         >  4:1303516/1‑150 (MQ=255)

            tGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGcat                                                                                                                                       <  1:1150004/150‑1 (MQ=255)

              ggACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGcatcat                                                                                                                                    <  1:649821/151‑1 (MQ=255)

              ggACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGcatcat                                                                                                                                    <  2:482397/151‑1 (MQ=255)

               gACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCAtt                                                                                                                                   <  4:1592654/151‑1 (MQ=255)

                    gcgATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGa                                                                                                                              <  1:215093/151‑1 (MQ=255)

                           ggTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGAt                                                                                                                             <  4:882378/145‑1 (MQ=255)

                           ggTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCcgc                                                                                                                        <  4:571794/150‑1 (MQ=255)

                            gTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGa                                                                                                                      <  1:415834/151‑1 (MQ=255)

                              cGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGAcg                                                                                                                    >  4:772882/1‑151 (MQ=255)

                               ggTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGAcgc                                                                                                                   >  2:159466/1‑151 (MQ=255)

                               ggTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGAcgc                                                                                                                   <  1:1502850/151‑1 (MQ=255)

                                  tttGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGcgtc                                                                                                                >  1:1176496/1‑151 (MQ=255)

                                   ttGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGcgtc                                                                                                                <  1:617275/150‑1 (MQ=255)

                                       cGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAAt                                                                                                           >  1:632845/1‑151 (MQ=255)

                                         tCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCt                                                                                                         >  1:98092/1‑151 (MQ=255)

                                           gagaACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTgg                                                                                                       <  1:1641769/151‑1 (MQ=255)

                                            agaACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGa                                                                                                      <  1:1618751/151‑1 (MQ=255)

                                             gaACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGa                                                                                                      >  3:277211/1‑150 (MQ=255)

                                               aCGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAAt                                                                                                   <  2:372573/151‑1 (MQ=255)

                                                cgcgccgcgcGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATc                                                                                                  >  3:400442/1‑151 (MQ=255)

                                                cgcgccgcgcGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATc                                                                                                  <  3:440394/151‑1 (MQ=255)

                                                cgcgccgcgcGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATc                                                                                                  >  4:145182/1‑151 (MQ=255)

                                                  cgccgcgcGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCAc                                                                                                <  4:1086045/151‑1 (MQ=255)

                                                  cgccgcgcGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCAc                                                                                                >  2:243700/1‑151 (MQ=255)

                                                    ccgcgcGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCAccc                                                                                              >  2:436976/1‑151 (MQ=255)

                                                     cgcgcgGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATTCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCAccc                                                                                              >  2:2240/1‑150 (MQ=255)

                                                     cgcgcgGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCAccc                                                                                              >  4:1878209/1‑150 (MQ=255)

                                                     cgcgcgGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCAccc                                                                                              >  1:1820995/1‑150 (MQ=255)

                                                     cgcgcgGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCa                                                                                             <  1:993894/151‑1 (MQ=255)

                                                     cgcgcgGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCa                                                                                             <  4:1031932/151‑1 (MQ=255)

                                                      gcgcgGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCAccc                                                                                              >  4:231277/1‑149 (MQ=255)

                                                      gcgcgGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAg                                                                                            <  1:442141/151‑1 (MQ=255)

                                                           gACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCt                                                                                       <  3:517656/151‑1 (MQ=255)

                                                           gACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCt                                                                                       <  1:1365148/151‑1 (MQ=255)

                                                           gACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCt                                                                                       <  4:178963/151‑1 (MQ=255)

                                                            aCTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTg                                                                                      >  1:335940/1‑151 (MQ=255)

                                                             cTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTg                                                                                      <  4:1142557/150‑1 (MQ=255)

                                                                 gtgCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCggg                                                                                 <  2:542233/151‑1 (MQ=255)

                                                                  tgctgcCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCggg                                                                                 >  3:2488/1‑150 (MQ=255)

                                                                     tgcCGGTTGTCTGGTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCgggggg                                                                              >  1:1647550/1‑150 (MQ=255)

                                                                      gcCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGggtgg                                                                              <  1:99743/149‑1 (MQ=255)

                                                                      gcCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGgtggtg                                                                            >  4:1189537/1‑151 (MQ=255)

                                                                      gcCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGgtggtg                                                                            <  2:303660/151‑1 (MQ=255)

                                                                      gcCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGgtggtg                                                                            <  4:878318/151‑1 (MQ=255)

                                                                        cGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGGGGTGCg                                                                          >  1:1263843/1‑151 (MQ=255)

                                                                          gTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCgg                                                                         <  3:170598/150‑1 (MQ=255)

                                                                             gTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACGCTGCCGCATATTCGTTCCAGCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGACGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGaaaa                                                                     <  1:1031906/151‑1 (MQ=255)

                                                                              tCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAAt                                                                    >  4:618299/1‑151 (MQ=255)

                                                                                 gTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACg                                                                 <  1:518630/151‑1 (MQ=255)

                                                                                  ttGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGc                                                                >  2:327261/1‑151 (MQ=255)

                                                                                         aTGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTc                                                         <  4:1378099/151‑1 (MQ=255)

                                                                                           gCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCtg                                                       <  2:150231/151‑1 (MQ=255)

                                                                                             gAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCTCCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCtgtg                                                     <  4:1574912/151‑1 (MQ=255)

                                                                                             gAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCtgtg                                                     >  1:1534547/1‑151 (MQ=255)

                                                                                              aaaGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGc                                                    >  4:1775531/1‑151 (MQ=255)

                                                                                                aGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTc                                                  <  4:399443/151‑1 (MQ=255)

                                                                                                 gATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCg                                                 <  1:208580/151‑1 (MQ=255)

                                                                                                  aTACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCg                                                 >  2:135105/1‑150 (MQ=255)

                                                                                                    aCCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACt                                              <  1:772549/151‑1 (MQ=255)

                                                                                                     cccAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTg                                             <  3:85500/151‑1 (MQ=255)

                                                                                                     cccAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTg                                             <  1:1483333/151‑1 (MQ=255)

                                                                                                     cccAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTg                                             >  4:806060/1‑151 (MQ=255)

                                                                                                       cAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGc                                            >  3:498539/1‑150 (MQ=255)

                                                                                                       cAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCa                                           <  3:179722/151‑1 (MQ=255)

                                                                                                        aaCGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCAc                                          >  2:349814/1‑151 (MQ=255)

                                                                                                        aaCGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCAc                                          >  4:512341/1‑151 (MQ=255)

                                                                                                             aCGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTc                                      >  1:1507411/1‑150 (MQ=255)

                                                                                                             aCGACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTc                                      >  4:1909445/1‑150 (MQ=255)

                                                                                                               gACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACg                                   >  1:225471/1‑151 (MQ=255)

                                                                                                               gACTCTGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACg                                   >  4:480599/1‑151 (MQ=255)

                                                                                                                   ctGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCg                                >  4:755178/1‑150 (MQ=255)

                                                                                                                    tGCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGa                               >  4:1497870/1‑150 (MQ=255)

                                                                                                                     gCCGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCg                                <  4:407591/148‑1 (MQ=255)

                                                                                                                      ccGCATATTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATgg                            >  4:1545872/1‑151 (MQ=255)

                                                                                                                          atatTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCa                         <  4:1507844/150‑1 (MQ=255)

                                                                                                                          atatTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAg                        <  4:239131/151‑1 (MQ=255)

                                                                                                                            atTCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGc                       <  3:231392/150‑1 (MQ=255)

                                                                                                                             ttCGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCg                     >  1:1763212/1‑151 (MQ=255)

                                                                                                                               cGTTCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTa                   <  1:104601/151‑1 (MQ=255)

                                                                                                                                 ttCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATg                 >  1:1010073/1‑151 (MQ=255)

                                                                                                                                 ttCCATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATg                 >  3:456065/1‑151 (MQ=255)

                                                                                                                                   ccATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGc                <  4:1157773/150‑1 (MQ=255)

                                                                                                                                   ccATCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCt               >  4:326377/1‑151 (MQ=255)

                                                                                                                                     aTCACCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGa             <  1:25301/151‑1 (MQ=255)

                                                                                                                                        aCCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAAc          >  4:1506988/1‑151 (MQ=255)

                                                                                                                                        aCCATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAAc          >  3:533733/1‑151 (MQ=255)

                                                                                                                                         ccATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCTCCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAAc          <  4:1379854/150‑1 (MQ=255)

                                                                                                                                         ccATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAAc          <  4:1674543/150‑1 (MQ=255)

                                                                                                                                         ccATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAACg         >  1:554696/1‑151 (MQ=255)

                                                                                                                                          cATGGAACGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAACgc        <  1:281884/151‑1 (MQ=255)

                                                                                                                                                aCGCGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAACgctggctg  >  1:1482788/1‑151 (MQ=255)

GAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGACTCTGCCGCATATTCGTTCCATCACCATGGAACGTGAGCAGGACAGCATCATTATGGATGCCGCGACGCGTCGTAATCTGGAAATCACCCAGAACCTGGCGGGTGGTGCGGAAAATACGCTGGCTTCTGTGCTCGACTGCACCGTCACGCCGATGGGCAGCCGTATGCTGAAACGCTGGCTG  >  NC_000913/2857731‑2858025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`